Preparation of pharmaceutical compositions containing etherlipid-containing multiple lipid liposomes

• US 6,007,839 A
• Filed: 02/02/1998
• Issued: 12/28/1999
• Est. Priority Date: 02/16/1996
• Status: Expired due to Fees

• Current Assignee: Elan Pharmaceuticals Incorporated (Perrigo Company PLC)
• Original Assignee: The Liposome Co., Inc. (Perrigo Company PLC)
• Inventors: Ahmad, Imran, Janoff, Andrew S., Mayhew, Eric, Bhatia, Suresh K.
• Primary Examiner(s): Kishore, Gollamudi S.
• Application Number:
  US09/017,439
• Time in Patent Office: 694 Days
• Field of Search: 424/450, 424/1.21, 424/9.321, 424/9.51, 264/4.1, 264/4.3, 428/402.2
• US Class Current: 424/450
• CPC Class Codes: A61K 9/1272 with substantial amounts of non-phosphatidyl, i.e. non-acylglycerophosphate, surfactants as bilayer-forming substances, e.g. cationic lipids with cholesterol as the only non-phosphatidyl surfactant A61K9/127; lipids as modifying agent A61K47/543
• CPC Class Codes: Y10T 428/2984 Microcapsule with fluid core [includes liposome]

Abstract

Described herein are methods of preparing liposome-containing pharmaceutical compositions, the liposomes containing headgroup-derivatized lipids and etherlipids having the formula: ##STR1## Such compositions are useful in the treatment of a variety of conditions, including various cancers and inflammatory disorders.

Claims

1. A method of preparing a pharmaceutical composition which comprises the steps of:

(a) preparing a liposome having a bilayer comprising a lipid component which comprises an etherlipid and a headgroup-derivatized lipid; and

,

(b) combining the liposome product of step (a) with a pharmaceutically acceptable carrier,

wherein;

the headgroup-derivatized lipid comprises a phosphatidylethanolamine linked at the ethanolamine group to a moiety selected from the group consisting of dicarboxylic acids and polyethylene glycols;

the etherlipid has the formula;

##STR5## R₁ is Y₁ Y₂ ;

Y₂ is CH₃ or CO₂ H;

Y₁ is (CH₂)_n1 (CH═

CH)_n2 (CH₂)_n3 (CH═

CH)_n4 (CH₂)_n5 (CH═

CH)_n6 (CH₂)_n7 (CH═

CH)_n8 (CH₂)_n9 ;

the sum of n1+2n2+n3+2n4+n5+2n6+n7+2n8+n9 is an intefer of from 3 to 23;

n1 is zero or an integer of from 1 to 23;

n3 is zero or an integer of from 1 to 20;

n5 is zero or an integer of from 1 to 17;

n7 is zero or an integer of from 1 to 14;

n9 is zero or an integer of from 1 to 11; and

,

each of n2, n4, n6 and 8 is independently zero or 1;

Z is oxygen or sulfur;

R₂ is an alkyl group or a halogen-substituted alkyl group having the formula (C(X₁)_n10 (X₂)_n11)_n12 CX₃ X₄ X₅ ;

each of X₁, X₂, X₃, X₄ and X₅ is independently H or a halogen atom;

each of n10 and n11 is independently equal to zero, 1 or 2;

n12 is zero or an integer of from 1 to 23;

when n12 is not equal to zero, the sum of n10+n11 is equal to 2; and

,

the headgroup-derivatized lipid comprises from about 5 mole percent to about 20 mole percent of the lipid bilayer and the etherlipid comprises from greater than about 10 mole percent to less than about 30 mole percent of the lipid bilayer.

2. The method of claim 1, wherein step (a) comprises the steps of:

(a) dissolving the lipid component in an organic solvent;

(b) removing the organic solvent from the lipid solution of step (a); and

,

(c) hydrating the product of step (b) with an aqueous phase so as to form liposomes.

3. The method of claim 2, further comprising the step of adding an additional bioactive agent to the organic solvent of step (a) or the aqueous phase of step (c).

4. The method of claim 2, further comprising the step of extruding the liposomes produced in step (c) through a filter so as to produce unilamellar liposomes.

5. The method of claim 4, wherein the filter has a pore size of about 100 nm.

6. The method of claim 4, further comprising the step of loading an additional bioactive agent into the liposome by way of an electrochemical potential across the liposome'"'"'s bilayer.

7. The method of claim 1, wherein the headgroup derivatized lipid comprises dioleoyl phosphatidylethanolamine linked to glutaric acid.

8. The method of claim 1, wherein the etherlipid is:

##STR6##

9. The method of claim 1, wherein the lipid component also comprises cholesterol and dioleoyl phosphatidylcholine.

10. The method of claim 1, wherein the lipid component comprises dioleoyl phosphatidylcholine, cholesterol, dioleoyl phosphatidylethanolamine-glutaric acid and the etherlipid:

and wherein the lipid component comprises about 40 mole percent dioleoyl phosphatidylcholine, about 30 mole percent cholesterol, about 10 mole percent dioleoyl phosphatidylethanolamine-glutaric acid and about 20 mole percent of the etherlipid.