System for manipulating drops and bubbles using acoustic radiation pressure

• US 6,003,388 A
• Filed: 09/17/1997
• Issued: 12/21/1999
• Est. Priority Date: 09/17/1997
• Status: Expired due to Fees

• Current Assignee: US Government As Represented By The Director National Security Agency
• Original Assignee: United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration
• Inventors: Oeftering, Richard C.
• Primary Examiner(s): Williams, Hezron
• Assistant Examiner(s): MILLER, ROSE MARY
• Application Number:
  US08/969,536
• Time in Patent Office: 825 Days
• Field of Search: 210/748, 210/738, 210/188, 209/155, 209/163, 406/198, 367/137, 367/138, 367/191, 367/99, 422/99, 422/100, 73/570, 73/432.1, 73/61.75, 73/64.53, 73/865.5, 73/627, 73/864.01, 73/864.14, 73/864.73, 73/597, 310/328, 310/334, 347/47, 347/48, 347/68, 347/70, 222/420
• US Class Current: 73/864.01
• CPC Class Codes: B01L 2400/0436 acoustic forces, e.g. surface acoustic waves [SAW]
• CPC Class Codes: B01L 3/0241 Drop counters; Drop formers making arrays for combinatorial libraries B01J19/0046; automation of dispensing for analysis G01N35/10
• CPC Class Codes: G01N 13/02 Investigating surface tension of liquids
• CPC Class Codes: G01N 2013/0241 bubble, pendant drop, sessile drop methods
• CPC Class Codes: G01N 2035/1046 Levitated, suspended drops

Abstract

The manipulation and control of drops of liquid and gas bubbles is achieved using high intensity acoustics in the form of and/or acoustic radiation pressure and acoustic streaming, generated by a controlled wave emission from a transducer. Acoustic radiation pressure is used to deploy or dispense drops into a liquid or a gas or bubbles into a liquid at zero or near zero velocity from the discharge end of a needle such as a syringe needle. Acoustic streaming is useful in manipulating the drop or bubble during or after deployment. Deployment and discharge is achieved by focusing the acoustic radiation pressure on the discharge end of the needle, and passing the acoustic waves through the fluid in the needle, through the needle wall itself, or coaxially through the fluid medium surrounding the needle. Alternatively, the acoustic waves can be counter-deployed by focusing on the discharge end of the needle from a transducer axially aligned with the needle, but at a position opposite the needle, to prevent premature deployment of the drop or bubble. The acoustic radiation pressure can also be used for detecting the presence or absence of a drop or a bubble at the tip of a needle or for sensing various physical characteristics of the drop or bubble such as size or density.

Claims

1. A method for manipulating a drop of a first fluid at the discharge end of a conduit comprising:

a) positioning the drop of the first fluid at the discharge end, in the presence of a second fluid

b) focusing a source of acoustical radiation pressure on the drop, and

c) causing the acoustical radiation pressure to change the relationship between the drop and the discharge end of the conduit.

2. The method according to claim 1 wherein the drop is a liquid and the second fluid is selected from the group consisting of a liquid, a gas and a vacuum.

3. The method according to claim 1 wherein the drop is a gas bubble, and the second fluid is a liquid.

4. The method according to claim 1 wherein the manipulation includes the use of acoustical radiation pressure to dislodge the drop from the discharge end at a near zero velocity.

5. The method according to claim 1 wherein the manipulation further includes acoustical streaming to assist in changing the relationship between the drop and the discharge end of the conduit.

6. The method according to claim 1 wherein the source of acoustical radiation pressure is a piezoelectric transducer.

7. The method according to claim 6 wherein the conduit is filled with the first fluid and the acoustical radiation pressure is transmitted to the discharge end of the conduit through the first fluid in the conduit.

8. The method according to claim 6 wherein the acoustic radiation pressure is generated in a transducer positioned at a location remote from the conduit and in axial alignment therewith, and passes through the second fluid to the drop.

9. The method according to claim 8 wherein the acoustic radiation pressure controls the drop following dispensing from the discharge end of the conduit.

10. The method according to claim 6 wherein the piezoelectric transducer is focused on the drop at the discharge end of the conduit, and the acoustic radiation pressure is transmitted to the drop through the second fluid surrounding the conduit.

11. The method according to claim 10 wherein the piezoelectric transducer is annular.

12. The method according to claim 1 wherein the drop is manipulated in a zero gravity environment.

13. A method according to claim 1 wherein the drop is discharged from the conduit at a low discharge velocity by empirically using acoustic radiation pressure bursts of increasing duration until surface tension between the discharge end of the conduit and the drop is exceeded.

14. The method according to claim 1 wherein drops of differing sizes are dispensed from the discharge end of the conduit by the use of acoustic radiation pressure bursts of differing durations.

15. The method according to claim 1 wherein pulsing of the acoustical radiation pressure is used to cause the drop to oscillate.

16. The method according to claim 15 wherein oscillation of the drop causes the drop to dislodge from the discharge end of the conduit.

17. The method according to claim 16 wherein the oscillation of the drop is analyzed to determine the physical properties thereof.

18. The method according to claim 1 wherein the acoustic radiation pressure is used to suppress externally induced oscillations within the drop.

19. The method according to claim 1 including the further step of using acoustic radiation pressure to sense a physical property of the drop.

20. An apparatus for deploying drops of first fluid into a second fluid, the apparatus comprising a tubular needle to conduct the first fluid, said needle comprising a fluid dispensing end and a fluid coupling end;

an acoustic transducer;

an acoustic housing to support the transducer and to provide containment for the first fluid, the housing in fluid communication with the coupling end of the needle;

electrical connection between the acoustic transducer and an electrical signal, means to transmit acoustic energy from the transducer to the dispensing end of the needle, and means to generate the electrical signal to cause the focused acoustical energy to deploy the drops.

21. The apparatus according to claim 20 wherein the electrical signal originates in a signal generator circuit.

22. The apparatus according to claim 21 wherein the signal generator circuit includes a radio frequency power amplifier circuit.

23. The apparatus according to claim 22 wherein the radio frequency power amplifier circuit includes a network to match the impedance of the amplifier circuit with that of the transducer.

24. The apparatus according to claim 20 further including an acoustic feedback signal for purposes of acoustic sensing.

25. The apparatus according to claim 20 wherein the needle includes a bore which has a smooth inner diameter and the transducer is adapted to be acoustically coupled to the fluid in the bore.

26. The apparatus according to claim 20 wherein the transducer is acoustically coupled to the coupling end of the tubular needle, and includes a tapered internal bore which tapers from a larger bore at the coupling end to a smaller internal bore toward the dispensing end.

27. The apparatus according to claim 20 wherein the transducer is adapted to be acoustically coupled to the second fluid surrounding the needle, and to be focused at the dispensing end of the needle.

28. A method for manipulating a drop of a first fluid at the discharge end of a conduit comprising:

a) positioning said drop of first fluid at the discharge end in the presence of a second fluid;

b) generating acoustic radiation pressure in a transducer positioned at a location remote from the conduit and in axial alignment therewith;

c) focusing a source of acoustical radiation pressure on the drop; and

d) passing the acoustic radiation pressure through the second fluid to prevent premature dispensing of the drop.

29. A process for using acoustic radiation pressure for sensing, the process comprising:

a) focusing an acoustic radiation transducer on the discharge end of a needle containing a first fluid, said discharge end surrounded by a second fluid;

b) emitting a burst of acoustic radiation of finite duration from the transducer;

c) gathering reflected acoustic waves from the target; and

d) analyzing the reflected acoustic waves to determine the presence or absence of a drop of the first fluid at the discharge end of the needle.

30. An apparatus for manipulating drops of a first fluid in the presence of a second fluid, the apparatus comprising a tubular needle to conduct the first fluid, said needle comprising a fluid coupling end and a fluid dispensing end;

an acoustic transducer positioned at a location remote from the needle and focused at the dispensing end thereof, an acoustic housing to support the transducer;

electrical connection between the acoustic transducer and an electrical signal, and means to transmit acoustic energy from the transducer through the second fluid to the fluid dispensing end of the needle.

31. The apparatus according to claim 30 wherein the acoustic transducer is in axial alignment with the fluid dispensing end of the needle.

32. The apparatus according to claim 31 including a second acoustical transducer coaxially surrounding the fluid dispensing end of the needle and focused at the dispensing end thereof, an acoustic housing to support the second transducer, and means to generate an electrical signal to transmit acoustic energy through the second transducer to the dispensing end of the needle in an axial direction opposed to the direction of the acoustical energy of the first transducer.