×

Method of detecting and discriminating between nucleic acid sequences

  • US 5,981,176 A
  • Filed: 02/04/1994
  • Issued: 11/09/1999
  • Est. Priority Date: 06/17/1992
  • Status: Expired due to Term
First Claim
Patent Images

1. An allele specific primer having a 3'"'"' portion and a 5'"'"' portionwherein said 3'"'"' portion is complementary to a target sequence adjacent an allele specific nucleotide, andwherein said 5'"'"' portion is complementary to a preselected and randomly generated nucleic acid sequence which is different from said target sequence and which said preselected nucleic acid sequence is immobilized at a preselected location in an array of immobilized, preselected and randomly generated nucleic acid sequences on a solid support, wherein the length, base composition and percentage of each base in each of said immobilized preselected nucleic acid sequences are substantially the same to permit hybridization between said 5'"'"' portion and said preselected nucleic acid sequence to be carried out at the same temperature for all said immobilized preselected nucleic acid sequences.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×