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Portable cassette for use in maintaining and growing biological cells

  • US 5,994,129 A
  • Filed: 07/08/1997
  • Issued: 11/30/1999
  • Est. Priority Date: 06/07/1995
  • Status: Expired due to Term
First Claim
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1. A portable cell growth apparatus for maintaining and growing biological cells, comprising:

  • a first casing that defines a cell growth chamber configured to carry a quantity of biological cells and growth media;

    a media container connected to the cell growth chamber and configured to carry growth media;

    a waste container connected to the cell growth chamber and configured to carry media discharged from the growth chamber;

    wherein the cell growth chamber, the media container, and the waste container are connected together to form a closed system configured to be sterilizable as a unit; and

    a flow control device that is mechanically engageable with an external mechanical control system, for transporting growth media through the cell growth chamber, from the media container to the waste container, without exposing the closed, sterile system to the external environment;

    and wherein the cell growth chamber is configured to be inoculated with biological cells, and thereafter to maintain and grow the inoculated biological cells while the flow control device, in response to the external mechanical control system transports growth media through the cell growth chamber, from the media container to the waste container, without exposing the closed, sterile system to the external environment.

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