Method for carrying out an immunoassay in a multiphase system
First Claim
1. A method for immunometrically detecting and/or determining the concentration of an analyte, comprising the steps of:
- 1) contacting a sample containing the analyte with;
a) a labeled tracer, which binds to the analyte to form a labeled complex;
b) a receptor A, which binds to the labeled complex;
c) a receptor B, which binds to the labeled tracer and generates or alters, a signal by interacting with the labeled tracer,wherein receptors A and B are immobilized at a distance sufficient to prevent all or most of the labeled complexes from simultaneously binding to both an A and a B receptor;
2) determining a signal generated by the labeled tracer;
3) analyzing the signal to detect and/or determine the concentration of analyte in the sample.
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Accused Products
Abstract
The present invention relates to a method of carrying out an immunoassay in a multiphase system. Particularly, a sample containing an analyte is brought into contact with a receptor A and a tracer. The analyte can either from a complex with the tracer, or counteract the formation of a complex of receptor A and tracer by competing with the tracer for binding to receptor A, or counteract the formation of a complex of receptor A and tracer by competing with receptor A. Receptor B is added and the signal is determined. In this method, receptor A and receptor B are suitably immobilized, ensuring that the tracer either cannot enter into any binding involving the simultaneous participation of receptors A and B or can enter into such a binding to only such a slight extent that it is nevertheless possible to detect and differentiate differing analyte concentrations.
14 Citations
28 Claims
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1. A method for immunometrically detecting and/or determining the concentration of an analyte, comprising the steps of:
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1) contacting a sample containing the analyte with; a) a labeled tracer, which binds to the analyte to form a labeled complex; b) a receptor A, which binds to the labeled complex; c) a receptor B, which binds to the labeled tracer and generates or alters, a signal by interacting with the labeled tracer, wherein receptors A and B are immobilized at a distance sufficient to prevent all or most of the labeled complexes from simultaneously binding to both an A and a B receptor; 2) determining a signal generated by the labeled tracer; 3) analyzing the signal to detect and/or determine the concentration of analyte in the sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
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28. A method for immunometrically detecting and/or determining the concentration of an analyte, comprising the steps of:
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1) contacting a sample containing the analyte with; a) a labeled tracer, which binds to the analyte to form a labeled complex; b) a receptor A, which binds to the labeled complex; c) a receptor B, which binds to the labeled tracer and generates or alters a signal by interacting with the labeled tracer, wherein receptor A and the labeled tracer are immobilized at a distance sufficient to prevent all or most of the labeled complexes from simultaneously binding to both a receptor A and to a labeled tracer; 2) determining a signal generated by the labeled tracer; and 3) analyzing the signal to detect and/or determine the concentration of analyte in the sample.
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Specification