DNA encoding IL-10-like homologue; related reagents

• US 5,989,867 A
• Filed: 09/22/1997
• Issued: 11/23/1999
• Est. Priority Date: 09/23/1996
• Status: Expired due to Fees

• Current Assignee: Schering Corporation (Merck & Co., Inc.)
• Original Assignee: Andrea Knappe, Bernhard Fleckenstein, Helmut Fickenscher
• Inventors: Fleckenstein, Bernhard, Knappe, Andrea, Fickenscher, Helmut
• Primary Examiner(s): Draper, Garnette D.
• Application Number:
  US08/934,959
• Time in Patent Office: 792 Days
• Field of Search: 536/23.5, 536/24.3, 438/69.5, 438/69.52, 438/252.3, 438/320.1
• US Class Current: 435/69.52
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: C07K 14/5428 IL-10

Abstract

Purified genes encoding cytokine from a mammal, reagents related thereto including purified proteins, specific antibodies, and nucleic acids encoding this molecule are provided. Methods of using said reagents and diagnostic kits are also provided.

Claims

1. An isolated or recombinant polynucleotide encoding the mature polypeptide of SEQ ID NO:

2.

3. A host cell comprising said polynucleotide of claim 1.

4. The polynucleotide of claim 1, that selectively hybridizes, under stringent hybridization wash conditions of at least 55°

C. less than 200 mM salt, to SEQ ID NO;

1.

5. The polynucleotide of claim 4, wherein said wash conditions are at least 65°

C.

6. The polynucleotide of claim 1, which:

a) is attached to a solid substrate;

b) is detectably labeled;

c) is in a sterile composition;

d) encodes an antigenic polypeptide having at least 12 amino acid residues;

or

e) is synthetically produced.

7. The polynucleotide of claim 1, that is a variant as a result of the degeneracy of the genetic code.

10. An expression or replication vector comprising, in operable linkage, said polynucleotide of claim 1.

12. The vector of claim 10, comprising the nature polypeptide coding sequence of SEQ ID NO:

1.

14. A method of producing an antigenic IL-10-like polypeptide comprising expressing said vector of claim 10, thereby producing said polypeptide;

and recovering said polypeptide.

16. The vector of claim 10, wherein said polynucleotide selectively hybridizes, under stringent hybridization wash conditions of at least 55°

C. and less than 200 mM salt, to SEQ ID NO;

1.

17. The polynucleotide of claim 16, wherein said wash conditions are at least 65°

C.

18. The vector of claim 10, that is:

a) a virus;

or

b) a plasmid.

2. An isolated or recombinant polynucleotide comprising the portion of SEQ ID NO:

1 that encodes the mature polypeptide.

8. The polynucleotide of claim 2, wherein T is U.

28. An isolated or recombinant polynucleotide that is the complement of said polynucleotide of claim 2.

9. An isolated or recombinant polynucleotide encoding a polypeptide that:

a) has a conservative amino acid substitution of a mature polypeptide of SEQ ID NO;

2;

b) is a natural allelic variant of the mature native polypeptide of SEQ ID NO;

2;

or

c) is a primate species variant of the mature native polypeptide of SEQ ID NO;

2.

11. An expression or replication vector comprising, in operable linkage, said polynucleotide of claim 9.

13. A host cell comprising said vector of claim 11.

15. The vector of claim 11, encoding a polypeptide that specifically binds polyclonal antibodies generated against the mature polypeptide of SEQ ID NO:

2.

19. The host cell of claim 13, that is:

a) a prokaryote cell;

b) E. coli;

c) an eukaryote cell;

or

d) a COS cell.

20. A method of producing antigenic IL-10-like polypeptide comprising culturing a host cell of claim 13 thereby producing said IL-10-like polypeptide;

and recovering said polypeptide.

21. The method of claim 20, wherein said vector comprises the mature polypeptide encoding portion of SEQ ID NO:

1.

22. The method of claim 21, wherein said host cell is eukaryotic.

23. The polynucleotide of claim 9, wherein T is U.

29. A method of producing an antigenic IL-10-like polypeptide, comprising culturing a host cell comprising an expression vector comprising the sequence complementary to said polynucleotide of claim 9 thereby producing said IL-10-like polypeptide;

and recovering said polypeptide.

24. A recombinant expression vector encoding amino acid residues 1 to 150 of SEQ ID NO:

2.

25. A cell comprising said vector of claim 24.

26. The vector of claim 25, comprising nucleotides 99 to 548 of SEQ ID NO:

1.

27. A method of producing a polypeptide, comprising culturing a cell of claim 26 thereby producing an IL-10-like polypeptide, and recovering said polypeptide.