Enzymatic nucleic acids containing 5'-and/or 3'-cap structures
First Claim
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1. An enzymatic nucleic acid molecule comprising a 5'"'"'-cap structure, a 3'"'"'-cap structure, or both a 5'"'"'- and a 3'"'"'-cap structure, wherein said 5'"'"'-cap structure is selected from the group consisting of 4'"'"',5'"'"'-methylene nucleotide;
- 1-(β
-D-erythrofuranosyl)nucleotide;
4'"'"'-thio nucleotide, carbocyclic nucleotide;
5'"'"'-amino-alkyl phosphate;
1,3-diamino-2-propyl phosphate, 3-aminopropyl phosphate;
6-aminohexyl phosphate;
12-aminododecyl phosphate;
hydroxypropyl phosphate;
1,5-anhydrohexitol nucleotide;
L-nucleotide;
alpha-nucleotide;
threo-pentofuranosyl nucleotide;
acyclic 3'"'"',4'"'"'-seco nucleotide;
3,4-dihydroxybutyl nucleotide;
3,5-dihydroxypentyl nucleotide, 5'"'"'-5'"'"'-inverted abasic moiety;
1,4-butanediol phosphate;
5'"'"'-amino; and
bridging or non bridging methylphosphonate and 5'"'"'-mercapto moieties, and wherein said 3'"'"'-cap structure is selected from the group consisting of 4'"'"'-thio nucleoside, carbocyclic nucleotide;
1,5-anhydrohexitol nucleotide;
L-nucleotide;
alpha-nucleotide;
modified base nucleotide;
threo-pentofuranosyl nucleotide;
acyclic 3'"'"',4'"'"'-seco nucleotide;
3,4-dihydroxybutyl nucleotide;
3,5-dihydroxypentyl nucleotide;
3'"'"'-3'"'"'-inverted abasic moiety;
3'"'"'-2'"'"'-inverted nucleotide moiety;
3'"'"'-2'"'"'-inverted abasic moiety;
1,4-butanediol;
hexylphosphate;
aminohexyl phosphate;
3'"'"'-phosphate; and
a bridging methylphosphonate moiety.
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Abstract
An enzymatic nucleic acid molecule comprising a 5'"'"'- and/or a 3'"'"'-cap structure, wherein said structure is not a 5'"'"'-5'"'"'-linked inverted nucleotide or a 3'"'"'-3'"'"'-linked inverted nucleotide.
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Citations
41 Claims
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1. An enzymatic nucleic acid molecule comprising a 5'"'"'-cap structure, a 3'"'"'-cap structure, or both a 5'"'"'- and a 3'"'"'-cap structure, wherein said 5'"'"'-cap structure is selected from the group consisting of 4'"'"',5'"'"'-methylene nucleotide;
- 1-(β
-D-erythrofuranosyl)nucleotide;
4'"'"'-thio nucleotide, carbocyclic nucleotide;
5'"'"'-amino-alkyl phosphate;
1,3-diamino-2-propyl phosphate, 3-aminopropyl phosphate;
6-aminohexyl phosphate;
12-aminododecyl phosphate;
hydroxypropyl phosphate;
1,5-anhydrohexitol nucleotide;
L-nucleotide;
alpha-nucleotide;
threo-pentofuranosyl nucleotide;
acyclic 3'"'"',4'"'"'-seco nucleotide;
3,4-dihydroxybutyl nucleotide;
3,5-dihydroxypentyl nucleotide, 5'"'"'-5'"'"'-inverted abasic moiety;
1,4-butanediol phosphate;
5'"'"'-amino; and
bridging or non bridging methylphosphonate and 5'"'"'-mercapto moieties, and wherein said 3'"'"'-cap structure is selected from the group consisting of 4'"'"'-thio nucleoside, carbocyclic nucleotide;
1,5-anhydrohexitol nucleotide;
L-nucleotide;
alpha-nucleotide;
modified base nucleotide;
threo-pentofuranosyl nucleotide;
acyclic 3'"'"',4'"'"'-seco nucleotide;
3,4-dihydroxybutyl nucleotide;
3,5-dihydroxypentyl nucleotide;
3'"'"'-3'"'"'-inverted abasic moiety;
3'"'"'-2'"'"'-inverted nucleotide moiety;
3'"'"'-2'"'"'-inverted abasic moiety;
1,4-butanediol;
hexylphosphate;
aminohexyl phosphate;
3'"'"'-phosphate; and
a bridging methylphosphonate moiety. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40)
- 1-(β
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41. A method for producing an enzymatic nucleic acid molecule having activity to cleave an RNA or a single-stranded DNA molecule, comprising the step of forming said enzymatic nucleic acid molecule with a 5'"'"'-cap structure, a 3'"'"'-cap structure, or both a 5'"'"'- and a 3'"'"'-cap structure,
wherein said 5'"'"'-cap structure is selected from the group consisting of 4'"'"',4'"'"'-methylene nucleotide; - 1-(β
-D-erythrofuranosyl)nucleotide;
4'"'"'-thio nucleotide, carbocyclic nucleotide;
5'"'"'-amino-alkyl phosphate;
1,3-diamino-2-propyl phosphate, 3-aminopropyl phosphate;
6-aminohexyl phosphate;
12-aminododecyl phosphate;
hydroxypropyl phosphate;
1,5-anhydrohexitol nucleotide;
L-nucleotide;
alpha-nucleotide;
threo-pentofuranosyl nucleotide;
acyclic 3'"'"',4'"'"'-seco nucleotide;
3,4-dihydroxybutyl nucleotide;
3,5-dihydroxypentyl nucleotide, 5'"'"'-5'"'"'-inverted abasic moiety;
1,4-butanediol phosphate;
5'"'"'-amino; and
bridging or non bridging methylphosphonate and 5'"'"'-mercapto moieties, and wherein said 3'"'"'-cap structure is selected from the group consisting of 4'"'"'-thio nucleoside, carbocyclic nucleotide;
1,5-anhydrohexitol nucleotide;
L-nucleotide;
alpha-nucleotide;
modified base nucleotide;
threo-pentofuranosyl nucleotide;
acyclic 3'"'"',4'"'"'-seco nucleotide;
3,4-dihydroxybutyl nucleotide;
3,5-dihydroxypentyl nucleotide;
3'"'"'-3'"'"'-inverted abasic moiety;
3'"'"'-2'"'"'-inverted nucleotide moiety;
3'"'"'-2'"'"'-inverted abasic moiety;
1,4-butanediol;
hexylphosphate;
aminohexyl phosphate;
3'"'"'-phosphate; and
a bridging methylphosphonate moiety.
- 1-(β
Specification