Microchip electrophoresis apparatus

• US 6,013,168 A
• Filed: 02/24/1998
• Issued: 01/11/2000
• Est. Priority Date: 03/03/1997
• Status: Expired due to Fees

• Current Assignee: Shimadzu Corporation
• Original Assignee: Shimadzu Corporation
• Inventors: Arai, Akihiro
• Primary Examiner(s): Warden, Sr., Robert J.
• Assistant Examiner(s): ALDAG, ANDREW J
• Application Number:
  US09/028,466
• Time in Patent Office: 686 Days
• Field of Search: 204/601, 204/602, 204/603, 204/604, 204/606, 204/612, 204/616, 422/50, 422/61, 422/67
• US Class Current: 204/601
• CPC Class Codes: G01N 27/44721 by optical means
• CPC Class Codes: G01N 27/44791 Microapparatus sample containers with integrated microfluidic structures B01L3/5027

Abstract

When a microchip is set on a tray and operation of an apparatus is begun, the microchip moves to feed position in order to be filled up with a buffer solution, where upon a sample is injected into this microchip. Thereafter the tray is located on a detecting position, so that a sample introduction voltage is applied between a sample reservoir and a sample waste reservoir for introducing the sample into a separation passage. Subsequently, the operation is switched to application of a separation voltage between a buffer reservoir and a drain reservoir, for beginning analysis. When the analysis has begun, a detector detects a migration pattern in the separation passage, so that a signal processing board data-processes the detected pattern.

Claims

1. A microchip electrophoresis apparatus employing a microchip comprising a pair of transparent plate members, a groove formed on a surface of at least one said plate member for passing a liquid therethrough and through holes provided on any one said plate member in positions corresponding to both ends of said groove, said plate members being stuck to each other while inwardly directing said groove for defining a separation passage and a sample introduction passage intersecting with each other by said groove, for filling up said separation passage and said sample introduction passage with a buffer solution, applying a sample introduction voltage across said sample introduction passage and introducing a sample from said sample introduction passage into said separation passage, and subsequently applying a separation voltage across said separation passage for electrophoretically separating said sample in said separation passage, said microchip electrophoresis apparatus comprising:

a detector for optically detecting electrophoretically separated said sample;

a moving mechanism for moving said microchip, which is horizontally positioned on a tray;

a liquid injection mechanism for injecting said buffer solution from said through holes;

a sample injection mechanism for injecting said sample from a sample injection hole of said through hole positioned on an end of said sample introduction passage;

a power source for switching and applying a sample introduction voltage for introducing said sample from said sample introduction passage into said separation passage and a separation voltage for electrophoretically separating said sample;

a control part for automatically controlling the movement of said microchip by means of said moving mechanism for locating said microchip on a series of positions which include;

a buffer solution injecting position with said liquid injection mechanism;

a sample injecting position with said sample injection mechanism and a detecting position with said detector respectively;

said control part further controlling operations of said liquid injection mechanism and said sample injection mechanism and control of voltage application with said power source;

wherein said control part comprises a program for applying a prescribed voltage across said passages of said microchip after said passages are filled up with said buffer solution and which then determines whether or not said passages are properly filled up with said buffer solution upon determining the presence of a current value; and

a case which integrally stores all these parts.

2. The microchip electrophoresis apparatus in accordance with claim 1, wherein

said transparent plate members of said microchip are made of glass, quartz or resin, electrode patterns connecting to said separation passage and said sample introduction passage through which said through holes are formed on one of said transparent plate members, and said electrode patterns connect to a voltage terminal, and

said voltage terminal is connected to a connector which is connected to said power source, thereby applying said sample introduction voltage and said separation voltage to said passages through said electrode patterns.

3. The microchip electrophoresis apparatus in accordance with claim 1, wherein

said detector is a spectrophotometric detector, comprising a spectroscope for separating light from a light source into its spectral components, an optical system for linearly condensing separated said light on said separation passage of said microchip which is located on said detecting position and irradiating said separation passage with said light, and arrayed photodetectors for simultaneously receiving light transmitted through a prescribed range of said separation passage and detecting its migration pattern.

4. The microchip electrophoresis apparatus in accordance with claim 1, wherein

said case is provided with a window on its front surface which is capable of partially exposing said tray for enabling the setting or removal of said microchip in respect to said tray.

5. The microchip electrophoresis apparatus in accordance with claim 1, wherein

said control part also comprises a program for re-locating said microchip on said buffer solution injecting position for re-filling said passages with said buffer solution upon determining that said passages have not been properly filled up with said buffer solution.

6. The microchip electrophoresis apparatus in accordance with claim 1, wherein

said microchip is so arranged that said separation passage is perpendicular to the directional movement of said microchip when moved to said detecting position with said detector, and

said control part feeds back a signal outputted from said detector when an optical axis of said detector crosses said separation passage of said microchip to said moving mechanism which moves said microchip thereby locating said microchip on said detecting position.