Non-invasive diagnostic method for interstitial cystitis and bladder cancer
First Claim
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1. A method of diagnosing or monitoring bladder cancer in a mammal comprising determining concentration of a urine-soluble protein selected from the group consisting of neurotrophin-3, glial cell line-derived neurotrophic factor, tryptase, and combinations thereof in urine from the mammal and from a control population of mammals with no history of bladder cancer;
- and then comparing the concentrations from the mammal with corresponding concentrations from the control population, wherein elevated levels of neurotrophin-3, glial cell line-derived neurotrophic factor, or tryptase in the mammal as compared to the control population is indicative of bladder cancer in the mammal.
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Abstract
Disclosed is a method of diagnosing or monitoring interstitial cystitis or bladder cancer in a mammal. The method comprises analyzing urine of the mammal for the concentration of a urine-soluble protein selected from the group consisting of neurotrophin-3, nerve growth factor, glial cell line-derived neurotrophic factor, tryptase, and combinations thereof.
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Citations
25 Claims
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1. A method of diagnosing or monitoring bladder cancer in a mammal comprising determining concentration of a urine-soluble protein selected from the group consisting of neurotrophin-3, glial cell line-derived neurotrophic factor, tryptase, and combinations thereof in urine from the mammal and from a control population of mammals with no history of bladder cancer;
- and then comparing the concentrations from the mammal with corresponding concentrations from the control population, wherein elevated levels of neurotrophin-3, glial cell line-derived neurotrophic factor, or tryptase in the mammal as compared to the control population is indicative of bladder cancer in the mammal.
- View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A non-invasive method of diagnosing or monitoring bladder cancer in a human subject comprising:
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(a) securing a urine sample from the human subject and from a control population of humans with no history of bladder cancer; and
then(b) determining the presence and concentration in the urine sample from the human subject and from the control population of humans of a urine-soluble protein selected from the group consisting of neurotrophin-3, glial cell line-derived neurotrophic factor, tryptase, and combinations thereof; and
then comparing the concentrations from the human subject with corresponding concentrations from the control population, wherein elevated levels of neurotrophin-3, glial cell line-derived neurotrophic factor, or tryptase in the human subject as compared to the control population is indicative of bladder cancer in the human subject. - View Dependent Claims (9, 10, 11, 12, 13)
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14. A method of diagnosing or monitoring bladder cancer in a human subject comprising:
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(a) securing a urine sample from the human subject and from a control population of humans with no history of bladder cancer; and
then(b) acidifying the urine samples from the human subject and from the control population to a pH of about 3.0 or less and then neutralizing the urine samples; and
then(c) determining concentration in the urine samples from the human subject and from the control population of a urine-soluble protein selected from the group consisting of neurotrophin-3, glial cell line-derived neurotrophic factor, tryptase, and combinations thereof using a corresponding double antibody-sandwich enzyme-linked immunosorbent assay specific for the urine-soluble protein being analyzed; and
then comparing the concentrations from the human subject with corresponding concentrations from the control population, wherein elevated levels of neurotrophin-3, glial cell line-derived neurotrophic factor, or tryptase in the human subject as compared to the control population is indicative of bladder cancer in the human subject.
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15. A method of diagnosing or monitoring interstitial cystitis in a mammal comprising determining concentration of a urine-soluble protein selected from the group consisting of neurotrophin-3, glial cell line-derived neurotrophic factor, and combinations thereof in urine from the mammal and from a control population of mammals with no history of interstitial cystitis;
- and then comparing the concentrations from the mammal with corresponding concentrations from the control population, wherein elevated levels of neurotrophin-3 or glial cell line-derived neurotrophic factor in the mammal as compared to the control population is indicative of interstitial cystitis in the mammal.
- View Dependent Claims (16, 17, 18, 19)
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20. A non-invasive method of diagnosing or monitoring interstitial cystitis in a human subject comprising:
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(a) securing a urine sample from the human subject and from a control population of humans with no history of interstitial cystitis; and
then(b) determining the presence and concentration in the urine sample from the human subject and from the control population of humans of a urine-soluble protein selected from the group consisting of neurotrophin-3, glial cell line-derived neurotrophic factor, and combinations thereof; and
then comparing the concentrations from the human subject with corresponding concentrations from the control population, wherein elevated levels of neurotrophin-3 or glial cell line-derived neurotrophic factor in the human subject as compared to the control population is indicative of interstitial cystitis or bladder cancer in the human subject. - View Dependent Claims (21, 22, 23, 24)
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25. A method of diagnosing or monitoring interstitial cystitis in a human subject comprising:
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(a) securing a urine sample from the human subject and from a control population of humans with no history of interstitial cystitis; and
then(b) acidifying the urine samples from the human subject and from the control population to a pH of about 3.0 or less and then neutralizing the urine samples; and
then(c) determining concentration in the urine samples from the human subject and from the control population of a urine-soluble protein selected from the group consisting of neurotrophin-3, glial cell line-derived neurotrophic factor, and combinations thereof using a corresponding double antibody-sandwich enzyme-linked immunosorbent assay specific for the urine-soluble protein being analyzed; and
then comparing the concentrations from the human subject with corresponding concentrations from the control population, wherein elevated levels of neurotrophin-3 or glial cell line-derived neurotrophic factor in the human subject as compared to the control population is indicative of interstitial cystitis in the human subject.
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Specification