DNA polymerase having 3'-intrinsic editing activity

• US 6,001,566 A
• Filed: 05/31/1996
• Issued: 12/14/1999
• Est. Priority Date: 06/01/1995
• Status: Expired due to Term

• Current Assignee: Roche Diagnostics GmbH (Roche Holding AG)
• Original Assignee: Roche Diagnostics GmbH (Roche Holding AG)
• Inventors: Canard, Bruno, Sagner, Gregor
• Primary Examiner(s): Degen, Nancy
• Assistant Examiner(s): WANG, ANDREW J
• Application Number:
  US08/655,777
• Time in Patent Office: 1,292 Days
• Field of Search: 435/91.1, 435/6, 435/91.5, 435/183, 436/94, 536/23.1
• US Class Current: 435/6.12
• CPC Class Codes: C12Q 1/48 involving transferase
• CPC Class Codes: C12Q 1/6869 Methods for sequencing

Abstract

The invention is directed to the use of RNA or DNA polymerases having 3'"'"'-intrinsic editing activity (3'"'"'-IEA) in the presence or absence of a deactivating agent to remove a protecting group from the 3'"'"'-position of oligo- polyribo- or deoxyribonnucleotides. The use is connected with the incorporation of dNTPs into DNA templates in order to determine the concentration and/or sequence of said templates. In particular the use is concerned with an improved non gel-based sequencing method.

Claims

1. A method for determining the incorporation of at least one tagged dNTP into a double-stranded DNA product, comprising:

(a) providing, in a reaction vessel, a reaction solution comprising

(i) a DNA template,

(ii) at least one primer,

(iii) a plurality of nucleotides comprising a plurality of dATP nucleotides, dTTP nucleotides, dCTP nucleotides and dGTP nucleotides, or analogues thereof, and at least one tagged dNTP of formula I, or an analogue thereof ##STR2## wherein;

X is a bifunctional linkage group,

Y is an activated group,

B is selected from the group consisting of a purine, a pyrimidine, a deazapurine and a deazapyrimidine, and

(iv) a thermophilic DNA polymerase which exhibits 3'"'"'-intrinsic editing activity;

(b) synthesizing, in the reaction solution, a double-stranded DNA product in a polymerase chain reaction by sequentially adding to the at least one primer a plurality of nucleotides from step (a) which are complementary to the DNA template, the synthesizing step including incorporating the at least one tagged dNTP into the double-stranded DNA product and releasing the activated group from the at least one tagged dNTP concomitant to attaching to the at least one tagged dNTP one of the plurality of nucleotides from step (a) which is complementary to the DNA template, wherein said releasing step is effected by the DNA polymerase and is not effected by an additional enzyme, by radiation, or by an additional chemical; and

(c) detecting the released activated group.

2. The method of claim 1, wherein X is selected from the group consisting of oxygen, sulfur and a --NH-group.

3. The method of claim 1, wherein Y is selected from the group consisting of --C(O)R, --CH₂ --R, --C(S)NH--R and --C(O)NH--R, wherein R is selected from the group consisting of a hapten, a dye, a chromophore and a branched or unbranched alkyl group.

4. The method of claim 3, wherein R is at least one of a fluorescent chromophore and a C₁ -C₂₀ alkyl group.

27. The method of claim 1, wherein the reaction solution is buffered.

5. A method of determining an amount of tagged dNTPs incorporated into a double-stranded DNA product, comprising:

(a) providing, in a reaction vessel, a reaction solution comprising

(i) a DNA template,

(ii) at least one primer,

(iii) a plurality of nucleotides comprising (1) three different non-tagged dNTPs, or analogues thereof, wherein each of the three different non-tagged dNTPs or an analogue thereof comprises a different one of the four DNA nucleotide bases, and (2) a plurality of tagged dNTPs of formula I, or analogues thereof, ##STR3## wherein;

X is a bifunctional linkage group,

Y is an activated group,

B is selected from the group consisting of a purine, a pyrimidine, a deazapurine and a deazapyrimidine, wherein B is a DNA nucleotide base which is different from each of the three different non-tagged dNTPs or an analogue thereof,

(iv) a thermophilic DNA polymerase which exhibits 3'"'"'-intrinsic editing activity; and

(v) a suitable buffer;

(b) synthesizing, in the reaction solution, a double-stranded DNA product in a polymerase chain reaction by sequentially adding to the at least one primer a plurality of nucleotides from step (a) which are complementary to the DNA template, the synthesizing step including incorporating a plurality of tagged dNTPs into the double-stranded DNA product and releasing the activated group from each of the plurality of tagged dNTPs concomitant to attaching to each of the incorporated tagged dNTPs one of the plurality of nucleotides from step (a) which is complementary to the DNA template, wherein said releasing step is effected by the DNA polymerase and is not effected by an additional enzyme, by radiation, or by an additional chemical;

(c) determining the concentration of the released activated group in the reaction solution; and

(d) relating the concentration of the released activated group to the amount of tagged dNTPs incorporated into the double-stranded DNA product.

6. The method of claim 5, wherein X is selected from the group consisting of oxygen, sulfur and a --NH-group.

7. The method of claim 5, wherein Y is selected from the group consisting of --C(O)R, --CH₂ --R, --C(S)NH--R and --C(O)NH--R, wherein R is selected from the group consisting of a hapten, a dye, a chromophore and a branched or unbranched alkyl group.

8. The method of claim 7, wherein R is at least one of a fluorescent chromophore and a C₁ -C₂₀ alkyl group.

9. A method of calculating an amount of repeating sequences in a repeating region of a DNA sequence, wherein each repeating sequence comprises one, two or three of the four DNA nucleotide bases, the method comprising:

(a) hybridizing a primer with a DNA sequence comprising a repeating region comprising a plurality of repeating sequences, wherein each repeating sequence comprises one, two or three of the four DNA nucleotide bases to produce a primed DNA, wherein the primer flanks the repeating region;

(b) in a reaction vessel containing a reaction solution, combining the primed DNA with a plurality of nucleotides comprising

(i) a first non-tagged dNTP, or analogue thereof,

(ii) a second non-tagged dNTP, or analogue thereof,

(iii) a non-tagged ddNTP, or analogue thereof,

(iv) a tagged dNTP of formula I, or analogue thereof, ##STR4## wherein;

X is a bifunctional linkage group,

Y is an activated group, and

B is selected from the group consisting of a purine, a pyrimidine, a deazapurine and a deazapyrimidine,

wherein each of (i), (ii), (iii) and (iv) comprises a different one of the four DNA nucleotide bases, and wherein the non-tagged ddNTP or analogue thereof is a DNA nucleotide base which is non-complementary to a DNA nucleotide base of the repeating sequence and the tagged dNTP or analogue thereof is complementary to a DNA nucleotide base of the repeating sequence, and

(v) a thermophilic DNA polymerase which exhibits 3'"'"'-intrinsic editing activity;

(c) synthesizing, in the reaction solution, a double-stranded DNA product in a polymerase chain reaction by sequentially adding to the primer a plurality of nucleotides from step (b) which are complementary to the DNA sequence, the synthesizing step including incorporating a plurality of tagged dNTPs into the double-stranded DNA product and releasing the activated group from each of the plurality of tagged dNTPs concomitant to attaching to each of the incorporated tagged dNTPs one of the plurality of nucleotides from step (a) which is complementary to the DNA sequence wherein said releasing step is effected by the DNA polymerase and is not effected by an additional enzyme, by radiation, or by an additional chemical;

(d) terminating the synthesis of the double-stranded DNA product outside of the repeating region by incorporating the non-tagged ddNTP or analogue thereof into the double-stranded DNA product;

(e) determining the concentration of the released activated group in the reaction solution; and

(f) relating the concentration of the released activated group to the amount of repeating sequences in the repeating region of the DNA sequence.

10. The method of claim 9, wherein X is selected from the group consisting of oxygen, sulfur and a --NH-group.

11. The method of claim 9, wherein Y is selected from the group consisting of --C(O)R, --CH₂ --R, --C(S)NH--R and --C(O)NH--R, wherein R is selected from the group consisting of a hapten, a dye, a chromophore and a branched or unbranched alkyl group.

12. The method of claim 11, wherein R is at least one of a fluorescent chromophore and a C₁ -C₂₀ alkyl group.

13. A method of determining a nucleotide sequence of a DNA sample, comprising:

(a) hybridizing a primer with the DNA sample to produce a primed DNA, wherein the primer flanks the repeating region;

(b) attaching to the primed DNA a first tagged dNTP of formula I, or an analogue thereof, in the presence of a thermophilic DNA polymerase, which exhibits 3'"'"'-intrinsic editing activity, in a reaction vessel containing a reaction solution, wherein the first tagged dNTP is complementary to the DNA sample, to form a double-stranded DNA product, ##STR5## wherein;

X is a bifunctional linkage group,

Y is an activated group, and

B comprises one of the four DNA nucleotide bases;

(c) determining a nucleotide in the nucleotide sequence of the DNA sample by detecting the presence or absence of the activated group of the first tagged dNTP on the double-stranded DNA product and in the reaction solution;

(d) releasing the activated group of the first tagged dNTP by repeating, at least once, step (b) using a second tagged dNTP of formula I, or an analogue thereof, which is complementary to the DNA sample, wherein the activated group of the first tagged dNTP is released from the first tagged dNTP concomitant to attaching to the primed DNA the second tagged dNTP or analogue thereof, wherein said releasing step is effected by the DNA polymerase and is not effected by an additional enzyme, by radiation, or by an additional chemical; and

(e) determining the nucleotide sequence of the DNA sample, by detecting the presence or absence of the activated group of the first tagged dNTP and the activated group of the second tagged dNTP on the double-stranded DNA product and in the reaction solution.

14. The method of claim 13, wherein X is selected from the group consisting of oxygen, sulfur and a --NH-group.

15. The method of claim 13, wherein Y is selected from the group consisting of --C(O)R, --CH₂ --R, --C(S)NH--R and --C(O)NH--R, wherein R is selected from the group consisting of a hapten, a dye, a chromophore and a branched or unbranched alkyl group.

16. The method of claim 15, wherein R is at least one of a fluorescent chromophore and a C₁ -C₂₀ alkyl group.

17. A method of determining a nucleotide sequence of each of a plurality of DNA samples, the method comprising:

(a) providing a plurality of DNA samples immobilized on a solid support, wherein each DNA sample comprises a first strand having a nucleotide region which nucleotide sequence is to be determined and a second strand, having a primed end, flanking the nucleotide region;

(b) providing a plurality of nucleotides comprising

(i) a first tagged dNTP of formula I, or an analogue thereof,

(ii) a second tagged dNTP of formula I, or an analogue thereof,

(iii) a third tagged dNTP of formula I, or an analogue thereof, and

(iv) a fourth tagged dNTP of formula I, or an analogue thereof, ##STR6## wherein;

X is a bifunctional linkage group,

Y is an activated group, and

B is selected from the group consisting of a purine, a pyrimidine, a deazapurine and a deazapyrimidine,

wherein each of (i), (ii), (iii) and (iv) comprises a different one of the four DNA nucleotide bases;

(c) attaching the first tagged dNTP or analogue thereof to the second strand of each DNA sample having a complementary nucleotide therewith on the first strand by combining, in a reaction vessel, the first tagged dNTP or analogue thereof, the plurality of DNA samples and a thermophilic DNA polymerase, wherein said attaching step is conducted at a temperature below the optimum temperature for the DNA polymerase activity;

(d) determining a nucleotide in the nucleotide sequence of each of the plurality of DNA samples to which the first tagged dNTP or analogue thereof has been attached in step (c), by detecting the attached activated group of the attached first tagged dNTP or analogue thereof,

(e) repeating steps (c)-(d) with the second tagged dNTP or analogue thereof,

(f) repeating steps (c)-(d) with the third tagged dNTP or analogue thereof,

(g) repeating steps (c)-(d) with the fourth tagged dNTP or analogue thereof,

(h) removing each of the activated groups on (i), (ii), (iii) and (iv), and

(i) repeating, at least once, steps (c)-(h) to determine the nucleotide sequence of each of the plurality of DNA samples.

18. The method of claim 17, wherein X is selected from the group consisting of oxygen, sulfur and a --NH-group.

19. The method of claim 17, wherein Y is selected from the group consisting of --C(O)R, --CH₂ --R, --C(S)NH--R and --C(O)NH--R, wherein R is selected from the group consisting of a hapten, a dye, a chromophore and a branched or unbranched alkyl group.

20. The method of claim 19, wherein R is at least one of a fluorescent chromophore and a C₁ -C₂₀ alkyl group.

21. The method of claim 17, wherein the activating groups are removed using a ribo- or desoxyribonucleotide-5'"'"'-triphosphate, or a derivative thereof.

22. A method of determining a nucleotide of a DNA sample, the method comprising:

(a) providing a DNA sample immobilized on a solid support, wherein the DNA sample comprises a first strand having a nucleotide which is to be determined and a second strand, having a primed end, flanking the nucleotide;

(b) providing a plurality of nucleotides comprising

(i) a tagged dATP nucleotide of formula I, or an analogue thereof,

(ii) a tagged dTTP nucleotide of formula I, or an analogue thereof,

(iii) a tagged dCTP nucleotide of formula I, or an analogue thereof, and

(iv) a tagged dGTP nucleotide of formula I, or an analogue thereof, ##STR7## wherein;

X is a bifunctional linkage group,

Y is an activated group,

B is selected from the group consisting of a purine, a pyrimidine, a deazapurine and a deazapyrimidine,

wherein each of (i), (ii), (iii) and (iv) comprises a different activated group;

(c) attaching one of (i), (ii), (iii) and (iv), having a complementary nucleotide to the nucleotide which is to be determined, to the second strand of the DNA sample to produce a tagged DNA sample by combining, in a reaction vessel, the plurality of nucleotides, the DNA sample and a thermophilic DNA polymerase, wherein said attaching step is conducted at a temperature below the optimum temperature for the DNA polymerase activity; and

(d) determining the nucleotide of the tagged DNA sample by detecting the attached activated group of the attached tagged nucleotide or analogue thereof.

23. The method of claim 22, wherein X is selected from the group consisting of oxygen, sulfur and a --NH-group.

24. The method of claim 22, wherein Y is selected from the group consisting of --C(O)R, --CH₂ --R, --C(S)NH--R and --C(O)NH--R, wherein R is selected from the group consisting of a hapten, a dye, a chromophore and a branched or unbranched alkyl group.

25. The method of claim 24, wherein R is at least one of a fluorescent chromophore and a C₁ -C₂₀ alkyl group.

26. The method of claim 22, further comprising, after step (c), separating the tagged DNA sample and the plurality of nucleotides.