Method of preparing a variant of a lipolytic enzyme
First Claim
1. A method of preparing a variant of a final lipolytic enzyme, which comprises(a) subjecting a DNA sequence encoding the fungal lipolytic enzyme to random mutagenesis,(b) expressing the mutated DNA sequence obtained in step (a) in a host cell, and(c) screening for host cells expressing a mutated lipolytic enzyme which requires lower amounts of calcium for exhibiting the same degree of activity.
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Abstract
The present invention relates to a method of preparing a variant of a parent lipolytic enzyme, comprising (a) subjecting a DNA sequence encoding the parent lipolytic enzyme to random mutagenesis, (b) expressing the mutated DNA sequence obtained in step (a) in a host cell, and (c) screening for host cells expressing a mutated lipolytic enzyme which has a decreased dependance to calcium and/or an improved tolerance towards a detergent or a detergent component as compared to the parent lipolytic enzyme.
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Citations
21 Claims
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1. A method of preparing a variant of a final lipolytic enzyme, which comprises
(a) subjecting a DNA sequence encoding the fungal lipolytic enzyme to random mutagenesis, (b) expressing the mutated DNA sequence obtained in step (a) in a host cell, and (c) screening for host cells expressing a mutated lipolytic enzyme which requires lower amounts of calcium for exhibiting the same degree of activity.
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20. In a method of subjecting a DNA sequence encoding a fungal lipolytic enzyme to random mutagenesis and expressing the mutated DNA sequence in a host cell, the improvement comprising screening for host cells expressing a mutated lipolytic enzyme which requires lower amounts of calcium for exhibiting the same degree of activity than the parent lipolytic enzyme.
Specification