Polynucleotides encoding gluS polypeptides of streptococcal pneumoniae

• US 5,976,840 A
• Filed: 10/31/1997
• Issued: 11/02/1999
• Est. Priority Date: 04/18/1997
• Status: Expired due to Fees

• Current Assignee: Smithkline Beecham Corporation (GSK plc)
• Original Assignee: Smithkline Beecham Corporation (GSK plc)
• Inventors: Wang, Min, Jaworski, Deborah D., Lawlor, Elizabeth J.
• Primary Examiner(s): Caputa, Anthony C.
• Assistant Examiner(s): NAVARRO, ALBERT MARK
• Application Number:
  US08/962,203
• Time in Patent Office: 732 Days
• Field of Search: 536/23.7, 536/23.1, 435/320.1, 435/325, 435/69.3, 435/70.1, 435/71.1, 435/252.3
• US Class Current: 435/69.3
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: C12N 9/93 Ligases (6)

Abstract

The invention provides gluS polypeptides and DNA (RNA) encoding gluS polypetides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing gluS polypeptides to screen for antibacterial compounds.

Claims

1. An isolated polynucleotide segment encoding SEQ ID NO:

2.

2. An isolated nucleic acid segment comprising a nucleotide sequence which is fully complementary to the polynucleotide of claim 1.

3. An isolated vector comprising the polynucleotide segment of claim 1.

4. An isolated vector comprising the nucleic acid segment of claim 2.

5. An isolated host cell comprising the vector of claim 3.

6. An isolated host cell comprising the vector of claim 4.

7. A process for producing a polypeptide encoded by said polynucleotide segment comprising culturing the host cell of claim 5 under conditions sufficient for the production of said polypeptide.

8. An isolated polynucleotide segment encoding a mature polypeptide expressed by a polynucleotide comprising SEQ ID NO:

1 in deposited strain NCIMB 40794.

9. An isolated nucleic acid segment comprising a nucleotide sequence which is fully complementary to the polynucleotide segment of claim 8.

10. An isolated vector comprising the polynucleotide segment of claim 8.

11. An isolated vector comprising the nucleic acid segment of claim 9.

12. An isolated host cell comprising the vector of claim 10.

13. An isolated host cell comprising the vector of claim 11.

14. A process for producing the mature polypeptide comprising culturing the host cell of claim 12 under conditions sufficient for the production of said polypeptide.

15. An isolated polynucleotide segment comprising:

a first polynucleotide sequence, wherein the first polynucleotide sequence (a) is a reference sequence that encodes the amino acid sequence set forth in SEQ ID NO;

2, or (b) is identical with the reference sequence except that, over the entire length corresponding to the reference sequence, the first polynucleotide sequence has up to three nucleotide substitutions, nucleotide deletions, or nucleotide insertions for every 100 nucleotides of the reference sequence.

16. An isolated nucleic acid segment comprising a nucleotide sequence which is fully complementary to the polynucleotide segment of claim 15.

17. An isolated vector comprising the polynucleotide segment of claim 15.

18. An isolated vector comprising the nucleic acid segment of claim 16.

19. An isolated host cell comprising the vector of claim 17.

20. An isolated host cell comprising the vector of claim 18.

21. A process for producing a polypeptide encoded by said polynucleotide segment comprising culturing the host cell of claim 19 under conditions sufficient for the production of said polypeptide.