Binding proteins for recognition of DNA
First Claim
1. A library of DNA sequences, each sequence encoding a zinc finger polypeptide for display on a viral particle, the zinc finger polypeptide comprising at least three zinc fingers, with one zinc finger having a partially randomized allocation of amino acids being positioned between two or more zinc fingers having a defined amino acid sequence, the partially randomized zinc finger having random allocation of amino acids at positions -1, +2, +3 and +6, position +1 being the first amino acid in the α
- -helix of the zing finger.
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Accused Products
Abstract
Disclosed are libraries of DNA sequences encoding zinc finger binding motifs for display on a particle, together with methods of designing zinc finger binding polypeptides for binding to a particular target sequence and, inter alia, use of designed zinc finger polypeptides for various in vitro or in vivo applications.
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Citations
26 Claims
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1. A library of DNA sequences, each sequence encoding a zinc finger polypeptide for display on a viral particle, the zinc finger polypeptide comprising at least three zinc fingers, with one zinc finger having a partially randomized allocation of amino acids being positioned between two or more zinc fingers having a defined amino acid sequence, the partially randomized zinc finger having random allocation of amino acids at positions -1, +2, +3 and +6, position +1 being the first amino acid in the α
- -helix of the zing finger.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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21. A kit for making a zinc finger polypeptide for binding to a DNA of interest, comprising:
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a library of DNA sequences, each sequence encoding a zinc finger polypedtide for display on a viral particle, the zinc finger polypeptide comprising at least three zinc fingers, with one zinc finger having a partially randomized allocation of amino acids being positioned between two or more zinc fingers having a defined amino acid sequence, the partially randomized zinc finger having random allocation of amino acids at positions -1, +2, +3 and +6. position +1 being the first amino acid in the α
-helix of the zing finger;a vector molecule suitable for accepting one or more sequences from the library; and
instructions for use. - View Dependent Claims (22, 23, 24, 25, 26)
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Specification