Detection of DNA/RNA by fluorescence polarization
First Claim
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1. A method for detecting amplified target nucleic acid comprising the steps of:
- (a) incubating single stranded, amplified nucleic acid suspected of containing said target nucleic acid with a fluorescein conjugated oligonucleotide probe having complementarity to said target nucleic acid, wherein said fluorescein is linked to said probe through an aminochlorotriazinylaminoalkyl-phosphoryl group;
(b) incubating for a time sufficient for hybridization of said oligonucleotide probe with said target nucleic acid to occur; and
(c) detecting said target nucleic acid by measuring the degree of fluorescence polarization.
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Abstract
A homogeneous method for detecting amplified RNA or DNA target sequences utilizes signal-labelled DNA or RNA probes which show detectably increased fluorescence polarization when hybridized to target sequences. A convenient one-step analytical procedure requiring no nucleic acid extraction or signal separation step is thereby provided.
44 Citations
1 Claim
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1. A method for detecting amplified target nucleic acid comprising the steps of:
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(a) incubating single stranded, amplified nucleic acid suspected of containing said target nucleic acid with a fluorescein conjugated oligonucleotide probe having complementarity to said target nucleic acid, wherein said fluorescein is linked to said probe through an aminochlorotriazinylaminoalkyl-phosphoryl group; (b) incubating for a time sufficient for hybridization of said oligonucleotide probe with said target nucleic acid to occur; and (c) detecting said target nucleic acid by measuring the degree of fluorescence polarization.
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Specification
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Current AssigneeAstrazeneca UK Limited (Astrazeneca PLC)
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Original AssigneeZeneca Incorporated (Astrazeneca PLC)
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InventorsWang, Chao-huei Jeffrey, Ammons, Harryl, Jolley, Michael E.
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Primary Examiner(s)PRIEBE, SCOTT DAVID
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Application NumberUS08/247,629Time in Patent Office2,045 DaysField of Search435/6, 435/91.2, 536/24.3US Class Current435/6.11CPC Class CodesC12Q 1/6816 characterised by the detect...C12Q 2563/107 fluorescence
