Cryopreservation and cell culture medium comprising less than 50 mM sodium ions and greater than 100 mM choline salt
First Claim
1. A medium for storing cells, comprising a composition, which when constituted in aqueous solution includes less than about 50 mM sodium ions and choline salt being present at a concentration of at least 100 mM, and a cryoprotectant present in an amount effective to reduce intracellular ice formation during freezing of the cells.
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Accused Products
Abstract
A cell culture medium and cryopreservation medium in which sodium chloride is replaced with an organic cation, preferably choline chloride in a concentration of at least 100 mM, resulting a residual sodium ion concentration less than about 50 mM. The cryopreservation solution is suitable for cryopreservation of unfertilized oocytes, with thawed oocytes demonstrating the ability to survive, fertilize, and for the resulting embryos to proceed to full term development.
74 Citations
49 Claims
- 1. A medium for storing cells, comprising a composition, which when constituted in aqueous solution includes less than about 50 mM sodium ions and choline salt being present at a concentration of at least 100 mM, and a cryoprotectant present in an amount effective to reduce intracellular ice formation during freezing of the cells.
- 44. An aqueous medium having a mammalian cell therein, comprising a cryoprotectant in an effective amount to reduce intercellular ice formation, less than about 25 mM sodium ions, and at least 100 mM choline salt.
Specification
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- Resources
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Current AssigneeJacques Cohen
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Original AssigneeSaint Agnes Medical Center (Trinity Health)
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InventorsStachecki, James J.
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Primary Examiner(s)Saucier, Sandra E.
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Application NumberUS08/904,671Time in Patent Office837 DaysField of Search435/1.1, 435/1.3, 435/2US Class Current435/1.1CPC Class CodesA01N 1/02 Preservation of living partsA01N 1/0226 Physiologically active agen...
