Recombinant binding proteins and peptides

• US 6,010,884 A
• Filed: 05/29/1996
• Issued: 01/04/2000
• Est. Priority Date: 12/04/1992
• Status: Expired due to Fees

• Current Assignee: Medical Research Council (Government of United Kingdom)
• Original Assignee: Medical Research Council (Government of United Kingdom)
• Inventors: Fisch, Igor, Nissim, Ahuva, Griffiths, Andrew David, Winter, Gregory Paul, Holliger, Kaspar Philipp
• Primary Examiner(s): ULM, JOHN D
• Application Number:
  US08/654,623
• Time in Patent Office: 1,315 Days
• Field of Search: 435/69.1, 435/69.7, 435/69.8, 435/172.3, 435/252.3, 435/254.11, 435/320.1, 435/325, 536/23.4, 536/23.53, 536/24.1
• US Class Current: 435/69.7
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 16/00 Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies antibodies with enzymatic activity, e.g. abzymes C12N9/0002
• CPC Class Codes: C07K 16/005 constructed by phage libraries
• CPC Class Codes: C07K 16/40 against enzymes
• CPC Class Codes: C07K 16/44 against material not provided for elsewhere , e.g. haptens, metals, DNA, RNA, amino acids
• CPC Class Codes: C07K 16/468 Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
• CPC Class Codes: C07K 2317/34 Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
• CPC Class Codes: C07K 2317/622 Single chain antibody (scFv)
• CPC Class Codes: C07K 2317/626 Diabody or triabody
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: C07K 2319/02 containing a signal sequence
• CPC Class Codes: C07K 2319/41 containing a Myc-tag
• CPC Class Codes: C07K 2319/735 containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)
• CPC Class Codes: C12N 15/1037 Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
• CPC Class Codes: C12N 15/113 Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing when used in plants C12N15/8218
• CPC Class Codes: C12N 2310/111 spanning the whole gene, or a large part of it
• CPC Class Codes: C12N 2310/1241 Tetrahymena
• CPC Class Codes: C12N 2310/127 DNAzymes
• CPC Class Codes: C40B 40/02 Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors

Abstract

DNA constructs comprise a first exon sequence of nucleotides encoding a first peptide or polypeptide, a second exon sequence of nucleotides encoding a second peptide or polypeptide and a third sequence of nucleotides between the first and second sequences encoding a heterologous intron, for example that of Tetrahymena thermophila nuclear pre-rRNA, between RNA splice sites and a site-specific recombination sequence, such as loxP, within the intron, the exons together encoding a product peptide or polypeptide. Such constructs are of use in methods of production of peptides or polypeptides, transcription leading to splicing out of the intron enabling translation of a single chain product peptide or polypeptide. Isolated nucleic acid constructs consisting essentially of a sequence of nucleotides encoding a self-splicing intron with a site-specific recombination sequence within the intron, for use in creation of constructs for expression of peptides or polypeptides, are also provided.

Claims

1. A DNA construct comprising a first exon sequence of nucleotides encoding a first peptide or polypeptide, a second exon sequence of nucleotides encoding a second peptide or polypeptide and a third sequence of nucleotides between the first and second sequences encoding a heterologous intron between RNA splice sites and a site-specific recombinase recognition sequence within the intron, the exons together encoding a product peptide or polypeptide.

2. A DNA construct according to claim 1 wherein the product peptide or polypeptide comprises a member of a specific binding pair (sbp).

3. A DNA construct according to claim 2 wherein the sbp member comprises a binding domain able to bind complementary sbp member.

4. A DNA construct according to claim 3 wherein the binding domain is an immunoglobulin antigen-binding site.

5. A DNA construct according to claim 4 wherein the product peptide or polypeptide is a scFv antibody fragment which comprises a VH domain linked to a VL domain via a peptide linker which allows the VH and VL domains to associate to form the antigen-binding site.

6. A DNA construct according to claim 5 wherein the intron is selected from a group consisting of a self-splicing group I intron and a self-splicing group II intron.

7. A DNA construct according to claim 5 wherein the product peptide or polypeptide comprises a surface component of an organism.

8. A DNA construct according to claim 5 which is a vector further comprising nucleic acid for expression of the product peptide or polypeptide.

9. A method of producing a product peptide or polypeptide which comprises a combination of a first peptide or polypeptide component and a second peptide or polypeptide component, the method comprising:

providing a DNA construct according to claim 8;

transcribing DNA of the construct into RNA;

causing or allowing splicing of nucleotides of the third sequence to produce an RNA molecule encoding the product peptide or polypeptide;

translating the RNA molecule into the product peptide or polypeptide.

10. A method according to claim 9 wherein a plurality of DNA constructs is provided for transcription, splicing and translation.

11. A method according to claim 10 wherein, following said translation, product peptide or polypeptide of interest is selected or isolated from other peptides or polypeptides present.

12. A plurality of DNA constructs according to claim 5 collectively encoding a repertoire of product peptide or polypeptides wherein each product peptide or polypeptide in the repertoire has a different amino acid sequence.

13. A plurality of host cells comprising a plurality of DNA constructs according to claim 12.

14. A DNA construct according to claim 5 wherein transcription of said construct leads to mRNA having nucleotides corresponding to nucleotides of said RNA splice sites which encode and are translatable into amino acids of the peptide linker of said scFv antibody fragment.

15. A DNA construct according to claim 1 wherein the product peptide or polypeptide is selected from the group consisting of a T cell receptor V.sub.α

and a T cell receptor V.sub.β

domain, a T cell receptor/antibody fusion, a T cell receptor/antibody fragment fusion, a receptor binding peptide, an enzyme, a multiple domain protein, an amino acid sequence variant of said peptide or polypeptide and a derivative of said peptide or polypeptide.

16. A DNA construct according to claim 1 wherein the product peptide comprises a binding domain able to bind a complementary sbp member.

17. A DNA construct according to claim 16 wherein one of said first and second peptides or polypeptides comprises an antibody fragment.

18. A DNA construct according to claim 17 wherein the antibody fragment is selected from the group consisting of VH, VL, CH, CL, VH--CH and VL--CL.

19. DNA construct according to claim 16 wherein the first and/or second peptides or polypeptides comprise an amino acid sequence encoded by a synthetic nucleotide sequence.

20. A DNA construct according to claim 16 wherein the intron is selected from a group consisting of a self-splicing group I intron and a self-splicing group II intron.

21. A DNA construct according to claim 16 wherein the product peptide or polypeptide comprises a surface component of an organism.

22. A DNA construct according to claim 16 which is a vector further comprising nucleic acid for expression of the product peptide or polypeptide.

23. A method of producing a product peptide or polypeptide which comprises a combination of a first peptide or polypeptide component and a second peptide or polypeptide component, the method comprising:

providing a DNA construct according to claim 22;

transcribing DNA of the construct into RNA;

causing or allowing splicing of nucleotides of the third sequence to produce an RNA molecule encoding the product peptide or polypeptide;

translating the RNA molecule into the product peptide or polypeptide.

24. A method according to claim 23 wherein a plurality of DNA constructs is provided for transcription, splicing and translation.

25. A method according to claim 24 wherein, following said translation, product peptide or polypeptide of interest is selected or isolated from other peptides or polypeptides present.

26. A plurality of DNA constructs according to claim 16 collectively encoding a repertoire of product peptide or polypeptides wherein each product peptide or polypeptide in the repertoire has a different amino acid sequence.

27. A plurality of host cells comprising a plurality of DNA constructs according to claim 26.

28. A DNA construct according to claim 1 wherein the intron is selected from a group consisting of a self-splicing group I intron and a self-splicing group II intron.

29. A DNA construct according to claim 28 wherein the self-splicing intron is obtainable from Tetrahymena thermophila nuclear pre-rRNA.

30. A DNA construct according to claim 1 wherein the site-specific recombinase recognition sequence is the loxP sequence obtainable from coliphage P1, or a mutant or derivative of said loxP sequence.

31. A DNA construct according to claim 1 wherein the product peptide or polypeptide comprises a surface component of an organism.

32. A DNA construct according to claim 31 wherein the organism is a bacteriophage.

33. A DNA construct according to claim 32 wherein the bacteriophage is selected from a group consisting of fd and M13.

34. A DNA construct according to claim 33 wherein the surface component is the gene III product.

35. A DNA construct according to claim 1 which is a vector further comprising nucleic acid for expression of the product peptide or polypeptide.

36. A DNA construct according to claim 35 further comprising nucleic acid for secretion of the product peptide or polypeptide.

37. A DNA construct according to claim 36 wherein said vector is selected from a group consisting of a plasmid, a phage, and a phagemid vector.

38. A method of producing a product peptide or polypeptide which comprises a combination of a first peptide or polypeptide component and a second peptide or polypeptide component, the method comprising:

providing a DNA construct according to claim 35;

transcribing DNA of the construct into RNA;

causing or allowing splicing of nucleotides of the third sequence to produce an RNA molecule encoding the product peptide or polypeptide;

translating the RNA molecule into the product peptide or polypeptide.

39. A method according to claim 38 wherein transcription, splicing and translation take place in vitro.

40. A method according to claim 39 wherein transcription, splicing and translation take place in vivo.

41. A method according to claim 40 wherein transcription, splicing and translation take place in E. coli cells.

42. A method according to claim 38 wherein a plurality of DNA constructs is provided for transcription, splicing and translation.

43. A method according to claim 42 wherein, following said translation, product peptide or polypeptide of interest is selected or isolated from other peptides or polypeptides present.

44. A method according to claim 38 wherein, following said translation, product peptide or polypeptide of interest is selected or isolated from other peptides or polypeptides present.

45. A host cell comprising a DNA construct according to claim 1.

46. A plurality of DNA constructs according to claim 1 collectively encoding a repertoire of product peptide or polypeptides wherein each product peptide or polypeptide in the repertoire has a different amino acid sequence.

47. A population of host cells comprising a plurality of DNA constructs according to claim 46.

48. An isolated nucleic acid construct consisting essentially of a self-splicing intron with a site-specific recombinase recognition sequence within the intron.

49. A nucleic acid construct according to claim 48 wherein the self-splicing intron is obtainable from Tetrahymena thermophila nuclear pre-rRNA.

50. A nucleic acid construct according to claim 48 wherein the site-specific recombinase recognition sequence is the loxP sequence obtainable from coliphage P1 or a mutant or derivative of said loxP sequence.