Magnetically assisted binding assays utilizing a magnetically responsive reagent

• US 5,998,224 A
• Filed: 05/16/1997
• Issued: 12/07/1999
• Est. Priority Date: 05/16/1997
• Status: Expired due to Term

• Current Assignee: Abbott Laboratories
• Original Assignee: Abbott Laboratories
• Inventors: Elstrom, Tuan A., Shain, Eric B., Howard, Lawrence V., Rohr, Thomas E.
• Primary Examiner(s): STUCKER, JEFFREY J
• Application Number:
  US08/857,440
• Time in Patent Office: 935 Days
• Field of Search: 436/526, 436/528, 436/534, 436/806, 436/536, 436/538, 422/236, 207/214, 435/7.1
• US Class Current: 436/526
• CPC Class Codes: G01N 33/54333 Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: Y10S 436/806 Electrical property or magnetic property
• CPC Class Codes: Y10S 977/773 Nanoparticle, i.e. structure having three dimensions of 100 nm or less
• CPC Class Codes: Y10S 977/838 Magnetic property of nanomaterial
• CPC Class Codes: Y10S 977/89 Deposition of materials, e.g. coating, cvd, or ald
• CPC Class Codes: Y10S 977/92 Detection of biochemical
• CPC Class Codes: Y10S 977/96 Of magnetic property

Abstract

Assay methods utilizing the response of a magnetically responsive reagent to the influence of a magnetic field to qualitatively or quantitatively measure binding between specific binding pair members. According to the invention, the presence of an analyte mediates whether or not the magnetically responsive reagent binds to a mobile solid phase reagent. The extent of binding will modulate the response of the magnetically responsive reagent or that of the mobile solid phase reagent, or both, to the influence of a magnetic field. Hence, by measuring the response to the magnetic field of the magnetically responsive reagent, or that of the mobile solid phase reagent, the presence or amount of analyte contained in a test sample can accurately be determined. The invention utilizes various devices to carry out the assay methods described.

Claims

1. A method for determining the presence or amount of an analyte in a test sample, said method comprising the steps of:

(a) contacting said test sample with both a mobile solid phase reagent and a magnetically responsive reagent to form a reaction mixture, said analyte being capable of specifically binding to both said mobile solid phase reagent and said magnetically responsive reagent to form a complex comprising said mobile solid phase reagent, said magnetically responsive reagent, and said analyte;

(b) subjecting said reaction mixture to a magnetic field such that a magnetic force is exerted upon said complex if said complex is present in said reaction mixture, the influence of said magnetic force being manifested by the movement or capture of said complex at a different rate from that of said magnetically responsive reagent alone or from that of said mobile solid phase reagent alone; and

(c) measuring the degree of said manifestation to provide a measure of the presence or amount of said analyte in said test sample.

2. The method of claim 1, wherein said test sample is sequentially contacted with said mobile solid phase reagent and said magnetically responsive reagent.

3. The method of claim 1, wherein said test sample is simultaneously contacted with said mobile solid phase reagent and said magnetically responsive reagent.

4. The method of claim 1, wherein the degree of manifestation is determined by detecting an apparent weight change of said attached magnetically responsive reagent in said magnetic field.

5. The method of claim 4, wherein a balance is used to detect said apparent weight change.

6. The method of claim 1, wherein a visual device is used to determine said degree of manifestation.

7. The method of claim 1, wherein an optical device is used to determine said degree of manifestation.

8. The method of claim 1, wherein a position sensing device is used to determine said degree of manifestation.

9. The method of claim 1, wherein said degree of manifestation is determined visually.

10. The method of claim 1, wherein said magnetically responsive reagent comprises superparamagnetic material attached to a specific binding member.

11. The method of claim 10, wherein said superparamagnetic material is a ferrofluid.

12. A method for determining the presence or amount of an analyte in a test sample, said method comprising the steps of:

(a) contacting said test sample with a magnetically responsive reagent and a mobile solid phase reagent to form a reaction mixture, said analyte being capable of specifically binding to said magnetically responsive reagent to form a first complex comprising said analyte and said magnetically responsive reagent and said magnetically responsive reagent being capable of specifically binding to said mobile solid phase reagent to form a second complex comprising said magnetically responsive reagent and said mobile solid phase reagent;

(b) subjecting said reaction mixture to a magnetic field such that a magnetic force is exerted upon said complexes if either of said complexes is present in said reaction mixture, the influence of said magnetic force being manifested by the movement or capture of said second complex at a different rate from that of said magnetically responsive reagent alone or from that of said mobile solid phase reagent alone or said first complex; and

(c) measuring the degree of the manifestation to provide a measure of the presence or amount of said analyte in said test sample.

13. The method of claim 12, wherein said test sample is sequentially contacted with said mobile solid phase reagent and said magnetically responsive reagent.

14. The method of claim 12, wherein said test sample is simultaneously contacted with said mobile solid phase reagent and said magnetically responsive reagent.

15. The method of claim 12, wherein the degree of manifestation is determined by detecting an apparent weight change of said attached magnetically responsive reagent in said magnetic field.

16. The method of claim 15, wherein a balance is used to detect said apparent weight change.

17. The method of claim 12, wherein a visual device is used to determine said degree of manifestation.

18. The method of claim 12, wherein an optical device is used to determine said degree of manifestation.

19. The method of claim 12, wherein a position sensing device is used to determine said degree of manifestation.

20. The method of claim 12, wherein said degree of manifestation is determined visually.

21. The method of claim 12, wherein said magnetically responsive reagent comprises superparamagnetic material attached to a specific binding member.

22. The method of claim 21, wherein said superparamagnetic material is a ferrofluid.

23. A method for determining the presence or amount of an analyte in a test sample, said method comprising the steps of:

(a) contacting said test sample with a magnetically responsive reagent and a mobile solid phase reagent to form a reaction mixture, said analyte being capable of specifically binding to said mobile solid phase reagent to form a first complex comprising said analyte and said mobile solid phase reagent and said magnetically responsive reagent being capable of specifically binding to said mobile solid phase reagent to form a second complex comprising said magnetically responsive reagent and said mobile solid phase reagent;

(b) subjecting said reaction mixture to a magnetic field such that a magnetic force is exerted upon said complexes if either of said complexes is present in said reaction mixture, the influence of said magnetic force being manifested by the movement or capture of said second complex at a different rate from that of said magnetically responsive reagent alone or from that of said mobile solid phase reagent alone or said first complex; and

(c) measuring the degree of the manifestation to provide a measure of the presence or amount of said analyte in said test sample.

24. The method of claim 23, wherein said test sample is sequentially contacted with said mobile solid phase reagent and said magnetically responsive reagent.

25. The method of claim 23, wherein said test sample is simultaneously contacted with said mobile solid phase reagent and said magnetically responsive reagent.

26. The method of claim 23, wherein the degree of manifestation is determined by detecting an apparent weight change of said attached magnetically responsive reagent in said magnetic field.

27. The method of claim 26, wherein a balance is used to detect said apparent weight change.

28. The method of claim 23, wherein a visual device is used to determine said degree of manifestation.

29. The method of claim 23, wherein an optical device is used to determine said degree of manifestation.

30. The method of claim 23, wherein a position sensing device is used to determine said degree of manifestation.

31. The method of claim 23, wherein said degree of manifestation is determined visually.

32. The method of claim 23, wherein said magnetically responsive reagent comprises superparamagnetic material attached to a specific binding member.

33. The method of claim 32, wherein said superparamagnetic material is derived from a ferrofluid.

34. A method for determining the presence or amount of an analyte in a test sample, said method comprising the steps of:

(a) contacting said test sample with at least two different magnetically responsive reagents to form a reaction mixture, said analyte being capable of specifically binding to said at least two of said different magnetically responsive reagents to form a complex comprising said analyte and said at least two different magnetically responsive reagents;

(b) subjecting said reaction mixture to a magnetic field such that a magnetic force is exerted upon said complex, the influence of said magnetic force being manifested by the movement or capture of said complex at a different rate from that of said different magnetically responsive reagents alone; and

(c) measuring the degree of said manifestation to provide a measure of the presence or amount of said analyte in said test sample.

35. The method of claim 34, wherein said test sample is sequentially contacted with said magnetically responsive reagents.

36. The method of claim 34, wherein said test sample is simultaneously contacted with said magnetically responsive reagents.

37. The method of claim 34 wherein the degree of manifestation is determined by detecting an apparent weight change of said attached magnetically responsive reagents in said magnetic field.

38. The method of claim 37, wherein a balance is used to detect said apparent weight change.

39. The method of claim 34, wherein a visual device is used to determine said degree of manifestation.

40. The method of claim 34, wherein an optical device is used to determine said degree of manifestation.

41. The method of claim 34 wherein a position sensing device is used to determine said degree of manifestation.

42. The method of claim 34, wherein said degree of manifestation is determined visually.

43. The method of claim 34, wherein said magnetically responsive reagents comprise superparamagnetic material attached to a specific binding member.

44. The method of claim 43, wherein said superparamagnetic material is a ferrofluid.