DNA Sequencing

• US 6,004,446 A
• Filed: 02/18/1998
• Issued: 12/21/1999
• Est. Priority Date: 03/29/1984
• Status: Expired due to Fees

• Current Assignee: Li & S Company Limited (Yesco Holdings Co., Ltd.)
• Original Assignee: Li & S Company Limited (Yesco Holdings Co., Ltd.)
• Inventors: Eckles, Robert David, Bruce, Robert Clark, Middendorf, Lyle Richard
• Primary Examiner(s): Till, Terrence R.
• Assistant Examiner(s): STARSIAK, JOHN S
• Application Number:
  US09/025,885
• Time in Patent Office: 671 Days
• Field of Search: 204/612, 204/618, 204/467, 204/461, 204/621, 204/616, 204/466
• US Class Current: 204/618
• CPC Class Codes: C12Q 1/6869 Methods for sequencing
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 27/44704 Details; Accessories
• CPC Class Codes: G01N 27/44721 by optical means
• CPC Class Codes: G01N 27/44726 using specific dyes, markers or binding molecules

Abstract

To sequence DNA, DNA samples marked with flourscent infrared dye are applied at a plurality of locations for electrophoresing in a plurality of channels through a gel electrophoresis slab. The channels are scanned with a laser and a sensor, that include a microscope focused on the gel slab. The focal point and slab are adjusted with respect to each other so that the focal point of the microscope remains on the gel slab during a scan. The data from the scan is directly used to amplitude modulate density readings on a display, and the scan is displayed in a horizontal sweep of a cathode ray tube, whereby said cathode ray tube provides intensity displays of bands representing DNA. Different sizes of glass gel sandwiches may be mounted to the same console for different sequencing tasks.

Claims

1. A gel electrophoresis apparatus comprising:

a glass plate-gel-glass plate sandwich having an upper portion and a lower portion;

means for establishing electrical potential across said glass plate-gel-glass plate sandwich;

said means for establishing electrical potential across said glass plate-gel-glass plate sandwich including an upper buffer tank attached to the upper portion of the sandwich;

means for positioning one face of the glass plate-gel-glass plate sandwich against a heater plate; and

said upper buffer tank located on the opposite face of the glass plate-gel-glass plate sandwich with respect to said heater plate in order to accommodate different lengths of glass plate-gel-glass plate sandwiches such that said heater plate can extend at least to the upper portion of the glass plate-gel-glass plate sandwich.

2. A gel electrophoresis glass plate-gel-glass plate sandwich having an upper portion and a lower portion;

said glass plate-gel-glass plate sandwich having means for positioning one face of the glass plate-gel-glass plate sandwich against a heater plate of an gel electrophoresis apparatus;

a buffer tank mounted to the top of the other face of the glass plate-gel-glass plate sandwich;

said upper buffer tank being enclosed on the bottom, two sides and a front side to form a compartment against the glass plate-gel-glass plate; and

the glass plate-gel-glass plate sandwich having a cut away portion below the top edge of said buffer tank, whereby buffer solution may extend over the top edge of the gel.