Growth/differentiation factor of the TGF-.beta. family

• US 5,994,094 A
• Filed: 08/10/1994
• Issued: 11/30/1999
• Est. Priority Date: 08/10/1993
• Status: Expired due to Term

• Current Assignee: Biopharm Gesellschaft Zur Biotecnologischen
• Original Assignee: Biopharm Gesellschaft Zur Biotecnologischen
• Inventors: Hotten, Gertrud, Neidhardt, Helge, Paulista, Michael
• Primary Examiner(s): Kemmerer, Elizabeth
• Assistant Examiner(s): Romeo, David S.
• Application Number:
  US08/288,508
• Time in Patent Office: 1,938 Days
• Field of Search: 435/69.4, 435/320.1, 435/252.3, 435/254.11, 536/23.5, 436/69.1
• US Class Current: 435/69.1
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61P 1/02 Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
• CPC Class Codes: A61P 17/00 Drugs for dermatological disorders
• CPC Class Codes: A61P 17/02 for treating wounds, ulcers, burns, scars, keloids, or the like
• CPC Class Codes: A61P 19/00 Drugs for skeletal disorders
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61P 5/00 Drugs for disorders of the endocrine system
• CPC Class Codes: A61P 9/00 Drugs for disorders of the cardiovascular system
• CPC Class Codes: C07K 14/51 Bone morphogenetic factor; Osteogenins; Osteogenic factor; Bone-inducing factor

Abstract

The invention concerns a protein of the TGF-β family, the DNA coding therefor and a pharmaceutical composition containing the protein.

Claims

1. An isolated DNA molecule which codes for a protein of the TGF-β

family, wherein said DNA molecule comprises a sequence selected from the group consisting of;

a) the sequence shown in SEQ ID NO;

1,

b) a part of SEQ ID NO;

1 which encodes the mature protein,

c) a nucleotide sequence which encodes the amino acid sequence according to SEQ ID NO;

2,

d) a nucleotide sequence which encodes a portion of the amino acid sequence according to SEQ ID NO;

2, wherein said portion is the mature protein, and

e) a part of SEQ ID NO;

1 which encodes the amino acid sequence according to SEQ ID NO;

13.

2. The DNA molecule according to claim 1, wherein said DNA molecule is the part of SEQ ID NO:

1 which encodes the mature protein and further comprises nucleotide sequences coding for functional signal and/or propeptide sequences from a protein of the TGF-β

family.

3. The DNA molecule according to claim 2, wherein said functional signal and/or propeptide sequences of said DNA molecule are the functional signal and/or propeptide sequences of SEQ ID NO:

2.

4. The DNA molecule according to claim 3, further comprising a non-coding intron between nucleotides 1270 and 1271 of SEQ ID NO:

1.

5. The DNA molecule according to claim 1, wherein said DNA molecule comprises nucleotides 640-2142 of SEQ ID NO:

1.

6. A vector comprising at least one copy of a DNA molecule according to claim 1.

7. A host cell transformed by a vector according to claim 6.

8. The host cell according to claim 7, wherein said host cell is selected from the group consisting of a bacterium, a fungal cell, a plant cell, and an animal cell.

9. A process for the production of a protein of the TGF-β

family, comprising the steps of;

culturing a host cell according to claim 7 in a culture media, and

isolating TGF-β

protein from said host cell or from said culture media.

10. An isolated DNA molecule comprising nucleotide sequences coding for the functional signal and/or propeptide sequence of SEQ ID NO:

2.