Enhancing hematopoietic progenitor cell engraftment using mesenchymal stem cells

• US 6,010,696 A
• Filed: 03/25/1998
• Issued: 01/04/2000
• Est. Priority Date: 11/16/1990
• Status: Expired due to Fees

• Current Assignee: Mesoblast International Srl
• Original Assignee: Osiris Therapeutics Incorporated (Smith & Nephew PLC)
• Inventors: Lazarus, Hillard M., Haynesworth, Stephen E., Caplan, Arnold I., Gerson, Stanton L.
• Primary Examiner(s): Lankford, Jr., Leon B.
• Assistant Examiner(s): Tate, Christopher R.
• Application Number:
  US09/047,950
• Time in Patent Office: 650 Days
• Field of Search: 424/93.7, 424/422, 424/423, 424/548, 424/549, 424/577, 435/325, 435/366, 435/372, 435/373, 435/372.1-372.3, 435/377
• US Class Current: 424/93.7
• CPC Class Codes: A61F 2/28 Bones joints A61F2/30
• CPC Class Codes: A61F 2/30756 Cartilage endoprostheses A61F2/3603 takes precedence
• CPC Class Codes: A61K 2035/124 the cells being hematopoietic, bone marrow derived or blood cells
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 35/28 Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
• CPC Class Codes: A61L 2430/10 for reconstruction of tendons or ligaments
• CPC Class Codes: A61L 27/3804 characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
• CPC Class Codes: A61L 27/3834 Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells
• CPC Class Codes: A61L 27/3886 comprising two or more cell types
• CPC Class Codes: A61L 31/005 Ingredients of undetermined constitution or reaction products thereof
• CPC Class Codes: C07K 16/28 against receptors, cell surface antigens or cell surface determinants
• CPC Class Codes: C12N 2501/23 Interleukins [IL]
• CPC Class Codes: C12N 2501/39 Steroid hormones
• CPC Class Codes: C12N 2533/18 Calcium salts, e.g. apatite, Mineral components from bones, teeth, shells
• CPC Class Codes: C12N 5/0663 Bone marrow mesenchymal stem cells (BM-MSC)

Abstract

Method and preparations for enhancing hematopoietic progenitor cell engraftment in an individual by administering (i) a culturally expanded mesenchymal stem cell preparation and (ii) hematopoietic progenitor cells. The mesenchymal stem cells are administered in an amount effective to promote engraftment of the hematopoietic progenitor cells.

Claims

1. A method for enhancing engraftment of hematopoietic progenitor cells in an individual in need thereof which comprises administering to said individual (i) isolated, homogeneous mesenchymal stem cells and (ii) hematopoietic progenitor cells, wherein said mesenchymal stem cells are administered in an amount effective to promote engraftment of said hematopoietic progenitor cells in said individual.

2. The method of claim 1 wherein the mesenchymal stem cells are administered by intravenous injection or by injection directly to the site of intended activity.

3. The method of claim 1 wherein at least one of (i) the mesenchymal stem cells and (ii) the hematopoietic progenitor cells is administered intravenously.

4. The method of claim 1 wherein at least one of (i) the mesenchymal stem cells and (ii) the hematopoietic progenitor cells is administered by injection directly to the site of intended activity.

5. The method of claim 1 wherein the mesenchymal stem cells are autologous.

6. The method of claim 1 wherein the mesenchymal stem cell preparation is administered concurrently with the hematopoietic progenitor cells.

7. The method of claim 6 wherein the mesenchymal stem cells are introduced into said individual in a cell suspension also containing hematopoietic progenitor cells.

8. The method of claim 1 wherein the mesenchymal stem cell preparation is administered to the individual prior to administration of the hematopoietic progenitor cells.