Modified nucleic acid amplification primers
First Claim
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1. An oligonucleotide primer for the amplification of a nucleic acid sequence, said primer having the general structure:
- ##STR10## wherein S1 represents a first sequence of nucleotides between about 5 and about 50 nucleotides in length;
wherein S2 represents a second sequence between one and three nucleotides in length;
wherein N represents a nucleotide that contains a purine or pyrimidine base that contains an exocyclic amine;
wherein R represents a modifier group, wherein R is covalently bound to the nitrogen atom of the exocyclic amine, and wherein R has the structure;
##STR11## wherein R1 and R2 represent independently hydrogen, a C1 -C10 alkyl group, an alkoxy group, a phenyl group, a phenoxy group, a substituted phenyl group, a napthyl group, or a substituted napthyl group.
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Abstract
The present invention provides modified primers for use in the amplification of a nucleic acid sequence. Amplifications carried out using the modified primers result in less non-specific amplification product, in particular, primer dimer, and a concomitant greater yield of the intended amplification product compared to amplifications carried out using unmodified primers.
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Citations
22 Claims
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1. An oligonucleotide primer for the amplification of a nucleic acid sequence, said primer having the general structure:
- ##STR10## wherein S1 represents a first sequence of nucleotides between about 5 and about 50 nucleotides in length;
wherein S2 represents a second sequence between one and three nucleotides in length; wherein N represents a nucleotide that contains a purine or pyrimidine base that contains an exocyclic amine; wherein R represents a modifier group, wherein R is covalently bound to the nitrogen atom of the exocyclic amine, and wherein R has the structure;
##STR11## wherein R1 and R2 represent independently hydrogen, a C1 -C10 alkyl group, an alkoxy group, a phenyl group, a phenoxy group, a substituted phenyl group, a napthyl group, or a substituted napthyl group. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
- ##STR10## wherein S1 represents a first sequence of nucleotides between about 5 and about 50 nucleotides in length;
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15. A method for amplifying a nucleic acid target sequence, wherein said method comprises carrying out an amplification reaction using oligonucleotide primers, wherein at least one primer has the structure:
- ##STR13## wherein S1 represents a first sequence of nucleotides between about 5 and about 50 nucleotides in length;
wherein S2 represents a second sequence between one and three nucleotides in length; wherein N represents a nucleotide that contains a purine or pyrimidine base that contains an exocyclic amine; wherein R represents a modifier group, wherein R is covalently bound to the nitrogen atom of the exocyclic amine, and wherein R has the structure;
##STR14## wherein R1 and R2 represent independently hydrogen, a C1 -C10 alkyl group, an alkoxy group, phenyl group, a phenoxy group, a substituted phenyl group, a napthyl group, or a substituted napthyl group. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22)
- ##STR13## wherein S1 represents a first sequence of nucleotides between about 5 and about 50 nucleotides in length;
Specification