Capacitive denaturation of nucleic acid
First Claim
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1. A method for denaturing a nucleic acid in a composition contained in a vessel having an interior surface composed of a dielectric material, comprising:
- subjecting the composition to capacitive charging by applying voltage across a first electrode separated from the composition by the interior surface, thereby defining a capacitor area, and a second electrode inserted into the composition; and
thereby denaturing the nucleic acid.
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Abstract
The present invention relates to the denaturation of nucleic acids using capacitive charging. In a preferred embodiment, the invention effects such denaturation in the context of a microfluidic device for the analysis, amplification, or other automated manipulation of nucleic acids.
228 Citations
18 Claims
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1. A method for denaturing a nucleic acid in a composition contained in a vessel having an interior surface composed of a dielectric material, comprising:
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subjecting the composition to capacitive charging by applying voltage across a first electrode separated from the composition by the interior surface, thereby defining a capacitor area, and a second electrode inserted into the composition; and thereby denaturing the nucleic acid. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 12)
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10. The method of claim 10, wherein the nucleic acid is DNA or RNA.
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11. The method of claim 11, wherein the enzyme is a ligase or a polymerase.
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13. A method for denaturing a nucleic acid in a composition in a first chamber (i) having an interior surface composed of a dielectric material and (ii) that is in communication with one or more second chambers, the method comprising;
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subjecting a composition comprising the nucleic acid to capacitive charging by applying voltage across a first electrode separated from the composition by the interior surface, thereby defining a capacitor area, and a second electrode inserted into the composition; and thereby denaturing the nucleic acid. - View Dependent Claims (14, 15, 16, 17, 18)
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Specification