Multiple emulsions comprising a hydrophobic continuous phase

• US 5,980,936 A
• Filed: 08/07/1997
• Issued: 11/09/1999
• Est. Priority Date: 08/07/1997
• Status: Expired due to Fees

• Current Assignee: Alliance Pharmaceutical Corporation
• Original Assignee: Alliance Pharmaceutical Corporation
• Inventors: Zarif, Leila, Riess, Jean G., Krafft, Marie-Pierre
• Primary Examiner(s): Page, Thurman K.
• Assistant Examiner(s): CHANNAVAJJALA, LAKSHMI SARADA
• Application Number:
  US08/908,821
• Time in Patent Office: 824 Days
• Field of Search: 424/455, 424/458, 424/450, 514/937, 514/938, 514/943
• US Class Current: 424/450
• CPC Class Codes: A61K 9/0026 Blood substitute; Oxygen transporting formulations; Plasma extender
• CPC Class Codes: A61K 9/113 Multiple emulsions, e.g. oil-in-water-in-oil A61K9/0026 takes precedence

Abstract

Multiple emulsions comprising a discontinuous emulsified phase incorporating a highly polar liquid, a second component selected from the group consisting of fluorocarbons and hydrocarbons and a continuous hydrophobic phase are disclosed. In preferred embodiments, the hydrophobic phase may comprise a fluorocarbon or hydrocarbon. Additionally, the stable multiple emulsions of the present invention may further incorporate a bioactive agent and are particularly suitable for drug delivery including pulmonary drug delivery.

Claims

1. A stable multiple emulsion for the delivery of bioactive agents comprising:

a discontinuous emulsified phase comprising a first dispersant, a polar liquid and a second component selected from the group consisting of fluorocarbons and hydrocarbon oils wherein the second component is dispersed in the polar liquid or the polar liquid is dispersed in the second component;

a continuous phase comprising a hydrophobic compound; and

an effective dispersing amount of a second dispersant wherein said discontinuous emulsified phase is immiscible in said hydrophobic compound.

2. The multiple emulsion of claim 1 wherein said hydrophobic compound is selected from the group consisting of hydrocarbons and fluorocarbons.

3. The multiple emulsion of claim 2 wherein said hydrophobic compound is a fluorocarbon selected from the group consisting of perfluoroalkanes, fluorinated cyclic compounds, halogenated fluorochemicals, fluorinated alkenes, fluorinated ethers, fluorinated polyethers, fluorinated amines, fluorinated hydrides and derivatives thereof.

4. The multiple emulsion of claim 2 wherein said hydrophobic compound is a hydrocarbon selected from the group consisting of saturated hydrocarbons, unsaturated hydrocarbons, lipids, triglycerides, natural oils, synthetic oils and derivatives thereof, said natural oils selected from the group consisting of canola oil, soybean oil, olive oil, corn oil, castor oil, sunflower oil, safflower oil and derivatives thereof.

5. The multiple emulsion of claim 1, wherein said first dispersant or said second dispersant is selected from the group consisting of fluorinated surfactants, fluorocarbon-hydrocarbon diblock compounds and combinations thereof.

6. The multiple emulsion of claim 1, wherein said first dispersant or said second dispersant is selected from the group consisting of a diblock compound selected from the group consisting of compounds having the formula C_n F_2n+1 C_m H_2m+1, compounds having the formula C_n F_2n+1 C_m H_2m-1 and combinations thereof, wherein n is an integer from 2 to 12 and m is an integer from 2 to 16 and a fluorinated surfactant selected from the group consisting of compounds having the formula R_F R₁ OP(O)[N(CH₂ CH₂)₂ O]₂, compounds having the formula [R_F R₁ O]₂ P(O)[N(CH₂ CH₂)₂ O] and combinations thereof wherein R_F is CF₃ (CF₂)_t, such that t is from 1 to 11 and R₁ is saturated or unsaturated, linear or branched hydrocarbon chain and both R_F and R₁ may contain at least one oxygen and/or sulfur atom.

7. The multiple emulsion of claim 1, wherein said first dispersant or said second dispersant is a non-fluorinated surfactant is selected from the group consisting of alcohols, salts of fatty acids, phosphatidylcholines, N-monomethyl-phosphatidylethanolamines, phosphatidic acids, phosphatidyl ethanolamines, N,N-dimethyl-phosphatidyl-ethanolamines, phosphatidyl ethylene glycols, phosphatidylmethanols, phosphatidylethanols, phosphatidylpropanols, phosphatidylbutanols, phosphatidylthioethanols, diphytanoyl phosphatides, egg yolk phospholipids, cardiolipins, isomannide monooleates, glycolipids, phosphatidylserines, phosphatidylglycerols, aminoethylphosphonolipids and combinations thereof.

8. The multiple emulsion of claim 1, wherein said multiple emulsion is selected from the group consisting of W-in-FC-in-HC emulsions, FC-in-W-in-HC emulsions, W-in-HC-in-FC emulsions and HC-in-W-in-FC emulsions.

9. The multiple emulsion of claim 1 further comprising at least one bioactive agent.

10. The multiple emulsion of claim 9, wherein said bioactive agent is selected from the group consisting of respiratory agents, bronchodilators, bronchoconstrictors, antibiotics, antivirals, mydriatics, antiglaucomas, anti-inflammatories, antihistaminics, antineoplastics, anesthetics, ophthalmic agents, enzymes, cardiovascular agents, polynucleotides, genetic material, viral vectors, immunoactive agents, imaging agents, immunosuppressive agents, peptides, proteins, physiological gases, gastrointestinal agents and combinations thereof.

19. The method of claim 1 further comprising the step of combining at least one bioactive agent with said multiple emulsion.

20. The method of claim 19 wherein said bioactive agent is selected from the group consisting of respiratory agents, bronchodilators, bronchoconstrictors, antibiotics, antivirals, mydriatics, antiglaucomas, anti-inflammatories, antihistaminics, antineoplastics, anesthetics, ophthalmic agents, enzymes, cardiovascular agents, polynucleotides, genetic material, viral vectors, immunoactive agents, imaging agents, immunosuppressive agents, peptides, proteins, physiological gases, gastrointestinal agents and combinations thereof.

11. A method for forming a stable multiple emulsion comprising the steps of:

emulsifying a first dispersant a polar liquid and a second component selected from the group consisting of fluorocarbons and hydrocarbons wherein the second component is dispersed in the polar liquid or the polar liquid is dispersed in the second component to provide an emulsified disperse phase; and

dispersing said emulsified disperse phase in a continuous phase comprising a hydrophobic compound and a second dispersant wherein said emulsified disperse phase is immiscible in said hydrophobic compound.

12. The method of claim 11 wherein said hydrophobic compound is selected from the group consisting of hydrocarbons and fluorocarbons.

13. The method of claim 12 wherein said hydrophobic compound is a fluorocarbon selected from the group consisting of perfluoroalkanes, fluorinated cyclic compounds, halogenated fluorochemicals, fluorinated alkenes, fluorinated ethers, fluorinated polyethers, fluorinated amines, fluorinated hydrides and derivatives thereof.

14. The method of claim 12 wherein said hydrophobic compound is a hydrocarbon selected from the group consisting of saturated hydrocarbons, unsaturated hydrocarbons, lipids, triglycerides, natural oils, synthetic oils and derivatives thereof, said natural oils selected from the group consisting of canola oil, soybean oil, olive oil, corn oil, castor oil, sunflower oil, safflower oil and derivatives thereof.

15. The method of claim 11, wherein said first dispersant or said second dispersant is selected from the group consisting of fluorinated surfactants, fluorocarbon-hydrocarbon diblock compounds and combinations thereof.

16. The method of claim 11, wherein said first dispersant or said second dispersant is selected from the group consisting of a diblock compound selected from the group consisting of compounds having the formula C_n F_2n+ C_m H_2m+, compounds having the formula C_n F_2n+ C_m H_2m-1 and combinations thereof, wherein n is an integer from 2 to 12 and m is an integer from 2 to 16 and a fluorinated surfactant selected from the group consisting of compounds having the formula R_F R₁ OP(O)[N(CH₂ CH₂)₂ O]₂, compounds having the formula [R_F R₁ O]₂ P(O)[N(CH₂ CH₂)₂ O] and combinations thereof wherein R_F is CF₃ (CF₂)_t, such that t is from 1 to 11 and R₁ is a saturated or unsaturated, linear or branched hydrocarbon chain and both R_F and R₁ may contain at least one oxygen and/or sulfur atom.

17. The method of claim 11, wherein said first dispersant or said second dispersant is a non-fluorinated surfactant is selected from the group consisting of alcohols, salts of fatty acids, phosphatidylcholines, N-monomethyl-phosphatidylethanolamines, phosphatidic acids, phosphatidyl ethanolamines, N,N-dimethyl-phosphatidyl-ethanolamines, phosphatidyl ethylene glycols, phosphatidylmethanols, phosphatidylethanols, phosphatidylpropanols, phosphatidylbutanols, phosphatidylthioethanols, diphytanoyl phosphatides, egg yolk phospholipids, cardiolipins, isomannide monooleates, glycolipids, phosphatidylserines, phosphatidylglycerols, aminoethylphosphonolipids and combinations thereof.

18. The method of claim 11, wherein said multiple emulsion is selected from the group consisting of W-in-FC-in-HC emulsions, FC-in-W-in-HC emulsions, W-in-HC-in-FC emulsions and HC-in-W-in-FC emulsions.

21. A method for delivering a bioactive agent to a patient in need thereof comprising:

providing a bioactive emulsion comprising a discontinuous emulsified phase having therein a first dispersant, a polar liquid and a second component selected from the group consisting of fluorocarbons and hydrocarbons wherein the second component is dispersed in the polar liquid or the polar liquid is dispersed in the second component, said emulsified phase dispersed in a continuous phase comprising a hydrophobic compound and an effective dispersing amount of a second dispersant wherein said emulsified disperse phase is immiscible in said hydrophobic compound and wherein said bioactive multiple emulsion further comprises at least one bioactive agent; and

administering said bioactive multiple emulsion to a patient.

22. The method of claim 21 wherein said hydrophobic compound is selected from the group consisting of hydrocarbons and fluorocarbons.

23. The method of claim 22 wherein said hydrophobic compound is a fluorocarbon selected from the group consisting of perfluoroalkanes, fluorinated cyclic compounds, halogenated fluorochemicals, fluorinated alkenes, fluorinated ethers, fluorinated polyethers, fluorinated amines, fluorinated hydrides and derivatives thereof.

24. The method of claim 22 wherein said hydrophobic compound is a hydrocarbon selected from the group consisting of saturated hydrocarbons, unsaturated hydrocarbons, lipids, triglycerides, natural oils, synthetic oils and derivatives thereof, said natural oils selected from the group consisting of canola oil, soybean oil, olive oil, corn oil, castor oil, sunflower oil, safflower oil and derivatives thereof.

25. The method of claim 21, wherein said first dispersant or said second dispersant is selected from the group consisting of fluorinated surfactants, fluorocarbon-hydrocarbon diblock compounds and combinations thereof.

26. The method of claim 21, wherein said first dispersant or said second dispersant is selected from the group consisting of a diblock compound selected from the group consisting of compounds having the formula C_n F_2n+1 C_m H_2m+1, compounds having the formula C_n F_2n+1 C_m H_2m-1 and combinations thereof, wherein n is an integer from 2 to 12 and m is an integer from 2 to 16 and a fluorinated surfactant selected from the group consisting of compounds having the formula R_F R₁ OP(O)[N(CH₂ CH₂)₂ O]₂, compounds having the formula [R_F R₁ O]₂ P(O)[N(CH₂ CH₂)₂ O] and combinations thereof wherein R_F is CF₃ (CF₂)_t, such that t is from 1 to 11 and R₁ is a saturated or unsaturated, linear or branched hydrocarbon chain and both R_F and R₁ may contain at least one oxygen and/or sulfur atom.

27. The method of claim 21, wherein said first dispersant or said second dispersant is a non-fluorinated surfactant is selected from the group consisting of alcohols, salts of fatty acids, phosphatidylcholines, N-monomethyl-phosphatidylethanolamines, phosphatidic acids, phosphatidyl ethanolamines, N,N-dimethyl-phosphatidyl-ethanolamines, phosphatidyl ethylene glycols, phosphatidylmethanols, phosphatidylethanols, phosphatidylpropanols, phosphatidylbutanols, phosphatidylthioethanols, diphytanoyl phosphatides, egg yolk phospholipids, cardiolipins, isomannide monooleates, glycolipids, phosphatidylserines, phosphatidylglycerols, aminoethylphosphonolipids and combinations thereof.

28. The method of claim 21, wherein said multiple emulsion is selected from the group consisting of W-in-FC-in-HC emulsions, FC-in-W-in-HC emulsions, W-in-HC-in-FC emulsions and HC-in-W-in-FC emulsions.

29. The method of claim 21, wherein said bioactive agent is selected from the group consisting of respiratory agents, bronchodilators, bronchoconstrictors, antibiotics, antivirals, mydriatics, antiglaucomas, anti-inflammatories, antihistaminics, antineoplastics, anesthetics, ophthalmic agents, enzymes, cardiovascular agents, polynucleotides, genetic material, viral vectors, immunoactive agents, imaging agents, immunosuppressive agents, peptides, proteins, physiological gases, gastrointestinal agents and combinations thereof.

30. The method of claim 21, wherein said administrating step comprises an administration technique selected from the group consisting of ocular, oral, pulmonary, rectal, synovial, subcutaneous, intramuscular, intraperitoneal, nasal, vaginal, aural and topical.