Centrifuge microscope capable of realizing polarized light observation

• US 5,982,535 A
• Filed: 08/07/1997
• Issued: 11/09/1999
• Est. Priority Date: 08/07/1997
• Status: Expired due to Term

• Current Assignee: Hamamatsu Photonics KK, Marine Biological Laboratory, Olympus Optical Corporation Limited (Olympus Corporation)
• Original Assignee: Hamamatsu Photonics KK, Marine Biological Laboratory, Olympus Optical Corporation Limited (Olympus Corporation)
• Inventors: Ichie, Koji, Takahashi, Hajime, Nagano, Chikara, Suzuki, Keisuke, Okada, Naobumi, Inoue, Shinya
• Primary Examiner(s): NGUYEN, THONG Q
• Application Number:
  US08/908,220
• Time in Patent Office: 824 Days
• Field of Search: 359/363, 359/900, 359/268-372, 359/385-390, 359/393-395
• US Class Current: 359/394
• CPC Class Codes: G02B 21/06 Means for illuminating specimens
• CPC Class Codes: G02B 21/26 Stages; Adjusting means therefor

Abstract

A centrifuge microscope includes a disk which is rotatable around a rotation axis and which is provided with a sample chamber for accommodating a sample. An observation optical system is provided which includes an objective lens which is positioned such that the sample chamber crosses an optical axis of the objective lens as the disk rotates. A pulsed laser source is provided for emitting a pulsed laser to the sample at a timing at which the sample chamber crosses the optical axis of the objective lens. And a delay time adjusting section is provided for adjusting a delay time of an emission timing of the pulsed laser in accordance with a rotational speed of the disk.

Claims

1. A centrifuge microscope comprising:

a rotation axis;

a disk which is mounted on said rotation axis and which is provided with a sample chamber for accommodating a sample;

a motor for driving the disk around the rotation axis;

an observation optical system including an objective lens which is positioned such that said sample chamber crosses an optical axis of said objective lens as said disk rotates, and a polarizer and an analyzer which are provided on the optical axis of said objective lens with said sample chamber interposed therebetween;

a pulsed laser source for emitting a pulsed laser to said sample along the optical axis of said objective lens at a timing at which said sample chamber crosses the optical axis of said objective lens;

adjusting means for adjusting an emission timing of said pulsed laser in accordance with a rotational speed of said disk, said adjusting means including a counter for determining the rotational speed of said disk, and a delay circuit for delaying the emission timing of said pulsed laser in accordance with the rotational speed determined by the counter;

a mirror provided on one of (i) the rotation axis and (ii) the disk, so as to face a given direction; and

means for emitting a laser beam to the mirror and for receiving a reflected light from said mirror, so as to output to said delay circuit a pulse corresponding to a given position of said disk.

2. The centrifuge microscope according to claim 1, further comprising at least one compensator provided between the analyzer and the polarizer.

3. The centrifuge microscope according to claim 2, further comprising an image pick-up element for picking up an observed image of said sample, and a barrier filter provided between said objective lens and said image pick-up element.

4. A centrifuge microscope comprising:

a stable axis;

a disk which is mounted on the stable axis and which is provided with a sample chamber for accommodating a samples;

a motor for driving the disk around the stable axis;

an optical system for transmission observation, said optical system including an objective lens which is positioned such that said sample chamber crosses an optical axis of said objective lens as said disk rotates, and a polarizer and an analyzer which are provided on the optical axis of said objective lens with said sample chamber interposed therebetween; and

a pulsed laser source for emitting a pulsed laser to said sample along the optical axis of said objective lens at a timing at which said sample chamber crosses the optical axis of said objective lens;

wherein said pulsed laser source sets a laser pulse width h (ns) so as to satisfy;

space="preserve" listing-type="equation">h≦

(0.61×

λ

/NA)/((2π

R×

N/60)/1000000)

where NA is a numerical aperture of the objective lens, λ

is a laser wavelength, R (mm) is a rotational radius of the sample, and N (rpm) is a maximum rotation speed of the sample.

5. A centrifuge microscope comprising:

a rotation axis;

a disk which is mounted on said rotation axis and which is provided with a sample chamber for accommodating a sample;

a motor for driving the disk around the rotation axis;

an optical system for incident light observation, said optical system including an objective lens which is positioned such that said sample chamber crosses an optical axis of said objective lens as said disk rotates, a half mirror and an analyzer which are provided on the optical axis of said objective lens, and a polarizer which is provided on an axis orthogonal to said optical axis;

a pulsed laser source for emitting a pulsed laser to said sample along the optical axis of said objective lens at a timing at which said sample chamber crosses the optical axis of said objective lens;

adjusting means for adjusting an emission timing of said pulsed laser in accordance with a rotational speed of said disk, said adjusting means including a counter for determining the rotational speed of said disk, and a delay circuit for delaying the emission timing of said pulsed laser in accordance with the rotational speed determined by the counter;

a mirror provided on one of (i) the rotation axis and (ii) the disk, so as to face a given direction; and

means for emitting a laser beam to the mirror and for receiving a reflected light from said mirror, so as to output to said delay circuit a pulse corresponding to a given position of said disk.

6. The centrifuge microscope according to claim 5, further comprising a compensator provided between said objective lens and said analyzer.

7. The centrifuge microscope according to claim 5, further comprising at least one compensator provided between said polarizer and said objective lens.

8. The centrifuge microscope according to claim 5, further comprising an image pick-up element for picking up an observed image of said sample, and a barrier filter provided between said objective lens and said image pick-up element.

9. A centrifuge microscope comprising:

a stable axis;

a disk which is mounted on the stable axis and which is provided with a sample chamber for accommodating a samples;

a motor for driving the disk around the stable axis;

an optical system for incident light observation, said optical system including an objective lens which is positioned such that said sample chamber crosses an optical axis of said objective lens as said disk rotates, a half mirror and an analyzer which are provided on the optical axis of said objective lens, and a polarizer which is provided on an axis orthogonal to said optical axis; and

a pulsed laser source for emitting a pulsed laser to said sample along the optical axis of said objective lens at a timing at which said sample chamber crosses the optical axis of said objective lens;

wherein said pulsed laser source sets a laser pulse width h (ns) so as to satisfy;

space="preserve" listing-type="equation">h≦

(0.61×

λ

/NA)/(2π

R×

N/60)/1000000)

where NA is a numerical aperture of the objective lens, λ

is a laser wavelength, R (mm) is a rotational radius of the sample, and N (rpm) is a the maximum rotation speed of the sample.