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Fluorescent labeling complexes with large stokes shift formed by coupling together cyanine and other fluorochromes capable of resonance energy transfer

  • US 6,008,373 A
  • Filed: 06/07/1995
  • Issued: 12/28/1999
  • Est. Priority Date: 06/07/1995
  • Status: Expired due to Term
First Claim
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1. A low molecular weight fluorescent labeling complex for labeling a target material consisting of:

  • a first fluorochrome having first absorption and emission spectra;

    a second fluorochrome having second absorption and emission spectra, the wavelength of the emission maximum of said second fluorochrome being longer than the wavelength of the emission maximum of said first fluorochrome, and a portion of the absorption spectrum of said second fluorochrome overlapping a portion of the emission spectrum of said first fluorochrome for transfer of energy absorbed by said first fluorochrome when said complex is excited by light to said second fluorochrome;

    a linker for covalently attaching said first and second fluorochromes capable of transfer of resonance energy between said first and second fluorochromes;

    at least one of said first or second fluorochromes is a cyanine dye and the combined molecular weight of said first and second fluorochromes and said linker is less than about 20,000 Daltons; and

    at least one group capable of forming a covalent bond with said target material.

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