Method for quantifying substrate
First Claim
1. A method for quantifying a substrate comprising the steps of:
- bringing a solution containing an enzyme and an electron acceptor into contact with a surface of an electrode system comprising a working electrode and a counter electrode;
adding a sample solution containing a substrate to said solution containing the enzyme and the electron acceptor;
connecting said electrode system to a constant voltage source via a resistor being connected in series to said working electrode in order to apply to said electrode system a voltage which exhibits a temporary drop at said working electrode; and
measuring an electric current flowing through said electrode system for a predetermined time after the application of said voltage.
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Abstract
The present invention provides a method for quantifying a substrate having improved measurement accuracy and measurement sensitivity. According to the method for quantifying the substrate of the present invention, a constant voltage is applied to an electrode system, between a working electrode and a counter electrode, via a resistor connected in series with the electrode system after placing a sample solution containing the substrate on the electrode system, so as to oxidize an electron acceptor, which has been reduced by an enzyme reaction of the substrate, thereby determining a concentration of the substrate included in the sample solution based on an oxidizing current flowing in the course of oxidation.
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Citations
5 Claims
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1. A method for quantifying a substrate comprising the steps of:
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bringing a solution containing an enzyme and an electron acceptor into contact with a surface of an electrode system comprising a working electrode and a counter electrode; adding a sample solution containing a substrate to said solution containing the enzyme and the electron acceptor; connecting said electrode system to a constant voltage source via a resistor being connected in series to said working electrode in order to apply to said electrode system a voltage which exhibits a temporary drop at said working electrode; and measuring an electric current flowing through said electrode system for a predetermined time after the application of said voltage. - View Dependent Claims (2)
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3. A method for quantifying a substrate comprising the steps of:
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adding a sample solution containing a substrate to a reaction layer formed on a surface of an electrode system comprising a working electrode and a counter electrode, said reaction layer containing an enzyme and an electron acceptor; connecting said electrode system to a constant voltage source via a resistor being connected in series to said working electrode in order to apply to said electrode system a voltage which exhibits a temporary drop at said working electrode; and measuring an electric current flowing through said electrode system for a predetermined time after the application of said voltage. - View Dependent Claims (4, 5)
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Specification