Resurfacing cartilage defects with chondrocytes proliferated without differentiation using platelet-derived growth factor

• US 6,001,352 A
• Filed: 03/31/1997
• Issued: 12/14/1999
• Est. Priority Date: 03/31/1997
• Status: Expired due to Term

• Current Assignee: Board of Regents of the University of Texas System (University of Texas System)
• Original Assignee: OsteoBiologics Inc. (Smith & Nephew PLC)
• Inventors: Schwartz, Zvi, Boyan, Barbara D.
• Primary Examiner(s): Naff, David M.
• Application Number:
  US08/829,308
• Time in Patent Office: 988 Days
• Field of Search: 424/93.7, 424/85.1, 424/423, 424/426, 424/548, 435/177, 435/180, 435/182, 435/384, 435/395, 435/405, 435/325, 435/366, 435/375, 435/377, 435/396
• US Class Current: 424/93.7
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: C12N 2501/135 Platelet-derived growth factor [PDGF]
• CPC Class Codes: C12N 5/0655 Chondrocytes; Cartilage

Abstract

A method for stimulating chondrocyte proliferation and inhibiting chondrocyte differentiation along the endochondral developmental pathway is provided comprising contacting condrocytes with an effective amount of Platelet-Derived Growth Factor (PDGF) such as PDGF-BB, PDGF-AA OR PDGF-AB in the substantial absence of growth factors which promote cell differentiation. This allows such cells to be multiplied in culture for loading onto a scaffolding material and implanting into a cartilage or bone wound.

Claims

1. A method for resurfacing an articular cartilage defect in a patient comprising:

(a) isolating chondrocyte cells from the patient;

(b) contacting said cells with a Platelet-Derived Growth Factor (PDGF) selected from the group consisting of PDGF-BB, PDGF-AA and PDGF AB for about 30 minutes to about 24 hours in an amount effective to inhibit chondrocyte endochondral maturation to hypertrophic and in the substantial absence of growth factors which promote the hypertrophic stage of cell differentiation;

(c) loading said cells onto a cell scaffolding material; and

(d) placing said cell scaffolding material in said defect.

2. The method of claim 1 wherein said scaffolding material is biodegradable.