Dual chamber disposable reaction vessel for amplification reactions
First Claim
1. A dual chamber reaction vessel comprising:
- a first chamber and a second chamber joined together via a connecting conduit, anda valve means for opening said conduit, comprising;
(a) a flexible conduit portion linking said first and second chambers having a wall portion;
(b) a substantially rigid seal piece disposed within said flexible conduit;
said seal piece providing a tight seal within said conduit portion and being held in the conduit by said wall of the conduit portion pressed against said seal piece; and
(c) an external device for constricting said conduit portion, wherein said conduit piece cooperates with said external device for constricting said conduit portion, said conduit portion positioned in relation to said external device such that relative motion between said conduit portion and said external device causes said constricting device to act on said seal piece to open said conduit portion and create a passage within said conduit portion at the point where said seal piece is located.
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Accused Products
Abstract
A reaction vessel for a nucleic acid amplification reaction has a first chamber containing an amplification reagent mix, a second chamber containing an amplification enzyme, and a fluid channel or chamber connecting the first and second chambers together. A fluid sample is introduced into the first chamber. After a denaturation and primer annealing process has occurred in the first chamber, the fluid channel is opened to allow the solution of the reagent and fluid sample to flow into the second chamber. The second chamber is maintained at an optimal temperature for the amplification reaction.
A station is described for processing test strips incorporating the reaction vessels. The station includes temperature and vacuum control subsystems to maintain proper temperatures in the reaction vessel and effectuate the transfer of the fluid from one chamber to the other in an autlomated fashion without human intervention.
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Citations
10 Claims
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1. A dual chamber reaction vessel comprising:
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a first chamber and a second chamber joined together via a connecting conduit, and a valve means for opening said conduit, comprising; (a) a flexible conduit portion linking said first and second chambers having a wall portion; (b) a substantially rigid seal piece disposed within said flexible conduit;
said seal piece providing a tight seal within said conduit portion and being held in the conduit by said wall of the conduit portion pressed against said seal piece; and(c) an external device for constricting said conduit portion, wherein said conduit piece cooperates with said external device for constricting said conduit portion, said conduit portion positioned in relation to said external device such that relative motion between said conduit portion and said external device causes said constricting device to act on said seal piece to open said conduit portion and create a passage within said conduit portion at the point where said seal piece is located. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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Specification