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Massively parallel signature sequencing by ligation of encoded adaptors

  • US 6,013,445 A
  • Filed: 10/07/1997
  • Issued: 01/11/2000
  • Est. Priority Date: 06/06/1996
  • Status: Expired
First Claim
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1. A method of determining a nucleotide sequence at an end of a polynucleotide, the method comprising the steps of:

  • ligating one or more encoded adaptors to the ends of identical copies of the polynucleotide, each encoded adaptor being a double stranded deoxyribonucleic acid comprising an oligonucleotide tag selected from a minimally cross-hybridizing set of oligonucleotides between 8 and 20 nucleotides or basepairs in length and a protruding strand complementary to a portion of a strand of the polynucleotide, wherein each oligonucleotide of the minimally cross-hybridizing set differs from every other oligonucleotide of the set by at least two nucleotides; and

    identifying one or more nucleotides in each of said portions of the strand of the polynucleotides by specifically hybridizing a tag complement to each oligonucleotide tag of the one or more encoded adaptors ligated thereto.

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