Systems and methods for detection of labeled materials
First Claim
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1. A system for detecting marked regions on a surface of a substrate, the system comprising:
- an excitation radiation source;
a focusing system for focusing radiation from said excitation radiation source onto a selected region of said surface of said substrate, said focusing system including a telecentric objective lens having a ratio of scanning field diameter to focused spot diameter of greater than about 2000, and a numerical aperture greater than about 0.2;
a reciprocating radiation direction system comprising a mirror selected from the group consisting of a galvanometric mirror, angularly oscillating mirror or a rotating polyhedral mirror for scanning said focused excitation radiation across said surface of said substrate at a rate of at least 20 image lines/second;
a detector for detecting an emission from said surface of said substrate in response to said excitation radiation, wherein said telecentric objective lens receives said emission and transmits the emission to the detector; and
a data acquisition system for recording an amount of said emission detected as a function of a position on said surface of said substrate from which said emission was emitted.
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Abstract
Labeled targets on a support synthesized with polymer sequences at known locations according to the methods disclosed in U.S. Pat. No. 5,143,854 and PCT WO 92/10092 or others, can be detected by exposing marked regions of sample to radiation from a source and detecting the emission therefrom, and repeating the steps of exposition and detection until the sample is completely examined.
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Citations
33 Claims
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1. A system for detecting marked regions on a surface of a substrate, the system comprising:
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an excitation radiation source; a focusing system for focusing radiation from said excitation radiation source onto a selected region of said surface of said substrate, said focusing system including a telecentric objective lens having a ratio of scanning field diameter to focused spot diameter of greater than about 2000, and a numerical aperture greater than about 0.2; a reciprocating radiation direction system comprising a mirror selected from the group consisting of a galvanometric mirror, angularly oscillating mirror or a rotating polyhedral mirror for scanning said focused excitation radiation across said surface of said substrate at a rate of at least 20 image lines/second; a detector for detecting an emission from said surface of said substrate in response to said excitation radiation, wherein said telecentric objective lens receives said emission and transmits the emission to the detector; and a data acquisition system for recording an amount of said emission detected as a function of a position on said surface of said substrate from which said emission was emitted. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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22. A system for detecting marked regions on a surface of a substrate, the system comprising:
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an excitation radiation source; first focusing optics for focusing said excitation radiation on said surface of said substrate in a spot having a diameter no greater than 5 μ
m, said focusing optics including a telecentric objective lens having a ratio of scanning field diameter to focused spot diameter of greater than about 2000;a reciprocating radiation direction system comprising a mirror selected from the group consisting of a galvanometric mirror, angularly oscillating mirror or a rotating polyhedral mirror for scanning said spot linearly across said surface of said substrate at a rate of at least 20 image lines/second, said spot having a travel distance of at least 10 mm; an optical train for separating emissions from said surface of said substrate from excitation radiation reflected from said surface of said substrate, said optical train including said telecentric objective lens, said telecentric objective lens receiving both said reflected excitation radiation and said emissions therethrough; and an autofocus system for automatically placing said surface of said substrate in a focal plane of said first focusing optics. - View Dependent Claims (23, 24, 25)
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26. A method of scanning a polymer array having a plurality of different polymer sequences, each of said different polymer sequences being immobilized on a surface of a substrate in a different known location, to identify which polymer sequence on said array is bound by a target molecule, the method comprising:
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focusing an excitation radiation source upon said surface of said substrate with a telecentric objective lens having a ratio of scanning field diameter to focused spot diameter of greater than about 2000; scanning said excitation radiation across said surface of said substrate at a speed of at least about 20 image lines/second said scanning comprising using a mirror selected from the group consisting of a galvanometric mirror, angularly oscillating mirror or rotating polyhedral mirror to direct said excitation radiation through said objective lens and across said substrate; collecting emissions from said surface of said substrate in response to said excitation radiation with said telecentric objective lens; collecting excitation radiation reflected from said substrate, thereby focusing said emissions; and recording said emissions as a function of a position on said surface of said substrate, said position indicating the polymer sequence on said array is bound by a target molecule. - View Dependent Claims (27, 28, 29, 30, 31, 32, 33)
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Specification