Apparatus and method for the optical characterization of the structure and composition of a light scattering sample

• US 5,986,770 A
• Filed: 01/30/1997
• Issued: 11/16/1999
• Est. Priority Date: 07/30/1994
• Status: Expired due to Term

• Current Assignee: Roche Diagnostics GmbH (Roche Holding AG)
• Original Assignee: Roche Diagnostics GmbH (Roche Holding AG)
• Inventors: Boecker, Dirk, Hein, Heinz-Michael, Essenpreis, Matthias
• Primary Examiner(s): Pham, Hoa Q.
• Application Number:
  US08/776,379
• Time in Patent Office: 1,020 Days
• Field of Search: 356/237, 356/239, 356/338, 356/343, 356/429-431, 356/39, 600/310, 600/313, 600/473, 600/476, 600/475, 600/477, 250/341.1, 250/358.1, 250/339.2
• US Class Current: 356/446
• CPC Class Codes: A61B 5/0064 Body surface scanning
• CPC Class Codes: A61B 5/444 Evaluating skin marks, e.g. mole, nevi, tumour, scar
• CPC Class Codes: G01N 21/4795 spatially resolved investigating of object in scattering medium in vivo A61B

Abstract

Apparatus for the optical characterization of the internal structure and/or composition of a spatially extended, scattering sample comprising an arrangement of one or several light sources and one or several light detectors and a displacement sensor.

Claims

1. An apparatus for in-vivo optical analysis of internal structures of a specimen of light scattering tissue by moving said apparatus relative to a surface of the specimen, said apparatus comprising:

at least one light irradiating means for irradiating primary light into the surface of an in-vivo specimen;

at least one light detecting means for detecting secondary light from the surface of the specimen; and

displacement sensor means for sensing a relative movement between the light irradiating means and the light detecting means relative to the surface of the specimen, said displacement sensor means tracing a path on the surface of the specimen and sensing displacement when the apparatus is moved on the surface of the specimen; and

evaluation means connected to said light irradiating means, said light detecting means, and said displacement sensor means, said evaluation means processing data from said light detecting means and said displacement sensor means, and correlating the relative movement with said data from said light detecting means.

2. An apparatus as recited in claim 1, further comprising display means connected to said evaluation means, for displaying an optical image generated by the evaluation means thereupon.

3. An apparatus as recited in claim 1, wherein said displacement sensor means is coupled to said light irradiating means and said light detecting means.

4. An apparatus as recited in claim 1, wherein said evaluation means also generates an optical image from the data which is processed from the light detecting means and the light displacement sensor means.

5. An apparatus as recited in claim 1, wherein said evaluation means generates a one-or-more dimensional intensity profile from said data.

6. An apparatus as recited in claim 1, wherein said evaluation means generates a classification of a specimen type based upon the data process from the light detecting means and the displacement sensor means.

7. An apparatus as recited in claim 1, said apparatus comprising a plurality of light irradiating means.

8. An apparatus as recited in claim 1, comprising a plurality of light detecting means.

9. An apparatus as recited in claim 1, comprising a plurality of light irradiating means and a plurality of light detecting means, wherein each of said plurality of light irradiating means is paired with a corresponding one of the plurality of light detecting means.

10. An apparatus as recited in claim 9, wherein corresponding light irradiating means and light detecting means are separated by a same distance as other corresponding light irradiation means and light detection means.

11. An apparatus as recited in claim 10, wherein said same distance is between 0.5 mm and 100 mm.

12. An apparatus according to claim 9, wherein said plurality of light irradiating means and said plurality of light detecting means are disposed in a coplanar configuration.

13. An apparatus as recited in claim 1, wherein said light detecting means detects light intensity.

14. An apparatus as recited in claim 1, wherein said light detecting means detects a degree of polarization of the secondary light.

15. An apparatus as recited in claim 1, wherein said light detecting means detects a travel time of photons from the light irradiation means to the detecting means.

16. An apparatus according to claim 1, wherein said at least one light irradiating means and said at least one light detecting means are disposed in a coplanar configuration.

17. A method for in-vivo optical analysis of internal structures of a specimen of light scattering tissue, said method comprising the steps of:

irradiating primary light into a surface of a scattering in-vivo specimen with a light irradiating means;

detecting secondary light emerging from the surface of the specimen with a detecting means;

displacing said light irradiating means and said detection means relative to the surface of the specimen;

sensing a displacement of said light irradiating means and said light detecting means with respect to the surface of the specimen with a displacement sensor means, said displacement sensor means tracing a path on the surface of the specimen and sensing displacement when the displacement sensor means is moved on the surface of the specimen;

processing data from the light detecting means and the displacement sensor means; and

correlating the data from the light detecting means and the displacement sensor means.

18. A method according to claim 17, comprising a further step of generating an optical image from the correlated data.

19. A method according to claim 17, further comprising a step of generating a one-or-more dimensional intensity profile of the specimen based upon the correlated data.

20. A method according to claim 17, further comprising a step of classifying a sample type based upon the correlated data.

21. A method according to claim 17, further comprising a step of storing data from the light detecting means and the displacement sensor means prior to the correlation of the data.

22. A method according to claim 17, wherein said step of irradiating primary light includes controlling a timing of an irradiation cycle for the light irradiation means.

23. A method according to claim 17, wherein said step of irradiating primary light includes steps of irradiating light from a plurality of light irradiation means, with at least two of said plurality of light irradiation means having different irradiation cycles.

24. A method according to claim 17, wherein said step of detecting secondary light comprises a step of detecting secondary light which is reflected from the specimen.

25. A method according to claim 17, wherein said step of irradiating primary light into the sample comprises a step of irradiating light having a wavelength which is between 400 nm and 10,000 nm.

26. A method according to claim 17, wherein said step of irradiating primary light comprises irradiating light from a plurality of light irradiating means, and wherein said step of detecting light includes a step of detecting light with a plurality of light detecting means, wherein each of the plurality of light irradiating means is paired with a corresponding one of the plurality of light detecting means, and wherein a distance between corresponding light irradiating means and light detecting means is approximately a same size or smaller than a size of an inhomogeneity in the sample which is to be measured.

27. A method according to claim 17, wherein said irradiating, detecting, and evaluating steps are repeated in order to evaluate time-related changes to the sample.

28. A method according to claim 17, wherein said step of detecting secondary light comprises a detection of intensity of the secondary light.

29. A method according to claim 17, wherein said step of detecting secondary light includes a step of detecting a degree of polarization of the secondary light.

30. A method according to claim 17, wherein said step of detecting the secondary light comprises a step of detecting a travel time of photons from the light irradiation means to the detecting means.