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Non-instrumented assay with quantitative and qualitative results

  • US 5,998,221 A
  • Filed: 09/25/1996
  • Issued: 12/07/1999
  • Est. Priority Date: 09/25/1996
  • Status: Expired due to Term
First Claim
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1. A process for assaying for an analyte in a sample, said process comprising:

  • providing a solid support having at least two lanes wherein each lane has a contact zone for applying sample to each lane and further wherein the first lane of the solid support has binder immobilized on the entire length of the first lane of the solid support, said binder being a binder for at least the analyte, and the second lane of the solid support is juxtaposed to the first lane of the solid support, and said second lane has binder immobilized on the entire length of said second lane of the solid support, said binder being a binder for at least the analyte, and a known amount of said analyte is immobilized on said binder which is immobilized on the entire length of the second lane of the solid support;

    contacting the contact zone of the first lane with sample and tracer wherein said tracer is comprised of a ligand labeled with a visible label;

    contacting the contact zone of the second lane with tracer; and

    determining the visibility of tracer bound in the first and second lanes of binder as a measure of analyte in the sample.

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