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Magnetically assisted binding assays utilizing a magnetically responsive reagent

  • US 5,998,224 A
  • Filed: 05/16/1997
  • Issued: 12/07/1999
  • Est. Priority Date: 05/16/1997
  • Status: Expired due to Term
First Claim
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1. A method for determining the presence or amount of an analyte in a test sample, said method comprising the steps of:

  • (a) contacting said test sample with both a mobile solid phase reagent and a magnetically responsive reagent to form a reaction mixture, said analyte being capable of specifically binding to both said mobile solid phase reagent and said magnetically responsive reagent to form a complex comprising said mobile solid phase reagent, said magnetically responsive reagent, and said analyte;

    (b) subjecting said reaction mixture to a magnetic field such that a magnetic force is exerted upon said complex if said complex is present in said reaction mixture, the influence of said magnetic force being manifested by the movement or capture of said complex at a different rate from that of said magnetically responsive reagent alone or from that of said mobile solid phase reagent alone; and

    (c) measuring the degree of said manifestation to provide a measure of the presence or amount of said analyte in said test sample.

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