Method for animal cell culture

• US 5,976,833 A
• Filed: 09/19/1996
• Issued: 11/02/1999
• Est. Priority Date: 09/19/1995
• Status: Expired due to Term

• Current Assignee: Daiichi Sankyo Company Limited
• Original Assignee: Suntory Ltd (Kotobuki Realty Co., Ltd.)
• Inventors: Furukawa, Kazuaki, Ohsuye, Kazuhiro
• Primary Examiner(s): Degen, Nancy
• Assistant Examiner(s): Yucel, Irem
• Application Number:
  US08/716,533
• Time in Patent Office: 1,139 Days
• Field of Search: 435/68, 435/240, 435/69.1, 435/320.1, 435/69.7, 435/172.3, 435/71.1, 435/243, 435/352, 435/358, 435/440, 435/464, 536/23.2
• US Class Current: 435/69.1
• CPC Class Codes: C12N 2523/00 Culture process characterised by temperature
• CPC Class Codes: C12N 5/00 Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor; plant reproduction by tissue culture techniques A01H4/00
• CPC Class Codes: C12N 9/0071 acting on paired donors with incorporation of molecular oxygen (1.14)
• CPC Class Codes: C12P 21/02 having a known sequence of two or more amino acids, e.g. glutathione

Abstract

The object of the present invention is to provide a method for improving productivity in the production of useful substances by animal cells. The present invention discloses a method for animal cell culture to produce a desired substance, comprising the steps of (1) culturing animal cells at a temperature at which the animal cells can grow; and (2) culturing the animal cells at a lower temperature.

Claims

1. A method for culturing recombinant animal cells, transformed or transfected with a vector prepared by in vitro gene recombination, wherein said recombinant animal cells are not temperature sensitive mutants, to increase production of a desired product, comprising the steps of:

(1) culturing the recombinant animal cells at a temperature at which the animal cells can grow; and

then (2) culturing the recombinant animal cells at a lower temperature.

2. A method according to claim 1 wherein said recombinant animal cells are recombinant cells that exhibit greater productivity of said desired product at the lower culturing temperature than at the temperature at which the animal cells can grow.

3. A method according to claim 2 wherein said recombinant animal cells are recombinant cells derived from homeothermic animals.

4. A method according to claim 3 wherein said recombinant animal cells are recombinant cells derived from mammals.

5. A method according to claim 4 wherein said recombinant animal cells are recombinant cells derived from rodents.

6. A method according to claim 5 wherein said recombinant animal cells are recombinant cells derived from CHO (Chinese hamster ovary) cell line.

7. A method according to claim 6 wherein said animal cells are recombinant cells established by the DHFR (dihydrofolate reductase) gene amplification system.

8. A method according to claim 7 wherein said animals cells are recombinant CHO cells that produce amidating enzymes.

9. A method according to claim 1, wherein said lowered culturing temperature is less than about 37°

C.

10. A method according to claim 1, wherein said temperature at which the animal cells can grow is between about 36 and 38°

C.

11. A method according to claim 10 wherein said lowered culturing temperature is 30 to 35°

C.