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Invasive cleavage of nucleic acids

  • US 5,985,557 A
  • Filed: 11/26/1996
  • Issued: 11/16/1999
  • Est. Priority Date: 01/24/1996
  • Status: Expired due to Term
First Claim
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1. A composition comprising a cleavage structure, said cleavage structure comprising:

  • a) a target nucleic acid, said target nucleic acid comprising a first region, a second region, a third region and a fourth region, wherein said first region is located adjacent to and downstream from said second region, said second region is located adjacent to and downstream from said third region and said third region is located adjacent to and downstream from said fourth region;

    b) a first oligonucleotide having a 5'"'"' portion and a 3'"'"' portion, wherein said 5'"'"' portion of said first oligonucleotide comprises a sequence complementary to said second region of said target nucleic acid and wherein said 3'"'"' portion of said first oligonucleotide comprises nucleotides that are base-paired to said third region of said target nucleic acid; and

    c) a second oligonucleotide having a 5'"'"' portion, a central portion, and a 3'"'"' portion, wherein said 5'"'"' portion of said second oligonucleotide comprises nucleotides that are base-paired to said first region of said target nucleic acid, wherein said central portion of said second oligonucleotide comprises a sequence complementary to said second region of said target nucleic acid, and wherein said 3'"'"' portion of said second oligonucleotide consists of a single nucleotide that is not base-paired to said target nucleic acid.

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