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Compositions, methods, kits and apparatus for determining the presence or absence of target molecules

  • US 6,001,570 A
  • Filed: 02/18/1997
  • Issued: 12/14/1999
  • Est. Priority Date: 02/18/1997
  • Status: Expired due to Fees
First Claim
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1. A first ribonucleic acid (RNA) molecule and a second RNA molecule for use in determining the presence or absence of a target molecule, said first RNA molecule capable of binding to a target molecule and having the following formula:

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    space="preserve" listing-type="equation">5'"'"'-A-B-C-3'"'"';

    wherein A is a section of the RNA molecule having 10-100,000 nucleotides which section is capable of being received by an RNA replicase and with another RNA sequence, F, being replicated and the letter "B" denotes a section of the RNA molecule having approximately 10 to 50,000 nucleotides which section is capable of binding to the target molecule and the letter "C" denotes a section of the RNA molecule having approximately 1 to 10,000 nucleotides which section is capable of being ligated to another RNA sequence, "D";

    said second RNA molecule capable of binding to said target molecule and having the following formula;

    
    
    space="preserve" listing-type="equation">5'"'"'-D-E-F-3'"'"';

    wherein D is a section of the RNA molecule having approximately 1 to 10,000 nucleotides which section is capable being ligated with another RNA sequence, "C", and the letter "E" denotes a section of the RNA molecule having approximately 10 to 100,000 nucleotides which section is capable of binding to the target molecule, and the letter "F" denotes a section of the RNA molecule having 10 to 100,000 nucleotides which section is capable of being received by an RNA replicase and with another sequence, "A", being replicated;

    said first and the second RNA molecules are capable of forming a third RNA molecule having the following formula;

    
    
    space="preserve" listing-type="equation">5'"'"'-A-B-C-D-E-F-3'"'"';

    said third RNA molecule formed by ligating the C and D sections, as the E and the B sections are bound to target, said third RNA molecule capable of being received by an RNA replicase and being replicated by such enzyme as a indication of the presence or absence ol the target molecule.

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