Screening of soluble chemical compounds for their pharmacological properties utilizing transponders

• US 5,981,166 A
• Filed: 04/23/1997
• Issued: 11/09/1999
• Est. Priority Date: 04/23/1997
• Status: Expired due to Fees

• Current Assignee: PharmaSeq, Inc.
• Original Assignee: PharmaSeq, Inc.
• Inventors: Mandecki, Wlodek
• Primary Examiner(s): Leary, Louise N.
• Application Number:
  US08/842,383
• Time in Patent Office: 930 Days
• Field of Search: 435/4, 435/21, 435/968, 435/32, 435/28, 422/82.01, 342/42, 204/157.15
• US Class Current: 435/4
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00567 Transponder chips
• CPC Class Codes: B01J 2219/00702 Processes involving means for analysing and characterising the products
• CPC Class Codes: C40B 70/00 Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes
• CPC Class Codes: G01N 33/551 the carrier being inorganic
• CPC Class Codes: Y10S 435/968 High energy substrates, e.g. fluorescent, chemiluminescent, radioactive

Abstract

The invention provides a method to rapidly screen chemical compounds by delivering the compounds to the assay as a coating on transponders, rather than as powder or solution. The transponder'"'"'s function is to store data that identify the compound. The data can be decoded in any moment of the assay, and the identity of the desired compound established.

Claims

1. A method of screening for a chemical compound with a pharmaceutically useful property comprising the steps of:

(a) providing a solid phase particle having;

(i) a chemical compound associated with the solid phase particle, such association not involving a covalent bond between the chemical compound and the solid phase particle or its part, and

(ii) a tag identifying the chemical compound associated with the solid phase;

(b) adding the solid phase particle to a bioassay liquid medium;

(c) causing the dissolution of at least a part of the chemical compound associated with the solid phase particle;

(d) performing a bioassay on the bioassay liquid medium containing the chemical compound;

(e) identifying a chemical compound with a pharmaceutically useful property comprising the steps of;

(i) identifying the solid phase particle associated with the chemical compound with the pharmaceutically useful property, and

(ii) determining the identity of the chemical compound with the pharmaceutically useful property by reading the tag.

2. The method of claim 1 wherein:

(a) the tag identifying the compound is a transponder, said transponder having a serial number encoded in it; and

(b) reading the tag involves decoding the serial number of the transponder.

3. A method of coating the solid phase particle of claim 1 with a chemical compound, comprising the steps of:

(a) preparing the mixture of the chemical compound and a polymer in a solvent;

(b) coating the said solid phase particle by immersing and incubating the transponders in the said mixture;

(c) drying the solvent off.

4. The method of claim 3 wherein the solid phase particle is a transponder.

5. The method of claim 1 wherein the solid phase particle is associated with more than one chemical compound, and the decoding identifies a group of more than one chemical compounds.

7. A chemical compound identified through the use of an assay of claim 1.

8. The method of claim 1, wherein the dissolution occurs in less than 2 hours.

9. The method of claim 1, wherein the solid phase particle is coated with a coating comprising the chemical compound and a soluble polymer.

10. The method of claim 9, wherein the soluble polymer is selected from the group consisting of poly-lactic acid, alginic acid, cellulose, polyacrylamide, and an acrylic acid/acrylic anhydride copolymer.

6. The solid phase particle, comprising:

(i) a chemical compound associated with the solid phase particle, such association not involving a covalent bond between the chemical compound and the solid phase particle or its part, and

(ii) a transponder, said transponder having a serial number encoded in it, such serial number identifying the chemical compound associated with the solid phase.