Spectrometer system for diagnosis of skin disease

• US 6,008,889 A
• Filed: 04/16/1997
• Issued: 12/28/1999
• Est. Priority Date: 04/16/1997
• Status: Expired due to Fees

• Current Assignee: Derma Art Technologies, Inc.
• Original Assignee: Derma Art Technologies, Inc.
• Inventors: Zeng, Haishan, Lui, Harvey, McLean, David I., MacAulay, Calum, Palcic, Branko
• Primary Examiner(s): Kim, Robert H.
• Application Number:
  US08/843,605
• Time in Patent Office: 986 Days
• Field of Search: 356/73, 356/318, 356/326, 356/445-448, 128/633, 128/664, 128/665, 385/901, 385/116, 600/473-476
• US Class Current: 356/73
• CPC Class Codes: A61B 5/0059 using light, e.g. diagnosis by transillumination, diascopy, fluorescence A61B5/0093, A61B5/1455, A61B5/02416 and A61B5/0261 take precedence
• CPC Class Codes: A61B 5/0071 by measuring fluorescence emission
• CPC Class Codes: A61B 5/444 Evaluating skin marks, e.g. mole, nevi, tumour, scar
• CPC Class Codes: G01N 2021/475 Bifurcated bundle
• CPC Class Codes: G01N 2021/6417 Spectrofluorimetric devices
• CPC Class Codes: G01N 2021/656 Raman microprobe
• CPC Class Codes: G01N 21/474 Details of optical heads therefor, e.g. using optical fibres
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters
• CPC Class Codes: G01N 21/6486 Measuring fluorescence of biological material, e.g. DNA, RNA, cells G01N21/6428 takes precedence
• CPC Class Codes: G01N 21/65 Raman scattering
• CPC Class Codes: G01N 2201/0627 Use of several LED's for spectral resolution

Abstract

Apparatus for diagnosis of a skin disease site using spectral analysis includes a light source for generating light to illuminate the disease site and a probe unit optically connected to the light source for exposing the disease site to light to generate fluorescence and reflectance light. The probe unit also collects the generated fluorescence and reflectance light and transmits this light to a spectrometer to be analysed. The spectrometer generates and displays spectral measurements of the fluorescence light and the reflectance light which in together assist the user in diagnosing the disease site. The apparatus makes use of a conventional personal computer using a plug-in spectrometer card to provide a compact and low cost system. The system performs combined fluorescence and reflectance spectral analysis in a quick and efficient manner to provide a powerful tool for dermatologic diagnosis.

Claims

1. Apparatus able to diagnose a disease selected from the group consisting of actinic keratosis, seborrheic keratosis, basal cell carcinoma and compound nevus at a skin disease site using spectral analysis comprising:

a light source for generating light to illuminate the disease site;

a probe means optically connected to the light source to expose the disease site to light to generate fluorescence and reflectance light and collect said generated fluorescence and reflectance light;

spectral analysis means optically connected to the probe means for generating spectral measurements of the fluorescence light and the reflectance light to assist the user in diagnosing the disease site;

a plurality of comparison spectra corresponding to, and able to distinguish between, each of actinic keratosis, seborrheic keratosis, basal cell carcinoma and compound nevus wherein the spectra indicate that if the reflectance curve slope is similar to normal and the fluorescence intensity is higher than normal, then the disease is actinic keratosis;

if the reflectance curve slope is similar to normal and the fluorescence intensity is lower than normal, then the disease is basal cell carcinoma;

if the reflectance curve slope is larger than normal and the fluorescence intensity is higher than normal, then the disease is seborrheic keratosis; and

, if the reflectance curve slope is larger than normal and the fluorescence intensity is lower than normal, then the disease is compound nevus; and

a display indicating the presence or absence at the skin disease site of actinic keratosis, seborrheic keratosis, basal cell carcinoma or compound nevus.

2. Apparatus as claimed in claim 1 in which the light source comprises an excitation light source and a white light source.

3. Apparatus as claimed in claim 2 in which the excitation light source is a Helium-Cadmium laser and the white light source is a quartz tungsten halogen lamp.

4. Apparatus as claimed in claim 2 including a shutter system to switch between the excitation light source and the white light source such that the disease site is illuminated sequentially by one of the light sources at a time.

5. Apparatus as claimed in claim 4 including computer control means to control the shutter system by switching between light sources and to control the spectral analysis means in order to acquire spectral measurements of the fluorescence and reflectance light.

6. Apparatus as claimed in claim 5 in which the computer control means comprises a personal computer and the spectral analysis means comprises a computer interface card installable in an interface slot of the computer to function as a spectrometer.

7. Apparatus as claimed in claim 6 in which the spectrometer computer interface card includes a miniature monochromator, a CCD linear array detector, means for optically connecting to the probe means, and data acquisition and controlling circuitry.

8. Apparatus as claimed in claim 2 including a light filter selected to pass fluorescence light and block reflected excitation light.

9. Apparatus as claimed in claim 2 in which the light source is optically connected to the probe means and the probe means is optically connected to the spectral analysis means by optical fibre bundles.

10. Apparatus as claimed in claim 9 including an optical coupler for connecting the white light source and the excitation light source to deliver light via the same optical fibre bundle.

11. Apparatus as claimed in claim 10 in which the optical coupler comprises:

an enclosure housing, an angled reflecting surface having an aperture;

a laser light port in the enclosure aligned with the aperture to permit passage of laser light directly through the angled reflecting surface, the laser light port being optically connectable to a laser light source;

a light exit port from the enclosure aligned with the laser light port and the aperture through the angled reflecting surface, the light exit port being optically connectable to the probe means; and

a white light port in the enclosure positioned with respect to the angled reflecting surface such that white light is reflected through an angle to be incident upon the light exit port, the white light opening being optically connectable to the white light source.

12. Apparatus as claimed in claim 9 in which the probe means comprises an elongate member having an open end defining a chamber with opaque walls that is positionable over the disease site to exclude external light from the chamber, the chamber being connected to the optical fibre bundles for receiving light from the light sources and transmitting fluorescence light and reflectance light to the spectral analysis means.

13. Apparatus as claimed in claim 12 in which the elongate member comprises:

a generally cylindrical member housing the optical fibre bundles that extend to a distal end of the cylindrical member;

a sleeve member that telescopes over the generally cylindrical housing, the overlap of the sleeve member with the distal end of the cylindrical member defining the chamber whereby slidable movement of the sleeve member with respect to the cylindrical member adjusts the length of the chamber to vary the size of the illumination spot at the disease site.

14. Apparatus as claimed in claim 13 in which the sleeve member is formed with a window for viewing inside the chamber to ensure proper positioning of the chamber over the disease site and a slidable cover to seal the window when the apparatus is in use.

15. Apparatus as claimed in claim 13 in which the free end of the sleeve member is cut at substantially 45 degrees to the longitudinal axis of the sleeve.

16. Apparatus as claimed in claim 1 wherein the apparatus further comprises a comparison spectrum able to distinguish spider angioma from each of actinic keratosis, seborrheic keratosis, basal cell carcinoma and compound nevus, wherein the spectrum indicates that if the fluorescence intensity is lower than normal and the reflectance ratio spectrum comprises a saw-tooth shape with a minimum at about 600 nm. then the disease is slider angioma, and the display indicates the presence or absence at the skin disease site of actinic keratosis, seborrheic keratosis, basal cell carcinoma, compound nevus or spider angioma.

17. Apparatus as claimed in claim 1 wherein the apparatus further comprises a comparison spectrum able to distinguish psoriasis from each of actinic keratosis, seborrheic keratosis, basal cell carcinoma and compound nevus, wherein the spectrum indicates that if the fluorescence spectral curve comprises an extra peak at about 635 nm. Then the disease is psoriasis, and the display indicates the presence or absence at the skin disease site of actinic keratosis, seborrheic keratosis, basal cell carcinoma, compound nevus or psoriasis.

18. Apparatus as claimed in claim 1 wherein the apparatus further comprises a comparison spectrum is able to distinguish squamous cell carcinoma from each of actinic keratosis, seborrheic keratosis, basal cell carcinoma and compound nevus, wherein the spectrum indicates that if the fluorescence intensity is lower than normal in some areas within the lesion and higher than normal in other areas within the lesion, then the disease is squamous cell carcinoma, and the display indicates the presence or absence at the skin disease site of actinic keratosis, seborrheic keratosis, basal cell carcinoma, compound nevus or squamous cell carcinoma.

19. Apparatus as claimed in claim 1 wherein the comparison spectra are stored in a computer.

20. An apparatus as claimed in claim 1 wherein the fluorescence is autofluorescence.

21. A method for diagnosis of a disease selected from the group consisting of actinic keratosis, seborrheic keratosis, basal cell carcinoma, compound nevus and spider angioma at a skin disease site using spectral analysis comprising the steps of:

illuminating the disease site with a light source to generate fluorescence and reflectance light at the disease site;

collecting the generated fluorescence and reflectance light;

conducting a spectral analysis of the collected light using a spectrometer;

determining spectral measurements of the fluorescence light and the reflectance light;

examining the spectral measurements together to make a diagnosis of the disease site, wherein the step of examining comprises comparing the spectral measurements with known spectral measurements and wherein if the reflectance curve slope is similar to normal and the fluorescence intensity is higher than normal, then determining that the disease is actinic keratosis;

if the reflectance curve slope is similar to normal and the fluorescence intensity is lower than normal, then determining that the disease is basal cell carcinoma;

if the reflectance curve slope is larger than normal and the fluorescence intensity is higher than normal, then determining that the disease is seborrheic keratosis; and

, if the reflectance curve slope is larger than normal and the fluorescence intensity is lower than normal, then determining that the disease is compound nevus; and

,

displaying the diagnosis.

22. A method as claimed in claim 21 in which the steps of illuminating the disease site and collecting generated light involves sequentially illuminating the site with white light and collecting reflectance light and then illuminating the site with excitation light and collecting fluorescence light or vice versa.

23. A method as claimed in claim 22 including filtering the collected fluorescence light to filter out the excitation light.

24. A method as claimed in claim 21 in which a spectrometer card mounted in a personal computer is used to conduct a spectral analysis of the generated fluorescence and reflected light and display spectral measurements.

25. A method as claimed in claim 21 in which the step of collecting the generated fluorescence and reflectance light involves collecting light from the disease site and the surrounding normal skin.

26. A method as claimed in claim 25 in which the step of examining the spectral measurements involves comparing the disease site spectrum to the spectrum of the surrounding normal skin.

27. A method as claimed in claim 26 including the step of generating a ratio spectrum by dividing the disease site spectrum by the spectrum of the adjacent normal skin.

28. A method as claimed in claim 21 wherein, in the step of examining, if the fluorescence intensity is lower than normal and the reflectance ratio spectrum comprises a saw-tooth shape with a minimum at about 600 nm, then determining that the disease is spider angioma.

29. A method as claimed in claim 21 wherein, in the step of examining, if the fluorescence spectral curve comprises an extra peak at about 635 nm, then determining that the disease is psoriasis.

30. A method as claimed in claim 21 wherein, in the step of examining, if the fluorescence intensity is lower than normal in some areas within the lesion and higher than normal in other areas within the lesion, then determining that the disease is squamous cell carcinoma.

31. A method as claimed in claim 21 wherein the fluorescence is autofluorescence.

32. A combination able to diagnose a disease selected from the group consisting of actinic keratosis, seborrheic keratosis, basal cell carcinoma and compound nevus at a skin disease site using spectral analysis comprising:

a light source for generating light to illuminate the disease site;

an optical probe optically connected to the light source to expose the disease site to light to generate fluorescence and reflectance light;

a spectral analyzer optically connected to the optical probe for generating and displaying spectral measurements of the fluorescence light and the reflectance light to assist the user in diagnosing the disease site;

a plurality of comparison spectra corresponding to, and able to distinguish between, each of actinic keratosis, seborrheic keratosis, basal cell carcinoma and compound nevus, wherein the spectra indicate that if the reflectance curve slope is similar to normal and the fluorescence intensity is higher than normal, then the disease is actinic keratosis;

if the reflectance curve slope is similar to normal and the fluorescence intensity is lower than normal, then the disease is basal cell carcinoma;

if the reflectance curve slope is larger than normal and the fluorescence intensity is higher than normal, then the disease is seborrheic keratosis; and

, if the reflectance curve slope is larger than normal and the fluorescence intensity is lower than normal, then the disease is compound nevus; and

a display indicating the presence or absence at the skin disease site, of actinic keratosis, seborrheic keratosis, basal cell carcinoma or compound nevus at the skin disease site.

33. A combination as claimed in claim 32 wherein the combination further comprises a comparison spectrum able to distinguish psoriasis from each of actinic keratosis, seborrheic keratosis, basal cell carcinoma and compound nevus, wherein the spectrum indicates that if the fluorescence spectral curve comprises an extra peak at about 635 nm, then the disease is psoriasis, and the display indicates the presence or absence at the skin disease site of actinic keratosis, basal cell carcinoma, compound nevus or psoriasis.

34. A combination as claimed in claim 33 wherein the comparison spectra are stored in a computer.

35. A combination as claimed in claim 32 wherein the combination further comprises a comparison spectrum able to distinguish squamous cell carcinoma from each of actinic keratosis, seborrheic keratosis, basal cell carcinoma and compound nevus wherein the spectrum indicates that if the fluorescence intensity is lower than normal in some areas within the lesion and higher than normal in other areas within the lesion, then the disease is squamous cell carcinoma, and the display indicates the presence or absence at the skin disease site of actinic keratosis, seborrheic keratosis, basal cell carcinoma, compound nevus or squamous cell carcinoma.

36. A combination as claimed in claim 35 wherein the comparison spectra are stored in a computer.

37. A combination as claimed in claim 32 wherein the comparison spectra are stored in a computer.

38. A combination as claimed in claim 32 wherein the fluorescence is autofluorescence.

39. A combination as claimed in 32 wherein the apparatus further comprises a comparison spectrum able to distinguish spider angioma from each of actinic keratosis, seborrheic keratosis, basal cell carcinoma and compound nevus, wherein the spectrum indicates that if the fluorescence intensity is lower than normal and the reflectance ratio spectrum comprises a saw-tooth shape with a minimum at about 600 nm, then the disease is spider angioma, and the display indicates the presence or absence at the skin disease site of actinic keratosis, seborrheic keratosis, basal cell carcinoma, compound nevus or spider angioma.