Organo Luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes
First Claim
1. A luminescent semiconductor nanocrystal compound capable of linking to an affinity molecule and capable of emitting electromagnetic radiation in a narrow wavelength band when excited comprising:
- a) a semiconductor nanocrystal capable of emitting light in a narrow wavelength band when excited; and
b) one or more linking agent linked to said semiconductor nanocrystal and capable of linking to said affinity molecule.
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Accused Products
Abstract
A luminescent semiconductor nanocrystal compound is described which is capable of linking to an affinity molecule. The compound comprises (1) a semiconductor nanocrystal capable of emitting electromagnetic radiation (luminescing) in a narrow wavelength band and/or absorbing energy, and/or scattering or diffracting electromagnetic radiation--when excited by an electromagnetic radiation source (of narrow or broad bandwidth) or a particle beam; and (2) at least one linking agent, having a first portion linked to the semiconductor nanocrystal and a second portion capable of linking to an affinity molecule. The luminescent semiconductor nanocrystal compound is linked to an affinity molecule to form an organo luminescent semiconductor nanocrystal probe capable of bonding with a detectable substance in a material being analyzed, and capable of emitting electromagnetic radiation in a narrow wavelength band and/or absorbing, scattering, or diffracting energy when excited by an electromagnetic radiation source (of narrow or broad bandwidth) or a particle beam. The probe is stable to repeated exposure to light in the presence of oxygen and/or other radicals.
Further described is a process for making the luminescent semiconductor nanocrystal compound and for making the organo luminescent semiconductor nanocrystal probe comprising the luminescent semiconductor nanocrystal compound linked to an affinity molecule capable of bonding to a detectable substance. A process is also described for using the probe to determine the presence of a detectable substance in a material.
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Citations
50 Claims
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1. A luminescent semiconductor nanocrystal compound capable of linking to an affinity molecule and capable of emitting electromagnetic radiation in a narrow wavelength band when excited comprising:
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a) a semiconductor nanocrystal capable of emitting light in a narrow wavelength band when excited; and b) one or more linking agent linked to said semiconductor nanocrystal and capable of linking to said affinity molecule. - View Dependent Claims (2, 3, 4, 5, 6)
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7. An organo luminescent semiconductor nanocrystal probe capable of bonding with a detectable substance and capable of emitting electromagnetic radiation in a narrow wavelength band when excited, comprising a luminescent semiconductor nanocrystal compound linked to an affinity molecule capable of bonding to said detectable substance.
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8. An organo luminescent semiconductor nanocrystal probe capable of bonding with a detectable substance and capable of emitting electromagnetic radiation in a narrow wavelength band when excited comprising:
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a) a semiconductor nanocrystal capable of emitting electromagnetic radiation in a narrow wavelength band when excited; b) at least one linking agent linked to said semiconductor nanocrystal and having a second portion capable of linking to an affinity molecule; and c) an affinity molecule linked to said second portion of said linking agent, and capable of selectively bonding to said detectable substance; whereby treatment of a material with said organo luminescent semiconductor nanocrystal probe, and subsequent exposure of said treated material to excitation energy to determine the presence of said detectable substance within said material will excite said semiconductor nanocrystal in said organo luminescent semiconductor nanocrystal probe bonded to said detectable substance causing the emission of electromagnetic radiation of a narrow wavelength band signifying the presence, in said material, of said detectable substance bonded to said organo luminescent semiconductor nanocrystal probe. - View Dependent Claims (9, 10, 11, 12)
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13. A process for forming a luminescent semiconductor nanocrystal compound capable of lining to an affinity molecule and capable of emitting electromagnetic radiation in a narrow wavelength band when excited which comprises:
- linking together a semiconductor nanocrystal capable of emitting electromagnetic radiation in a narrow wavelength band when excited and a linking agent having a first portion linked to said semiconductor nanocrystal and a second portion capable of linking to an affinity molecule.
- View Dependent Claims (14)
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15. A process for forming an organo luminescent semiconductor nanocrystal probe capable of bonding with a detectable substance and capable of emitting electromagnetic radiation in a narrow wavelength band when excited which comprises linking a luminescent semiconductor nanocrystal compound with an affinity molecule capable of bonding with a detectable substance.
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16. A process for forming an organo luminescent semiconductor nanocrystal probe capable of bonding with a detectable substance and capable of emitting electromagnetic radiation in a narrow wavelength band when excited which comprises the steps of:
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a) linking a semiconductor nanocrystal capable of emitting electromagnetic radiation in a narrow wavelength band when excited with a linking agent having a first portion linked to said semiconductor nanocrystal and a second portion capable of linking to an affinity molecule; and b) linking said linking agent and an affinity molecule capable of bonding with said detectable substance. - View Dependent Claims (17, 18, 19)
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20. A process for treating a material to determine the presence of one or more detectable substances in said material which comprises:
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a) contacting said material with a first organo luminescent semiconductor nanocrystal probe capable of bonding with a first detectable substance, if present, in said material, and capable of emitting electromagnetic radiation in a first narrow wavelength band when excited, said first organo luminescent semiconductor nanocrystal probe comprising; i) a first semiconductor nanocrystal capable of being excited over a broad bandwidth and capable of emitting electromagnetic radiation in said first narrow wavelength band when excited; ii) an affinity molecule capable of selectively bonding to said detectable substance; and iii) a linking agent linked to said first semiconductor nanocrystal and also linked to said affinity molecule; b) removing, from said material, portions of said first organo luminescent semiconductor nanocrystal probe not bonded to said first detectable substance; and c) exposing said material to energy capable of exciting said first semiconductor nanocrystal to emit electromagnetic radiation in said first narrow wavelength band, indicative of the presence of said first detectable substance in said material; and d) detecting said electromagnetic radiation in said first narrow wavelength band emitted by said first semiconductor nanocrystal in said first organo luminescent semiconductor nanocrystal probe. - View Dependent Claims (21, 22, 23, 24, 25, 26, 27)
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28. A process for treating a material to determine the presence of a detectable substance in said material which comprises:
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a) contacting said material with an organo luminescent semiconductor nanocrystal probe capable of bonding with a first detectable substance, if present, in said material, and capable of absorbing energy when exposed to said energy, said organo luminescent semiconductor nanocrystal probe comprising; i) a semiconductor nanocrystal capable of absorbing energy over a broad bandwidth when exposed to said energy; ii) an affinity molecule capable of selectively bonding to said detectable substance; and iii) a linking agent linked to said first semiconductor nanocrystal and also linked to said affinity molecule; b) removing, from said material, portions of said organo luminescent semiconductor nanocrystal probe not bonded to said first detectable substance; and c) exposing said material to energy capable of being absorbed by said first semiconductor nanocrystal, indicative of the presence of said first detectable substance in said material; and d) detecting the change in absorbed energy, indicative of the presence of said organo luminescent semiconductor nanocrystal probe in said material bonded to said detectable substance. - View Dependent Claims (29, 30, 31)
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32. A process for treating a material to determine the presence of a detectable substance in said material which comprises:
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a) contacting said material with an organo luminescent semiconductor nanocrystal probe capable of bonding with a first detectable substance, if present, in said material, and capable of scattering or diffracting energy when excited, said organo luminescent semiconductor nanocrystal probe comprising; i) a semiconductor nanocrystal capable of scattering or diffracting energy over a broad bandwidth with a characteristic cross-section; ii) an affinity molecule capable of selectively bonding to said detectable substance; and iii) a linking agent linked to said first semiconductor nanocrystal and also linked to said affinity molecule; b) removing, from said material, portions of said organo luminescent semiconductor nanocrystal probe not bonded to said first detectable substance; and c) exposing said material to energy capable of exciting said first semiconductor nanocrystal to scatter or diffract energy, indicative of the presence of said first detectable substance in said material; and d) detecting the change in scattered or diffracted energy, indicative of the presence of said organo luminescent semiconductor nanocrystal probe in said material bonded to said detectable substance. - View Dependent Claims (33, 34, 35, 36, 37, 38)
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39. A luminescent semiconductor nanocrystal compound capable of linking to an affinity molecule and capable of absorbing energy in a narrow wavelength band comprising:
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a) a semiconductor nanocrystal capable of absorbing energy in a narrow wavelength band; and b) at least one linking agent linked to said semiconductor nanocrystal and capable of linking to said affinity molecule.
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40. A luminescent semiconductor nanocrystal compound capable of linking to an affinity molecule and capable of scattering or diffracting energy in a narrow wavelength band when excited comprising:
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a) a semiconductor nanocrystal capable of scattering or diffracting energy in a narrow wavelength band when excited; and b) at least one linking agent linked to said semiconductor nanocrystal and capable of linking to said affinity molecule.
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41. An organo luminescent semiconductor nanocrystal probe capable of bonding with a detectable substance and capable of absorbing energy in a narrow wavelength band, comprising a luminescent semiconductor nanocrystal compound linked to an affinity molecule capable of bonding to said detectable substance.
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42. An organo luminescent semiconductor nanocrystal probe capable of bonding with a detectable substance and capable of absorbing energy in a narrow wavelength band comprising:
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a) a semiconductor nanocrystal capable of absorbing energy in a narrow wavelength band; b) at least one linking agent linked to said semiconductor nanocrystal and having a second portion capable of lining to an affinity molecule; and c) an affinity molecule linked to said second portion of said linking agent, and capable of selectively bonding to said detectable substance; whereby treatment of a material with said organo luminescent semiconductor nanocrystal probe, and subsequent exposure of said treated material to energy to determine the presence of said detectable substance within said material will cause said semiconductor nanocrystal in said organo luminescent semiconductor nanocrystal probe, bonded to said detectable substance, to absorb energy of a narrow wavelength band signifying the presence, in said material, of said detectable substance bonded to said organo luminescent semiconductor nanocrystal probe.
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43. An organo luminescent semiconductor nanocrystal probe capable of bonding with a detectable substance and capable of scattering or diffracting energy in a narrow wavelength band when excited, comprising a luminescent semiconductor nanocrystal compound linked to an affinity molecule capable of bonding to said detectable substance.
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44. An organo luminescent semiconductor nanocrystal probe capable of bonding with a detectable substance and capable of scattering or diffracting energy in a narrow wavelength band when excited comprising:
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a) a semiconductor nanocrystal capable of scattering or diffracting energy in a narrow wavelength band when excited; b) at least one linking agent linked to said semiconductor nanocrystal and having a second portion capable of linking to an affinity molecule; and c) an affinity molecule linked to said second portion of said linking agent, and capable of selectively bonding to said detectable substance; whereby treatment of a material with said organo luminescent semiconductor nanocrystal probe, and subsequent exposure of said treated material to excitation energy to determine the presence of said detectable substance within said material will excite said semiconductor nanocrystal in said organo luminescent semiconductor nanocrystal probe bonded to said detectable substance causing the scattering or diffracting of energy of a narrow wavelength band signifying the presence, in said material, of said detectable substance bonded to said organo luminescent semiconductor nanocrystal probe.
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45. A process for forming a luminescent semiconductor nanocrystal compound capable of linking to an affinity molecule and capable of absorbing energy in a narrow wavelength band when excited which comprises:
- linking together a semiconductor nanocrystal capable of absorbing energy in a narrow wavelength band and a linking agent having a first portion linked to said semiconductor nanocrystal and a second portion capable of linking to an affinity molecule.
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46. A process for forming a luminescent semiconductor nanocrystal compound capable of linking to an affinity molecule and capable of scattering or diffracting energy in a narrow wavelength band when excited which comprises:
- linking together a semiconductor nanocrystal capable of scattering or diffracting energy in a narrow wavelength band when excited and a linking agent having a first portion linked to said semiconductor nanocrystal and a second portion capable of linking to an affinity molecule.
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47. A process for forming an organo luminescent semiconductor nanocrystal probe capable of bonding with a detectable substance and capable of absorbing energy in a narrow wavelength band which comprises linking a luminescent semiconductor nanocrystal compound with an affinity molecule capable of bonding with a detectable substance.
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48. A process for forming an organo luminescent semiconductor nanocrystal probe capable of bonding with a detectable substance and capable of absorbing energy in a narrow wavelength band which comprises the steps of:
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a) linking a semiconductor nanocrystal capable of absorbing energy in a narrow wavelength band with a liing agent having a first portion linked to said semiconductor nanocrystal and a second portion capable of linking to an affinity molecule; and b) linking said linking agent and an affinity molecule capable of bonding with said detectable substance.
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49. A process for forming an organo luminescent semiconductor nanocrystal probe capable of bonding with a detectable substance and capable of scatterin or diffracting energy in a narrow wavelength band when excited which comprises linking a luminescent semiconductor nanocrystal compound with an affinity molecule capable of bonding with a detectable substance.
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50. A process for forming an organo luminescent semiconductor nanocrystal probe capable of bonding with a detectable substance and capable of scattering or diffracting energy in a narrow wavelength band when excited which comprises the steps of:
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a) linking a semiconductor nanocrystal capable of scattering or diffracting energy in a narrow wavelength band when excited with a linking agent having a first portion linked to said semiconductor nanocrystal and a second portion capable of linking to an affinity molecule; and b) linking said linking agent and an affinity molecule capable of bonding with said detectable substance.
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Specification