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Dual chamber disposable reaction vessel for amplification reactions

  • US 5,989,499 A
  • Filed: 04/02/1998
  • Issued: 11/23/1999
  • Est. Priority Date: 05/02/1997
  • Status: Expired due to Term
First Claim
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1. A dual chamber reaction vessel comprising:

  • a first chamber and a second chamber joined together via a connecting conduit, anda valve means for opening said conduit, comprising;

    (a) a flexible conduit portion linking said first and second chambers having a wall portion;

    (b) a substantially rigid seal piece disposed within said flexible conduit;

    said seal piece providing a tight seal within said conduit portion and being held in the conduit by said wall of the conduit portion pressed against said seal piece; and

    (c) an external device for constricting said conduit portion, wherein said conduit piece cooperates with said external device for constricting said conduit portion, said conduit portion positioned in relation to said external device such that relative motion between said conduit portion and said external device causes said constricting device to act on said seal piece to open said conduit portion and create a passage within said conduit portion at the point where said seal piece is located.

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