Attenuated strain of the virus causing the porcine reproductive respiratory syndrome (PRRS), and vaccines

• US 6,001,370 A
• Filed: 11/24/1997
• Issued: 12/14/1999
• Est. Priority Date: 01/25/1996
• Status: Expired due to Fees

• Current Assignee: Laboratorios HIPRA, S.A.
• Original Assignee: Laboratories Cinfa SA (Infarco SA)
• Inventors: Maso, Enric Espuna, Burch, Reina Alemany, Roca, Narcis Saubi, Pujadas, Pere Riera
• Primary Examiner(s): Mosher, Mary E.
• Assistant Examiner(s): SALIMI, ALI REZA
• Application Number:
  US08/930,330
• Time in Patent Office: 750 Days
• Field of Search: 424/184.1, 424/201.1, 424/204.1, 424/209.1, 424/218.1, 424/815, 424/202.1, 435/235.1, 435/236, 435/237, 435/239
• US Class Current: 424/204.1
• CPC Class Codes: A61K 2039/5254 avirulent or attenuated
• CPC Class Codes: A61K 2039/552 Veterinary vaccine
• CPC Class Codes: A61K 39/12 Viral antigens
• CPC Class Codes: A61P 31/12 Antivirals
• CPC Class Codes: A61P 31/14 for RNA viruses
• CPC Class Codes: C12N 2770/10021 Viruses as such, e.g. new isolates, mutants or their genomic sequences
• CPC Class Codes: C12N 2770/10034 Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
• CPC Class Codes: C12N 2770/10061 Methods of inactivation or attenuation
• CPC Class Codes: C12N 7/00 Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00
• CPC Class Codes: G01N 33/56983 Viruses
• CPC Class Codes: Y10S 424/815 Viral vaccine for porcine species, e.g. swine

Abstract

A new attenuated strain (CNCM Institut Pasteur I-1642) of the virus causing the swine disease known as porcine reproductive and respiratory syndrome (PRRS) is described, along with an attenuation and replication procedure of the same by using a new clone obtained from MA-104 monkey kidney cells (CNCM Institut Pasteur I-1643). Because of the innocuousness on swine and the high immunogenic activity, the said new attenuated strain allows the obtaining of vaccines and diagnostic kits which make possible both the early diagnosis of PRRS and an efficient preventive treatment of such disease.

Claims

1. A vaccine to protect swine against the disease known as porcine reproductive and respiratory syndrome (PRRS) containing the attenuated viral strain that corresponds to the deposit on Nov. 11, 1995 with CNCM of Institute Pasteur, having access number I-1642.

2. A vaccine according to claim 1 characterized by containing between 10² and 10⁶ TCID₅₀ of the attenuated viral strain.

3. A vaccine according to claim 1, wherein the vaccine dose contains the attenuated viral strain or inactivate virus.

4. A vaccine according to claim 1, containing additional immunostimulating adjuvants and/or emulsifiers and/or stabilizers.

5. A vaccine according to claim 1, containing additional swine viruses either live or inactivated, in either single or combined form.

6. A vaccine according to claim 1 containing additional live or inactivated bacteria.