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Immunoassays in capillary tubes

  • US 5,976,896 A
  • Filed: 07/29/1996
  • Issued: 11/02/1999
  • Est. Priority Date: 06/06/1994
  • Status: Expired due to Term
First Claim
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1. A process for screening for an analyte in a sample, which process comprisesimporting a fluid mixture into a capillary tube having a wall and coated on at least a portion of its interior surface with a substrate, wherein the fluid mixture comprises a sample suspected of containing the analyte and a reagent comprising a fluorescently-labeled conjugate that(a) binds to the analyte or to the analyte and the substrate and(b) fluoresces when irradiated with an appropriate electromagnetic signal;

  • maintaining the fluid mixture in the capillary tube for a time sufficient for binding to take place between the substrate and the fluorescently-labeled conjugate;

    removing excess fluid mixture from the capillary tube;

    externally irradiating the coated portion of the capillary tube with an electromagnetic signal sufficient to cause fluorescence of bound fluorescently labeled conjugate; and

    detecting through the capillary tube wall the resulting fluorescence occurring on the capillary tube interior surface to screen for the analyte.

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