Methods for detection of molecules with affinity for MPL polypeptides

• US 5,989,833 A
• Filed: 06/02/1995
• Issued: 11/23/1999
• Est. Priority Date: 09/20/1994
• Status: Expired due to Term

• Current Assignee: Institut National De La Sante Et De La Recherche Medicale (Inserm) (Allianz Global Investors GmbH)
• Original Assignee: Institut National De La Sante Et De La Recherche Medicale (Inserm) (Allianz Global Investors GmbH)
• Inventors: Varlet, Paule, Penciolelli, Jean-Francios, Charon, Martine, Gisselbrecht, Sylvie, Tambourin, Pierre, Vigon, Isabelle, Wendling, Francoise, Souyri, Michele
• Primary Examiner(s): TENG, SALLY P
• Application Number:
  US08/460,402
• Time in Patent Office: 1,635 Days
• Field of Search: 435/7.2, 435/69.1, 435/240.1, 435/252.3, 435/254.11, 435/325, 530/350
• US Class Current: 435/7.2
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C07K 14/71 for growth factors; for growth regulators
• CPC Class Codes: C07K 14/7155 for interleukins [IL]
• CPC Class Codes: C12N 2740/10022 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: G01N 2333/15 Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
• CPC Class Codes: G01N 33/566 using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds

Abstract

Polypeptide of a growth factor receptor family, having a specific sequence and presenting all or part of the following properties: it encourages and/or is involved in the proliferation and/or differentiation of hematopoietic cell lines when obtained from the MPLV retrovirus; it is capable of acting as a hematopoietic growth factor receptor; it is recognized by antibodies directed against it. Polypeptides similar to the above. Applications in the diagnosis of the expression of said ligand polypeptide.

Claims

1. A method for detecting the binding of a molecule to a polypeptide having the amino acid sequence of SEQ ID NO:

1, said method comprising the steps of;

(a) contacting the molecule with a host cell transformed with a nucleic acid encoding the polypeptide, said host cell expressing the polypeptide at the surface of the host cell; and

(b) detecting formation of a complex between the molecule and the polypeptide.

2. A method for detecting the binding of a molecule to a polypeptide encoded by a nucleic acid having the nucleotide sequence of SEQ ID NO:

15, said method comprising the steps of;

(a) contacting the molecule with a host cell transformed with the nucleic acid, said host cell expressing a polypeptide encoded by the nucleic acid at the surface of the host cell; and

(b) detecting formation of a complex between the molecule and the polypeptide.

3. A method for detecting the binding to a polypeptide having the amino acid sequence of SEQ ID NO:

7, said method comprising;

(a) contacting the molecule with a host cell transformed with a nucleic acid encoding the polypeptide said host cell expressing the polypeptide at the surface of the host cell; and

(b) detecting formation of a complex between the molecule and the polypeptide.

4. A method for detecting the binding of a molecule to a polypeptide, said method comprising:

(a) contacting the molecule with a host cell transformed with a first nucleic acid, said first nucleic acid being hybirdizable under highly stringent conditions to the complement of a second nucleic acid having the nucleotide sequence of SEQ ID NO;

15, said first nucleic acid encoding a polypeptide which meets one of the following conditions;

(A) when the polypeptide is produced from the genome of the retrovirus MPLV, the polypeptide promotes the proliferation of hematopoietic cell lines in vitro and in vivo;

(B) when the polypeptide is produced from the genome of the retrovirus MPLV, the polypeptide promotes cellular differentation of hematopoietic cell lines;

(C) the polypeptide acts in vivo as a receptor for a hematopoietic growth factor, either at the level of ligand binding or at the level of signal transmission;

or

(D) the polypeptide is recognized by antibodies directed against a protein having the amino acid sequence of SEQ ID NO;

1, said host cell expressing the polypeptide at the surface of the host cell, and

(b) detecting formation of a complex between the molecule and the polypeptide.

5. The method of claim 4 wherein the polypeptide encoded by the first nucleic acid acts in vivo as a receptor for a hematopoietic growth factor, either at the level of ligand binding or at the level of signal transmission.

6. A method for detecting the binding of a molecule to a polypeptide having the amino acid sequence of SEQ ID NO:

12, said method comprising;

(a) contacting the molecule with a host cell transformed with a nucleic acid encoding the polypeptide, said host cell expressing the polypeptide at the surface of the host cell; and

(b) detecting formation of a complex between the molecule and the polypeptide.