Micropump

• US 6,012,902 A
• Filed: 09/25/1997
• Issued: 01/11/2000
• Est. Priority Date: 09/25/1997
• Status: Expired due to Term

• Current Assignee: Caliper Technologies Corp (Revvity, Inc.)
• Original Assignee: Caliper Technologies Corp (Revvity, Inc.)
• Inventors: Parce, John Wallace
• Primary Examiner(s): Freay, Charles G.
• Assistant Examiner(s): TYLER, CHERYL JACKSON
• Application Number:
  US08/937,958
• Time in Patent Office: 838 Days
• Field of Search: 417/48, 417/50
• US Class Current: 417/48
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2400/0418 electro-osmotic flow [EOF]
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: F04B 19/006 Micropumps F04B43/043 and F04B43/095 take precedence
• CPC Class Codes: H02N 11/006 Motors

Abstract

The present invention generally provides a micropump that utilizes electroosmotic pumping of fluid in one channel or region to generate a pressure based flow of material in a connected channel, where the connected channel has substantially no electroosmotic flow generated. Such pumps have a variety of applications, and are particularly useful in those situations where the application for which the pump is to be used prohibits the application of electric fields to the channel in which fluid flow is desired, or where pressure based flow is particularly desirable.

Claims

1. A microfluidic device, comprising:

a body structure comprising a substrate layer having at least one microscale channel fabricated into a surface thereof; and

an integrated micropump in fluid communication with the at least one microscale channel, the micropump comprising;

a first fluid containing microscale channel portion having first and second ends;

a second fluid containing microscale channel portion having first and second ends, the second channel portion having a first effective surface charge associated with walls of the second channel portion, the first end of the second channel portion in fluid communication with the first end of the first channel portion at a first channel junction; and

a means for applying a voltage gradient between the first and second ends of the second fluid containing channel portion to electroosmotically move fluid through the second channel portion while applying substantially no voltage gradient between the first and second ends of the first fluid containing channel portion.

2. The microfluidic device of claim 1, wherein the second channel portion has a smaller cross sectional area than the first channel portion.

29. The microfluidic device of claim 1, 3, 7, 11, 14, 15, 16, 17 or 20, further comprising a cover layer mated with the surface of the substrate to seal the channel or channel portions.

3. A microfluidic device comprising:

a body structure comprising a substrate layer having at least a first microscale channel fabricated into a surface thereof; and

an integrated micropump in fluid communication with at least one microscale channel, the micropump comprising;

a first fluid containing microscale channel portion having first and second ends;

a second fluid containing microscale channel portion having first and second ends, the second channel portion having a first effective surface charge associated with walls of the second channel portion, the first end of the second channel portion in fluid communication with the first end of the first channel portion at a first channel junction; and

a means for applying a voltage gradient between the first and second ends of the second channel portion to electroosmotically move fluid through the second channel portion while applying substantially no voltage gradient between the first and second ends of the first channel portion, wherein the means for applying a voltage gradient between the first and second ends of the second channel portion comprises

a first electrode placed in electrical communication with the first channel junction;

a second electrode placed in electrical communication with the second end of the second channel portion; and

a power source electrically coupled to each of the first and second electrodes, whereby the power source is capable of delivering a different potential to each of the first and second electrodes.

4. The microfluidic device of claim 3, further comprising a first port in electrical communication with the first channel junction via a flow restrictive channel, the first electrode being placed in electrical contact with the first port.

5. The microfluidic device of claim 4, wherein the flow restrictive channel comprises a gel matrix disposed therein for substantially reducing fluid flow therethrough.

6. The microfluidic device of claim 4, wherein the flow restrictive channel comprises a channel connecting the port with the first channel junction, and having a substantially neutral surface charge.

7. A microfluidic device comprising:

a body structure comprising a substrate layer having at least a first microscale channel fabricated into a surface thereof; and

an integrated micropump in fluid communication with at least one microscale channel, the micropump comprising;

a first fluid containing microscale channel portion having first and second ends;

a second fluid containing microscale channel portion having first and second ends, the second channel portion having a first effective surface charge associated with walls of the second channel portion, the first end of the second channel portion in fluid communication with the first end of the first channel portion at a first channel junction;

at least a third fluid containing microscale channel portion having a first end and a second end, the first end of the third channel portion in fluid communication with the first channel junction;

a means for applying a voltage gradient between the first and second ends of the third fluid containing channel portion to electroosmotically move fluid through the third channel portion; and

a means for applying a voltage gradient between the first and second ends of the second channel portion to electroosmotically move fluid through the second channel portion, while applying substantially no voltage gradient between the first and second ends of the first channel portion.

8. The microfluidic device of claim 7, wherein the means for applying a voltage gradient between the first and second ends of the third channel comprises:

a first electrode placed in electrical communication with the first channel junction;

a second electrode placed in electrical communication with the second end of the third channel; and

a power source electrically coupled to each of the first and second electrodes, whereby the power source is capable of delivering a different potential to each of the first and second electrodes.

9. The microfluidic device of claim 7, wherein the third channel portion has a second surface charge associated with walls of the third channel portion, the second surface charge being substantially opposite to the first surface charge, and wherein the means for applying a voltage gradient between the first and second ends of the third channel portion comprises:

a first electrode in electrical communication with the second end of the second channel portion;

a second electrode in electrical communication with the second end of the third channel portion; and

a power source electrically coupled to each of the first and second electrodes, whereby the power source is capable of delivering a different voltage to each of the first and second electrodes.

10. The microfluidic device of claim 7, wherein the third channel portion comprises a smaller cross sectional area than the first channel portion.

11. A micropump, comprising:

a body comprising a substrate layer having a plurality of channel portions fabricated into a surface thereof, the channel portions including;

a first fluid containing microscale channel portion having first and second ends;

a second microscale channel portion having first and second ends, the second channel portion having a first effective surface charge associated with walls of the second channel portion, the first end of the second channel portion in fluid communication with the first end of the first channel portion at a first channel junction;

a third microscale channel portion having first and second ends, the third channel portion having a second effective surface charge associated with the walls of the third channel portion, the second effective surface charge being substantially opposite to the first effective surface charge, the first end of the third channel portion in fluid communication with the first channel junction; and

a means for applying a voltage gradient between the second end of the second channel portion and the second end of the third channel portion to electroosmotically move fluid through the second and third channel portions, while applying substantially no voltage gradient between the first and second ends of the first channel portion.

12. The micropump of claim 11, wherein the means for applying a voltage gradient comprises:

a first electrode in electrical communication with the second end of the second channel portion;

a second electrode in electrical communication with the second end of the third channel portion; and

a power source electrically coupled to each of the first and second electrodes, whereby the power source is capable of delivering a different voltage to each of the first and second electrodes.

13. The micropump of claim 11, wherein the third channel portion comprises a smaller cross sectional area than the first channel portion.

14. A micropump comprising:

a substrate comprising;

a first microscale channel portion fabricated into a surface of the substrate, the first channel portion having first and second ends, walls of the first channel portion having substantially neutral surface charge associated therewith;

a second fluid containing microscale channel portion fabricated into a surface of the substrate, the second channel portion having first and second ends, the second channel portion having a first surface charge associated with walls of the second channel portion, the first end of the second channel portion in fluid communication with the first end of the first channel portion at a first channel junction; and

a means for applying a voltage gradient between the first and second ends of the second channel portion to electroosmotically move fluid through the second channel portion.

15. A micropump comprising:

a substrate comprising;

a first microscale channel portion fabricated into a surface of the substrate, the first channel portion having first and second ends, walls of the first channel portion having substantially neutral surface charge associated therewith;

a second microscale channel portion fabricated into a surface of the substrate, the second channel portion having first and second ends, the second channel portion having a first surface charge associated with walls of the second channel portion, the first end of the second channel portion in fluid communication with the first end of the first channel portion at a first channel junction; and

a means for applying a voltage gradient between the first and second ends of the second channel portion wherein the means for applying a voltage gradient between the first and second ends of the second channel portion comprises

a first electrode placed in electrical communication with the first channel junction;

a second electrode placed in electrical communication with the second end of the second channel portion; and

a power source electrically coupled to each of the first and second electrodes, whereby the power source is capable of delivering a different potential to each of the first and second electrodes.

16. A micropump comprising:

a substrate comprising;

a first microscale portion fabricated into a surface of the substrate, the first channel portion having first and second ends, walls of the first channel portion having substantially neutral surface charge associated therewith;

a second microscale channel portion fabricated into a surface of the substrate, the second channel portion having first and second ends, the second channel portion having a first surface charge associated with walls of the second channel portion, the first end of the second channel portion in fluid communication with the first end of the first channel portion at a first channel junction; and

a means for applying a voltage gradient between the first and second ends of the second channel portion wherein the means for applying a voltage gradient between the first and second ends of the second channel portion comprises;

a first electrode placed in electrical communication with the second end of the first channel portion;

a second electrode placed in electrical communication with the second end of the second channel portion; and

a power source electrically coupled to each of the first and second electrodes, whereby the power source is capable of delivering a different potential to each of the first and second electrodes.

17. A micropump comprising:

a substrate comprising;

a first microscale portion fabricated into a surface of the substrate, the first channel portion having first and second ends, walls of the first channel portion having substantially neutral surface charge associated therewith;

a second microscale channel portion fabricated into a surface of the substrate, the second channel portion having first and second ends, the second channel portion having a first surface charge associated with walls of the second channel portion, the first end of the second channel portion in fluid communication with the first end of the first channel portion at a first channel junction;

at least a third microscale channel portion having a first end and a second end, the first end of the third channel portion in fluid communication with the first channel junction; and

a means for applying a voltage gradient between the first and second ends of the second channel portion and between the first and second ends of the third channel portion.

18. The micropump of claim 17, wherein the means for applying a voltage gradient between the first and second ends of the third channel comprises:

a first electrode placed in electrical communication with the second end of the first channel;

a second electrode placed in electrical communication with the second end of the third channel; and

a power source electrically coupled to each of the first and second electrodes, whereby the power source is capable of delivering a different potential to each of the first and second electrodes.

19. The micropump of claim 18, wherein the second electrode is placed in electrical contact with a first channel header, and wherein each of the second end of the second channel portion and the second end of the third channel portion are in fluid communication with the first channel header.

20. A microfluidic device including an integrated micropump, the device comprising:

a solid substrate;

first, second and third channel portions fabricated into a surface of the substrate, each of the first, second and third channel portions having first and second ends, respectively, the second ends of the first, second and third channel portions being in fluid communication at a first channel junction, and wherein the second and third channel portions have surface charges associated with walls of the second and third channel portions, respectively; and

a means for applying a voltage gradient between the first end of the second channel portion and the first end of the third channel portion.

21. The microfluidic device of claim 20, wherein the third channel portion has a surface charge density that is substantially less than a surface charge density of the second channel portion.

22. The microfluidic device of claim 21, wherein the surface charge density of the third channel portion is at least 20% less than the surface charge density of the second channel portion.

23. The microfluidic device of claim 21, wherein the surface charge density of the third channel portion is at least 50% less than the surface charge density of the second channel portion.

24. The microfluidic device of claim 20, wherein the third channel portion has substantially no surface charge associated with the walls of the third channel portion.

25. The microfluidic device of claim 20, wherein the second and third channel portions have substantially opposite surface charges.

26. The microfluidic device of claim 20, wherein the means for applying a voltage gradient between the first end of the second channel portion and the first end of the third channel portion comprises a first electrode disposed in electrical contact with the first end of the second channel portion and a second electrode disposed in electrical contact with the third channel portion, each of the first and second electrodes being connected to an electrical controller for delivering a different voltage to each of the first and second electrodes.

27. A method of pumping fluid in a microscale channel structure, comprising:

providing a first channel portion and a second channel portion of the channel structure, the second channel portion being in fluid communication with the first channel portion, at least the second channel portion having a surface charge associated with its walls;

applying a voltage gradient along a length of the second channel portion to produce an electroosmotically induced pressure within the second channel portion;

communicating the electroosmotically induced pressure from the second channel portion to an end of the first channel portion.

28. A method of pumping a fluid in a first microscale channel, comprising:

providing a second microscale channel portion in fluid communication with the first channel, the second channel portion having a surface charge associated with its walls;

applying a voltage gradient along a length of the second channel but not along the length of the first channel portion, whereby a fluid in the second channel portion is electroosmotically pumped into the first channel portion, thereby pumping a fluid in the first channel portion.