Use of porphyrins in instrumental detection methods
First Claim
1. A process for quantifying entities having a hydrophobic part or a part capable of hydrophobic interaction, comprising the steps of(a) mixing an effective detection amount of a (proto-) porphyrin represented by formula (I) ##STR6## wherein R1 independently represents a radical selected from the group consisting of --CH(OH)--CH3, --CH═
- CH2, --CH2 --CH3, --H, --COCH3, --CHO, --CH(OH)--CH2 OH, and --CH═
CHO2 H;
R2 independently is selected from C1-3 alkyl;
R3 independently represents an aryl or aralkyl group; and
M is a metal selected from Fe, Co, Ga, Sn, Zn, - Cr, Mg, Ni, Ge, and Cu,with a sample suspected to contain entities to be detected;
(b) collecting the (proto)porphyrin containing complexes formed; and
(c) detecting and quantifying the collected complexes by chemiluminescence, radiometric detection, or fluorimetric detection.
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Abstract
The present invention relates to a process for quantifying entities, comprising the steps of
(a) mixing an effective detection amount of a (proto-) porphyrin represented by formula (I) ##STR1## wherein R1 independently represents a radical selected from the group consisting of --CH(OH)--CH3, --CH═CH2, --CH2 --CH3, --H, --COCH3, --CHO, --CH(OH)--CH2 OH, and --CH═CHO2 H;
R2 independently is selected from C1-3 alkyl, and preferably is methyl,
R3 independently represents an aryl or aralkyl group, and preferably is phenyl; and
M is a metal selected from Fe, Co, Ga, Sn, Zn, Cr, Mg, Ni, Ge, and Cu,
with a sample suspected to contain entities to be detected;
(b) collecting the (proto)porphyrin containing complexes formed; and
(c) detecting and quantifying the collected complexes.
Further, the invention relates to a chemiluminescent composition comprising a compound defined in formula I.
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Citations
11 Claims
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1. A process for quantifying entities having a hydrophobic part or a part capable of hydrophobic interaction, comprising the steps of
(a) mixing an effective detection amount of a (proto-) porphyrin represented by formula (I) ##STR6## wherein R1 independently represents a radical selected from the group consisting of --CH(OH)--CH3, --CH═ - CH2, --CH2 --CH3, --H, --COCH3, --CHO, --CH(OH)--CH2 OH, and --CH═
CHO2 H;R2 independently is selected from C1-3 alkyl; R3 independently represents an aryl or aralkyl group; and M is a metal selected from Fe, Co, Ga, Sn, Zn, - Cr, Mg, Ni, Ge, and Cu, with a sample suspected to contain entities to be detected; (b) collecting the (proto)porphyrin containing complexes formed; and (c) detecting and quantifying the collected complexes by chemiluminescence, radiometric detection, or fluorimetric detection. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
- CH2, --CH2 --CH3, --H, --COCH3, --CHO, --CH(OH)--CH2 OH, and --CH═
Specification