Large pore synthetic polymer membranes

• US 5,979,670 A
• Filed: 06/11/1997
• Issued: 11/09/1999
• Est. Priority Date: 03/04/1994
• Status: Expired due to Term

• Current Assignee: USF Filtration and Separations Group, Inc. (Siemens AG)
• Original Assignee: USF Filtration and Separations Group, Inc. (Siemens AG)
• Inventors: Morris, Richard A., Ditter, Jerome, Zepf, Robert
• Primary Examiner(s): FORTUNA, ANA M
• Application Number:
  US08/872,347
• Time in Patent Office: 881 Days
• Field of Search: 210/500.34, 210/500.38, 210/500.41, 210/500.42, 210/490, 210/489, 210/500.43, 210/500.22, 422/56, 436/531
• US Class Current: 210/489
• CPC Class Codes: B01D 2323/08 Specific temperatures applied
• CPC Class Codes: B01D 2323/12 Specific ratios of components used
• CPC Class Codes: B01D 2325/022 Asymmetric membranes
• CPC Class Codes: B01D 2325/04 Characteristic thickness
• CPC Class Codes: B01D 2325/20 Specific permeability or cut-off range
• CPC Class Codes: B01D 2325/34 Molecular weight or degree of polymerisation
• CPC Class Codes: B01D 61/14 Ultrafiltration; Microfiltration
• CPC Class Codes: B01D 61/147 Microfiltration
• CPC Class Codes: B01D 67/0009 by phase separation, sol-gel transition, evaporation or solvent quenching
• CPC Class Codes: B01D 67/0011 Casting solutions therefor
• CPC Class Codes: B01D 67/0013 Casting processes
• CPC Class Codes: B01D 69/02 characterised by their properties
• CPC Class Codes: B01D 71/68 Polysulfones; Polyethersulfones
• CPC Class Codes: B32B 5/32 at least two layers being foamed and next to each other
• CPC Class Codes: C12H 1/061 Separation by centrifugation
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: G01N 33/54388 based on lateral flow
• CPC Class Codes: G01N 33/558 using diffusion or migration of antigen or antibody immunochromatographic test strips G01N33/54387

Abstract

Highly asymmetric polymeric membranes with large pores which yield bubble points in the range of 0.5 to 25 psid and superior flow characteristics. The membranes can be cast from both metastable dispersions and from homogenous casting formulations. The technique of synthesis involves exposure of the cast membrane to humid air to create large surface pores on the exposed side.

Claims

1. In a diagnostic device comprising a filtering means that delivers a filtrate that is substantially particle free containing an analyte to an analyte-detecting region of the device, the improvement comprising:

a filtering means comprising a polymer membrane, the membrane comprising a minimum porous surface, an opposite porous surface, and a porous supporting structure therebetween, wherein the minimum surface comprises a relatively open pore structure and the opposite surface comprises a more open pore structure and wherein the supporting structure comprises a high degree of asymmetry through at least 50% of the supporting structure but no more than 80% of the supporting structure, the membrane having surface pores at the minimum surface of a mean diameter of at least about 1.2 microns and having a flow rate of greater than about 4.5 cm/min/psi; and

a sample application area that directs sample first to the opposite surface.

2. The device according to claim 1, wherein the membrane has a bubble point of from about 0.5 psid to about 25 psid.

3. The device according to claim 1, wherein the membrane has a mean aqueous flow rate of from about 4.5 to 25 cm/min psid.

4. In a diagnostic device comprising a lateral wicking means that transfers a sample that is substantially particle free containing an analyte from a sample receiving region of the device to an analyte-detecting region of the device, the improvement comprising:

a lateral wicking means comprising a polymer membrane, the membrane comprising a minimum porous surface, an opposite porous surface, and a porous supporting structure therebetween, wherein the minimum surface comprises a relatively open pore structure and the opposite surface comprises a more open pore structure and wherein the supporting structure comprises a high degree of asymmetry through at least 50% of the supporting structure but no more than 80% of the supporting structure, the membrane having surface pores at the minimum surface of a mean diameter of at least about 1.2 microns and having a lateral transfer rate of greater than about 2 cm per minute; and

a sample application area that directs sample first to the opposite surface.

5. The device according to claim 4, wherein the membrane has a bubble point of from about 0.5 psid to about 25 psid.

6. The device according to claim 4, wherein the membrane has a mean aqueosus flow rate of from about 4.5 to 25 cm/min psid.

7. In a diagnostic device comprising a filtering means that delivers a filtrate that is substantially particle free containing an analyte to an analyte-detecting region of the device, the improvement comprising:

a filtering means comprising a polymer membrane, the membrane comprising a minimum porous surface, an opposite porous surface, and a porous supporting structure having a thickness therebetween, wherein the supporting structure has a generally isotropic structure from the minimum surface to a point at about one-quarter of the thickness of the supporting structure and a generally asymmetric structure from the point to the opposite surface, the membrane having surface pores at the minimum surface of a mean diameter of at least about 1.2 microns and having a flow rate of greater than about 4.5 cm/min/psi; and

a sample application area that directs sample first to the opposite surface.

8. The device according to claim 7, wherein the membrane has a bubble point of from about 0.5 psid to about 25 psid.

9. The device according to claim 7, wherein the membrane has a mean aqueous flow rate of from about 4.5 to 25 cm/min psid.

10. In a diagnostic device comprising a filtering means that delivers a filtrate that is substantially particle free containing an analyte to an analyte-detecting region of the device, the improvement comprising:

a filtering means comprising a polymer membrane, the membrane comprising a minimum porous surface, an opposite porous surface, and a supporting structure having a thickness therebetween, the supporting structure defining porous flow channels between the minimum surface and the opposite surface, wherein the flow channels have a substantially constant mean diameter from the minimum surface to a point at about one-quarter of the thickness of the supporting structure and an increasing mean diameter from the point to the opposite surface, the membrane having surface pores at the minimum surface of a mean diameter of at least about 1.2 microns and having a flow rate of greater than about 4.5 cm/min/psi; and

a sample application area that directs sample first to the opposite surface.

11. The device according to claim 10, wherein the membrane has a bubble point of from about 0.5 psid to about 25 psid.

12. The device according to claim 10, wherein the membrane has a mean aqueous flow rate of from about 4.5 to 25 cm/min psid.

13. In a diagnostic device comprising a filtering means that delivers a filtrate that is substantially particle free containing an analyte to an analyte-detecting region of the device, the improvement comprising:

a filtering means comprising a polymer membrane, the membrane comprising an integral porous skin, lying at one face of the membrane, wherein substantially all of the pores of the skin have diameters greater than about 1.2 microns, and a support region of the membrane lying below the skin and having an asymmetric structure, the membrane having a flow rate of greater than about 4.5 cm/min/psi; and

a sample application area that directs sample first to the opposite surface.

14. The device according to claim 13, wherein the membrane has a bubble point of from about 0.5 psid to about 25 psid.

15. The device according to claim 13, wherein the membrane has a mean aqueous flow rate of from about 4.5 to 25 cm/min psid.

16. In a diagnostic device comprising a filtering means that delivers a filtrate that is substantially particle free containing an analyte to an analyte-detecting region of the device, the improvement comprising:

a filtering means comprising an improved asymmetric polymer membrane having a minimum porous surface, an opposite porous surface, and a porous supporting structure therebetween and having a thickness, the membrane comprising;

a region of generally isotropic structure from the minimum surface to a point at about one-quarter of the thickness of the supporting structure, the membrane having surface pores at the minimum surface of a mean diameter of at least about 1.2 microns and having a flow rate of greater than about 4.5 cm/min/psi; and

a sample application area that directs sample first to the opposite surface.

17. The device according to claim 16, wherein the membrane has a bubble point of from, about 0.5 psid to about 25 psid.

18. The device according to claim 16, wherein the membrane has a mean aqueous flow rate of from about 4.5 to 25 cm/min psid.

19. In a diagnostic device comprising a lateral wicking means that transfers a sample that is substantially particle free containing an analyte from a sample receiving region of the device to an analyte-detecting region of the device, the improvement comprising:

a lateral wicking means comprising a polymer membrane, the membrane comprising a minimum porous surface, an opposite porous surface, and a porous supporting structure having a thickness therebetween, wherein the supporting structure has a generally isotropic structure from the minimum surface to a point at about one-quarter of the thickness of the supporting structure and a generally asymmetric structure from the point to the opposite surface, the membrane having surface pores at the minimum surface of a mean diameter of at least about 1.2 microns and having a lateral transfer rate of greater than about 2 cm per minute; and

a sample application area that directs sample first to the opposite surface.

20. The device according to claim 19, wherein the membrane has a bubble point of from about 0.5 psid to about 25 psid.

21. The device according to claim 19, wherein the membrane has a mean aqueous flow rate of from about 4.5 to 25 cm/min psid.

22. In a diagnostic device comprising a lateral wicking means that transfers a sample that is substantially particle free containing an analyte from a sample receiving region of the device to an analyte-detecting region of the device, the improvement comprising:

a lateral wicking means comprising a polymer membrane, the membrane comprising a minimum porous surface, an opposite porous surface, and a supporting structure having a thickness therebetween, the supporting structure defining porous flow channels between the minimum surface and the opposite surface, wherein the flow channels have a substantially constant mean diameter from the minimum surface to a point at about one-quarter of the thickness of the supporting structure and an increasing mean diameter from the point to the opposite surface, the membrane having surface pores at the minimum surface of a mean diameter of at least about 1.2 microns and having a lateral transfer rate of greater than about 2 cm per minute; and

a sample application area that directs sample first to the opposite surface.

23. The device according to claim 22, wherein the membrane has a bubble point of from about 0.5 psid to about 25 psid.

24. The device according to claim 22, wherein the membrane has a mean aqueous flow rate of from about 4.5 to 25 cm/min psid.

26. The device according to claim 22, wherein the membrane has a bubble point of from about 0.5 psid to about 25 psid.

27. The device according to claim 22, wherein the membrane has a mean aqueous flow rate of from about 4.5 to 25 cm/min psid.

25. In a diagnostic device comprising a lateral wicking means that transfers a sample that is substantially particle free containing an analyte from a sample receiving region of the device to an analyte-detecting region of the device, the improvement comprising:

a lateral wicking means comprising a polymer membrane, the membrane comprising an integral porous skin, lying at one face of the membrane, wherein substantially all of the pores of the skin have diameters greater than about 1.2 microns, and a support region lying below the skin and having an asymmetric structure, the membrane having a lateral transfer rate of greater than about 2 cm per minute; and

a sample application area that directs sample first to the opposite surface.

28. In a diagnostic device comprising a lateral wicking means that transfers a sample that is substantially particle free containing an analyte from a sample receiving region of the device to an analyte-detecting region of the device, the improvement comprising:

a lateral wicking means comprising a polymer membrane, the membrane comprising an improved asymmetric polymer membrane having a minimum porous surface, an opposite porous surface, and a porous supporting structure therebetween and having a thickness, the membrane comprising;

a region of generally isotropic structure from the minimum surface to a point at about one-quarter of the thickness of the supporting structure, the membrane having surface pores at the minimum surface of a mean diameter of at least about 1.2 microns and having a lateral transfer rate of greater than about 2 cm per minute; and

a sample application area that directs sample first to the opposite surface.

29. The device according to claim 28, wherein the membrane has a bubble point of from about 0.5 psid to about 25 psid.

30. The device according to claim 28, wherein the membrane has a mean aqueous flow rate of from about 4.5 to 25 cm/min psid.