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Use of porphyrins in instrumental detection methods

  • US 5,998,128 A
  • Filed: 06/13/1997
  • Issued: 12/07/1999
  • Est. Priority Date: 06/14/1996
  • Status: Expired due to Fees
First Claim
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1. A process for quantifying entities having a hydrophobic part or a part capable of hydrophobic interaction, comprising the steps of(a) mixing an effective detection amount of a (proto-) porphyrin represented by formula (I) ##STR6## wherein R1 independently represents a radical selected from the group consisting of --CH(OH)--CH3, --CH═

  • CH2, --CH2 --CH3, --H, --COCH3, --CHO, --CH(OH)--CH2 OH, and --CH═

    CHO2 H;

    R2 independently is selected from C1-3 alkyl;

    R3 independently represents an aryl or aralkyl group; and

    M is a metal selected from Fe, Co, Ga, Sn, Zn, - Cr, Mg, Ni, Ge, and Cu,with a sample suspected to contain entities to be detected;

    (b) collecting the (proto)porphyrin containing complexes formed; and

    (c) detecting and quantifying the collected complexes by chemiluminescence, radiometric detection, or fluorimetric detection.

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