In vitro induction of dopaminergic cells
DCFirst Claim
1. A method for inducing the expression of tyrosine hydroxylase in neural cells in vitro comprising culturing mammalian CNS neural cells derived from normally non-dopaminergic, non-tumorigenic tissue in a culture medium to which has been added at least one member of the fibroblast growth factor family and at least one member of the transforming growth factor beta family wherein tyrosine hydroxylase is expressed.
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Abstract
A culture method for inducing the expression of tyrosine hydroxylase in neural cells is provided. Mammalian CNS neural cells are cultured in the presence of a fibroblast growth factor and at least one selected from a member of the transforming growth factor beta family, a feeder layer bed of cells, and cell conditioned medium. Cells cultured as provided above may be transplanted to provide dopaminergic cells to a patient. The cells may also be used in methods for drug screening.
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Citations
41 Claims
- 1. A method for inducing the expression of tyrosine hydroxylase in neural cells in vitro comprising culturing mammalian CNS neural cells derived from normally non-dopaminergic, non-tumorigenic tissue in a culture medium to which has been added at least one member of the fibroblast growth factor family and at least one member of the transforming growth factor beta family wherein tyrosine hydroxylase is expressed.
- 14. A method of providing dopaminergic cells to a patient comprising transplanting neural cells which are mammalian CNS neural cells derived from normally non-dopaminergic, non-tumorigenic tissue in a culture medium to which has been added at least one member of the fibroblast growth factor family and at least one member of the transforming growth factor beta family to induce the expression of tyrosine hydroxylase in said neural cells into said patient'"'"'s CNS.
- 19. A method of screening the effects of a drug on dopaminergic cells comprising culturing mammalian CNS neural cells derived from normally non-dopaminergic, non-tumorigenic tissue in a culture medium to which has been added at least one member of the fibroblast growth factor family and at least one member of the transforming growth factor beta family to induce the expression of tyrosine hydroxylase in said neural cells contacting said neural cells with a drug and measuring the response, selected from the group consisting of a dopaminergic response and cell survival, of said dopaminergic cells to said drug.
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32. A method for inducing the expression of tyrosine hydroxylase in neural cells in vitro comprising preparing a cell culture comprising mammalian CNS neural cells derived from normally non-dopaminergic neural tissue and a culture medium to which has been added at least one member of the fibroblast growth factor family and wherein said culture medium additionally comprises a member selected from the group consisting of a feeder layer bed of cells, cell conditioned medium, and members of the TGFβ
- family to induce the expression of tyrosine hydroxylase in said neural cells.
- View Dependent Claims (33, 34, 35, 36, 37, 38, 39, 40, 41)
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