Process of outdoor thin-layer cultivation of microalgae and blue-green algae and bioreactor for performing the process

• US 5,981,271 A
• Filed: 11/03/1997
• Issued: 11/09/1999
• Est. Priority Date: 11/06/1996
• Status: Expired due to Fees

• Current Assignee: Mikrobiologicky Ustav Akademie Ved Ceske Republiky
• Original Assignee: Mikrobiologicky Ustav Akademie Ved Ceske Republiky
• Inventors: Doucha, Jiri, Livansky, Karel
• Primary Examiner(s): Beisner, William H.
• Application Number:
  US08/963,339
• Time in Patent Office: 736 Days
• Field of Search: 435/257.1, 435/257.3, 435/292.1, 47/1.4
• US Class Current: 435/292.1
• CPC Class Codes: A01G 33/00 Cultivation of seaweed or algae
• CPC Class Codes: C12M 21/02 Photobioreactors culturing algae A01G33/00, A01H4/001, C12N1/12
• CPC Class Codes: C12M 23/04 Flat or tray type, drawers C12M23/10, C12M23/12, C12M23/16 take precedence
• CPC Class Codes: C12M 23/18 Open ponds; Greenhouse type or underground installations
• CPC Class Codes: C12M 23/50 Means for positioning or orientating the apparatus C12M41/08 takes precedence
• CPC Class Codes: C12M 29/18 External loop; Means for reintroduction of fermented biomass or liquid percolate loop type reactors for chemical or physical processes B01J19/2435
• CPC Class Codes: Y02A 40/80 in fisheries management
• CPC Class Codes: Y02P 60/20 Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2

Abstract

Process of outdoor thin-layer cultivation of algae in which suspension of algae saturated with carbon dioxide and enriched with necessary nutrients, is distributed on inclined cultivation areas where the suspension of algae is distributed on inclined cultivation areas under turbulent flow which depends on velocity of flow, on coefficient of roughness of the cultivation surfaces, on the thickness of the algal suspension layer and on inclination of the cultivation surface. Between individual cultivation areas carbon dioxide is supplied into the suspension and the suspension flowing from the lowest cultivation area is conveyed into the collecting tank from which it is pumped on the upper edge of the highest cultivation area. Bioreactor for accomplishing the mentioned process is composed of at least two individual cultivation meandering areas (1a, 1b, 1c, 1d) where the lower end of the upper area and the beginning of the next lower area, inclined in the opposite direction, are connected by channels (2) in which outlets for supply of carbon dioxide into suspension are placed.

Claims

1. A bioreactor for an outdoor thin layer cultivation of algae in a suspension of algae, consisting essentially of:

only one upper elongated and featureless cultivation area (1a) with an upper end and a lower end, the upper cultivation area having a first upper surface for receiving a flow of algae suspension, the first upper surface having an inclination and coefficient of roughness;

only one lower elongated and featureless cultivation are (1b) extending substantially parallel to the upper cultivation area, the lower cultivation area having an upper end and a lower end, the lower cultivation area having a second upper surface for receiving a flow of algae suspension, the second upper surface having an inclination and coefficient of roughness;

the inclinations of the upper and lower cultivation areas being opposite to each other and each being 1.1 to 2.5%, the coefficients of roughness each being 0.005 to 0.150 m^-1/3. s;

a channel (2) connecting the lower end of the upper cultivation area to the upper end of the lower cultivation area;

a collecting tank (4) below the lower end of the lower cultivation area;

pump means for returning algae suspension from the tank to the upper end of the upper cultivation area, the pump means supplying a layer of algae suspension along the first and second surfaces at a thickness of 5 to 18 mm and a flow velocity of 30 to 150 cm·

s^-1 for moving the suspension along the surfaces under turbulence which is cause by the inclination and the coefficient of roughness, the suspension moving from the upper to the lower cultivation areas through the channel; and

means for saturating the suspension with carbon dioxide by supplying carbon dioxide into the channel, the suspension being enriched with nutrients for the algae.

2. A bioreactor according to claim 1, including a diffusing member between the lower end of the lower cultivation area and an upper open end of the collecting tank for receiving rainwater and for receiving suspension from the lower end of the lower cultivation area.

3. A bioreactor according to claim 2, including a perforated pipe extending into the channel for supplying carbon dioxide for saturating the suspension in the channel with carbon diode.

4. A bioreactor according to claim 3, including means for supplying additional carbon dioxide into the pump means.

5. A bioreactor according to claim 4, wherein the pump means comprises a pump, a distribution pipe extending along the upper end of the upper cultivation area, the pump being connected to the distribution pipe.

6. A process for outdoor thin layer cultivation of algae in a suspension of algae, the process comprising:

providing a bioreactor which consists essentially of only one upper elongated and featureless cultivation area (1a) with an upper end and a lower end, the upper cultivation area having a first upper surface for receiving a flow of algae suspension, the first upper surface having an inclination and coefficient of roughness; and

only one lower elongated and featureless cultivation area (1b) extending substantially parallel to the upper cultivation area, the lower cultivation area having an upper end and a lower end, the lower cultivation area having a second upper surface for receiving a flow of algae suspension, the second upper surface having an inclination and coefficient of roughness, the inclinations of the upper and lower cultivation areas being opposite to each other and each being 1.1 to 2.5%, the coefficients of roughness each being 0.005 to 0.150 m^-1/3 ·

s;

a channel (2) connecting the lower end of the upper cultivation area to the upper end of the lower cultivation area;

a collecting tank (4) below the lower end of the lower cultivation area; and

pump means for returning algae suspension from the tank to the upper end of the upper cultivation area;

supplying a layer of algae suspension along the first and second surfaces at a thickness of 5 to 18 mm and a flow velocity of 30 to 150 cm·

s^-1 for moving the suspension along the surfaces under turbulence which is cause by the inclination and the coefficient of roughness, the suspension moving from the upper to the lower cultivation areas through the channel, the algae suspension being enriched with nutrients;

saturating the suspension with carbon dioxide by supplying carbon dioxide into the channel; and

using the pump means to return the suspension from the tank to the upper cultivation area.

7. A process according to claim 6, including providing a diffusing member (3) below the lower end of the lower cultivation area for receiving suspension therefrom, and above the collecting tank, the diffusing member being placed for receiving rainwater.

8. A process according to claim 7, including tilting the diffusing member for adjusting an amount of rainwater being sprayed onto the suspension before it reaches the collecting tank.

9. A process according to claim 8, including supplying additional carbon dioxide into the suspension between the collecting tank and the pumping means.