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Method of preparing a variant of a lipolytic enzyme

  • US 5,976,855 A
  • Filed: 08/22/1996
  • Issued: 11/02/1999
  • Est. Priority Date: 02/22/1994
  • Status: Expired due to Term
First Claim
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1. A method of preparing a variant of a final lipolytic enzyme, which comprises(a) subjecting a DNA sequence encoding the fungal lipolytic enzyme to random mutagenesis,(b) expressing the mutated DNA sequence obtained in step (a) in a host cell, and(c) screening for host cells expressing a mutated lipolytic enzyme which requires lower amounts of calcium for exhibiting the same degree of activity.

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