Apparatus for the identification of free-lying cells

• US 5,978,497 A
• Filed: 09/20/1994
• Issued: 11/02/1999
• Est. Priority Date: 09/20/1994
• Status: Expired due to Term

• Current Assignee: Tripath Imaging Incorporated (Becton, Dickinson & Co)
• Original Assignee: NeoPath, Inc. (Becton, Dickinson & Co)
• Inventors: Meyer, Michael G., Oh, Seho, Lee, Shih-Jong J., Kuan, Chih-Chau L., Bannister, Wendy R., Wilhelm, Paul S.
• Primary Examiner(s): Tran, Phuoc
• Application Number:
  US08/309,250
• Time in Patent Office: 1,869 Days
• Field of Search: 382/128, 382/133, 382/134, 382/173, 382/190, 382/224, 364/413.1, 364/413.08
• US Class Current: 382/133
• CPC Class Codes: G01N 15/1433 using image recognition
• CPC Class Codes: G01N 2015/1497 Particle shape
• CPC Class Codes: G06V 20/69 Microscopic objects, e.g. biological cells or cellular parts

Abstract

A free-lying cell classifier. An automated microscope system comprising a computer and high speed processing field of view processors identifies free-lying cells. An image of a biological specimen is obtained and the image is segmented to create a set of binary masks. The binary masks are used by a feature calculator to compute the features that characterize objects of interest including free-lying cells, artifacts and other biological objects. The objects are classified to identify their type, their normality or abnormality or their identification as an artifact. The results are summarized and reported. A stain evaluation of the slide is performed as well as a typicality evaluation. The robustness of the measurement is also quantified as a classification confidence value. The free-lying cell evaluation is used by an automated cytology system to classify a biological specimen slide.

Claims

1. An apparatus for measuring reliability for object segmentation of a biological specimen comprising:

(a) means for acquiring at least one image of the biological specimen having an image output;

(b) means for image segmentation connected to the image output to detect objects of interest, wherein the means for image segmentation has a segmented object output;

(c) means for feature extraction connected to the segmented object output, wherein the means for feature extraction has a segmentation reliability feature output; and

(d) means for classification of objects connected to the segmentation reliability feature output having a classified output, where the classified output comprises a measure of reliability of the segmented object output.

2. The apparatus of claim 1 wherein the means for feature extraction comprises an object feature extractor.

3. The apparatus of claim 1 wherein the means for feature extraction comprises a contextual feature extractor.

4. The apparatus of claim 1 wherein the means for feature extraction comprises a whole image feature extractor.

5. The apparatus of claim 1 wherein the objects of interest comprise free-lying cells.

6. The apparatus of claim 1 wherein the objects of interest comprise non-nuclear overlapped cells.

7. The apparatus of claim 1 wherein the objects of interest comprise normal cells.

8. The apparatus of claim 7 wherein the normal cells comprise reference intermediate cells.

9. The apparatus of claim 1 wherein the biological specimen is a specimen prepared by the Papanicolaou method.

10. The apparatus of claim 1 wherein the biological specimen is a gynecological specimen.

11. The apparatus of claim 1 further comprising a means for accumulating the classified output.

12. The apparatus of claim 1 further comprising a means for measuring a stain of at least one type of object.

13. The apparatus of claim 12 wherein the at least one type of object comprises reference intermediate cells.

14. The apparatus of claim 1 further comprising a means for measuring a classification confidence for a set of objects classified as being object types of interest.

15. The apparatus of claim 1 wherein the objects of interest comprise abnormal cells.

16. The apparatus of claim 15 wherein the abnormal cells comprise cancerous and precancerous cells.

17. The apparatus of claim 1 wherein the objects of interest comprise artifacts.