Composition for the detection of microorganisms in a sample

• US 5,998,517 A
• Filed: 06/05/1998
• Issued: 12/07/1999
• Est. Priority Date: 06/05/1998
• Status: Expired due to Term

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Gentle, Thomas M. Jr., Yeh, Ming-Hsiung
• Primary Examiner(s): Cain, Edward J.
• Application Number:
  US09/092,689
• Time in Patent Office: 550 Days
• Field of Search: 524/92, 524/176, 523/122
• US Class Current: 524/92
• CPC Class Codes: C09B 57/10 Metal complexes of organic compounds not being dyes in uncomplexed form
• CPC Class Codes: C12Q 1/04 Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor C12Q1/6897 takes precedence

Abstract

The present invention relates to a composition for the detection of the growth of respiring microorganisms in a sample, which comprises:

(a) tris(4,7-diphenyl-10-phenanthroline)ruthenium dichloride pentahydrate;

(b) a hydroxyl functional group;

(c) an organosilicon polymer;

(d) an organohydrogensilicon compound; and

(e) a catalyst;

and a method for preparing said composition.

Claims

1. A composition for detecting the growth of respiring microorganisms in a sample which comprises:

(a) tris (4,7-diphenyl-10-phenanthroline)ruthenium dichloride pentahydrate;

(b) a hydroxyl functional organic;

(c) an organosilicon polymer;

(d) an organohydrogensilicon compound; and

(e) a catalyst.

2. The composition according to claim 1 further comprising a filler.

3. The composition according to claim 2 wherein said filler is fumed silica.

4. The composition according to claim 1 wherein said catalyst is a platinum group metal-containing catalyst.

5. A composition for detecting the growth of respiring microorganisms in a sample which comprises:

(a) tris(4,7-diphenyl-10-phenanthroline)ruthenium dichloride pentahydrate;

(b) a hydroxyl functional silicone compound;

(c) an organosilicon polymer;

(d) an organohydrogensilicon compound; and

(e) a catalyst.

6. The composition according to claim 5 further comprising a filler.

7. The composition according to claim 6 wherein said filler is fumed silica.

8. The composition according to claim 5 further comprising an acid added in an amount sufficient to maintain a neutral pH of about 7.0 of said composition.

9. The composition according to claim 5 wherein said hydroxyl functional silicone compound has the formula X_n R.sub.(3-n) SiO(RXSiO)_m SiR.sub.(3-n) X_n wherein

R independently represents a C₁ -C₂₀ hydrocarbon radical;

X independently represents a hydroxyl radical or an R¹ radical;

n is the number 1 or 2;

m is a number sufficient to provide a viscosity of about 1 to about 500 millipascal-seconds at a viscosity of 25°

C.; and

R¹ is an unsaturated aliphatic hydrocarbon optionally substituted with 1 to 20 carbon atoms.

10. The composition according to claim 5 wherein said catalyst is a platinum group metal-containing catalyst.

11. A method for preparing a composition for detecting the growth of respiring microorganisms in a sample which comprises the steps of:

(i) forming a solution of tris(4,7-diphenyl-10-phenanthroline)ruthenium dichloride pentahydrate and a hydroxyl functional group containing compound;

(ii) adding an organosilicon polymer to said solution forming a mixture;

(iii) adding an organohydrogensilicon compound to said mixture in the presence of a catalyst forming an elastomer; and

(iv) adding a filler to said elastomer.

12. The method of claim 11 wherein said hydroxyl functional group containing compound is a hydroxyl functional organic.

13. The method of claim 12 wherein said filler is fumed silica.

14. The method of claim 13 wherein said catalyst is a platinum group metal-containing catalyst.

15. The method of claim 11 wherein said hydroxyl functional group containing compound is a hydroxyl functional silicone compound.

16. The method of claim 15 wherein said filler is funed silica.

17. The method of claim 16 further comprising an acid added in an amount sufficient to maintain a neutral pH of about 7.0 of said composition.

18. The method of claim 17 wherein said hydroxyl functional silicone compound has the formula X_n R.sub.(3-n) SiO(RXSiO)_m SiR.sub.(3-n) X_n wherein

R independently represents a C₁ -C₂₀ hydrocarbon radical;

X independently represents a hydroxyl radical or an R¹ radical;

n is the number 1 or 2;

m is a number sufficient to provide a viscosity of about 1 to about 500 millipascal-seconds at a viscosity of 25°

C.; and

R¹ is an unsaturated aliphatic hydrocarbon optionally substituted with 1 to 20 carbon atoms.

19. The method of claim 18 wherein said catalyst is a platinum group metal-containing catalyst.

20. A composition for detecting the growth of respiring microorganisms in a sample wherein said composition comprises tris-(4,7-diphenyl-10-phenanthroline)ruthenium dichloride pentahydrate dissolved in a silanol functional silicon polymer forming a solution and wherein said solution is incorporated into a liquid silicone polymer.