Method of purifying and identifying a large multiplicity of chemical reaction products simultaneously

• US 5,993,662 A
• Filed: 08/28/1998
• Issued: 11/30/1999
• Est. Priority Date: 08/28/1998
• Status: Expired due to Fees

• Current Assignee: Thetagen, Inc. (Curia Global Incorporated)
• Original Assignee: Thetagen, Inc. (Curia Global Incorporated)
• Inventors: Schultz, Lauri Marie, Cameron, Lynn Michele, Garr, Cheryl Denise, Schedin, David R.
• Primary Examiner(s): Therkorn, Ernest G.
• Application Number:
  US09/143,163
• Time in Patent Office: 459 Days
• Field of Search: 210/656, 210/659, 210/198.2, 364/528.03, 364/528.08, 422/70, 422/187, 436/43, 436/161, 702/27
• US Class Current: 506/6
• CPC Class Codes: G01N 2030/027 Liquid chromatography
• CPC Class Codes: G01N 2030/8804 automated systems
• CPC Class Codes: G01N 30/8651 Recording, data aquisition, archiving and storage
• CPC Class Codes: G01N 30/88 Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
• CPC Class Codes: Y10T 436/11 Automated chemical analysis

Abstract

The invention provides a system utilizing computer databases, high pressure liquid chromatography and mass spectrometry to isolate, purify and identify compounds present in a chemical library of reaction products, especially products of liquid phase (solution) chemical reaction, to produce a purified chemical library with each purified compound tracked to the reaction product from which it originated.

Claims

1. A system of simultaneously purifying a multiplicity of solutions of chemical reaction products using liquid chromatography and identifying sought-after products using mass spectrometry, while tracking chemicals present in individual reaction products using computer memory, the system comprising:

(a) selecting an array of a multiplicity of reaction tubes, each tube containing a solution, of a chemical reaction product, each tube coded with an identifying code, the code stored in a memory of a digital signal processor to identify the chemical reaction product;

(b) subjecting at least some of the multiplicity of reaction products separately and simultaneously to liquid chromatography to produce a chromatograph for each, the chromatograph comprising a series of peaks representing individual chemical compounds contained in each reaction product;

(c) automatically collecting individual chemical compounds corresponding to peaks of the chromatographs of the plurality of the reaction products into an array of separate purified chemical compound tubes and coding with a second code, traceable to the identifying code of the reaction product from which the purified compound is obtained, the second code stored in a computer database;

(d) simultaneously sampling a multiplicity of the purified compound tubes while maintaining tracking correspondence between each sample and each purified compound tube and subjecting each of the samples separately to mass spectrometric analysis; and

(e) comparing output from the mass spectrometric analysis for each sample with data identifying known compounds expected to be present in the reaction products, the data stored in a database of a digital signal processor, to identify samples as containing particular chemical compounds.

2. The system of claim 1, further comprising removing solvent from each of the purified compound tubes to provide a chemical library of dried purified compounds, each identified by its location in an array and tracking date in a database.

3. The system of claim 2, further comprising determining the mass of the dried purified compound produced in each of the purified compound tubes.

4. The system of claim 3, further comprising sorting the purified compound tubes to separate tubes containing sought-after products from other tubes, the sorting based on comparing mass spectrometric analysis output data with data stored in a digital signal processor memory identifying each sought-after compound by molecular weight and molecular formula.

5. The system of claim 4, further comprising evaporating solvent from each of the purified compound tubes, determining a mass of each purified compound, and arranging the purified compound tubes in order of descending mass of purified compound contained therein.

6. The system of claim 5, further comprising determining, by reference to a computer database storing mass and molecular weight of each purified compound, an amount of solvent to be added to each purified compound tube to produce solutions of equal concentration in each tube of the array of purified compound tubes.

7. The system of claim 6, further comprising sampling each of the purified compound containers;

placing each sample into a separate well of a microtiter plate corresponding to a location of a purified sample in the array, while tracking and maintaining a record of each well location on the plate and chemical compound inserted into each well; and

drying samples in the wells to produce a dried chemical library of purified compounds on the microtiter plate.

8. A system of purifying a multiplicity of solutions of chemical reaction products using liquid chromatography and identifying sought-after products using mass spectrometry, while tracking chemicals present in individual reaction products using databases, to prepare a purified chemical library, the system comprising:

(a) selecting an array of a multiplicity of chemical reaction products, each reaction product of the array coded with an identifying code, the code stored in a memory of a computer to identify the chemical reaction product by location in the array;

(b) subjecting a multiplicity of the multiplicity of reaction products separately to liquid chromatography to produce a chromatograph for each of the reaction products so subjected, each chromatograph comprising peaks representing individual chemical compounds contained in a particular reaction product;

(c) automatically collecting chemical solution corresponding to peaks of the chromatographs into an array of purified chemical compound tubes, the tubes coded with a second code traceable to the identifying code of the chemical reaction product from which the purified chemical compound originates and the peak of the chromatograph to which said purified compound corresponds, the second code stored in a computer database;

(d) sampling a multiplicity of the purified compound tubes, while maintaining tracking correspondence between each sample and each purified compound tube, and subjecting each of the samples separately to mass spectrometry analysis;

(e) comparing output from the mass spectrometry analysis for each sample with data identifying known compounds expected to be present in the reaction product from which a particular purified compound originates, to identify samples as containing particular chemical compounds;

(f) sorting identified sought-after compounds of the purified compounds into an array;

(g) evaporating solvent from the sorted array of compounds, to produce and array of dried compounds;

(h) determining the mass of each sorted dried compound, and rearranging the compounds in accordance with a pre-selected criterion, while maintaining tracking correspondence between each of the dried purified compounds and the reaction product from which it originates;

(i) adding a predetermined amount of solvent to the dried purified compounds to produce an array of equal concentration solutions of purified compounds;

(j) sampling the solutions of purified compounds, and dispensing samples into microtiter plates, while maintaining tracking correspondence between purified sample containers and microtiter wells, to produce a chemical library of dried purified compounds, each compound identified by its location in the an array of wells of the microtiter plate and tracking data in a database.