Method for inducing a systemic immune response to an antigen
First Claim
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1. A method for stimulating a systemic immune response to an antigen in a mammal comprising:
- providing a liposomal preparation comprising lyophilized liposomes containing at least one antigen, wherein the liposomes have at least three different sizes, before lyophilization and consisting essentially of;
at least 5% by volume, based on the total value of liposomes in the preparation, small liposomes having a size, before lyophilization, of from about 20 nm to about 1 micron,at least 5% by volume, based on the total volume of liposomes in the preparation, medium liposomes having a size, before lyophilization, of from about 1 micron to about 3 microns, andat least 5% by volume, based on the total volume of liposomes in the preparation, large liposomes having a size, before lyophilization, of from about 3 microns to about 20 microns; and
orally administering an effective amount of the liposomal preparation to a mammal, whereby sufficient antigen containing liposomes are absorbed in the Peyer'"'"'s patches of the gut of the mammal and are taken up by macrophages in the Peyer'"'"'s patches to stimulate a systemic immune response.
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Abstract
A method is provided for inducing a systemic immune response to an antigen in a mammal. The method comprises orally administering lyophilized multilamellar liposomes containing the antigen. The liposomes have a size of from 20 nm to 20 microns. The antigen-containing liposomes are absorbed in the Peyer'"'"'s patches of the gut. Sufficient antigen-containing liposomes are taken up by macrophages in the Peyer'"'"'s patches to induce a systemic immune response to the antigen.
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Citations
52 Claims
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1. A method for stimulating a systemic immune response to an antigen in a mammal comprising:
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providing a liposomal preparation comprising lyophilized liposomes containing at least one antigen, wherein the liposomes have at least three different sizes, before lyophilization and consisting essentially of; at least 5% by volume, based on the total value of liposomes in the preparation, small liposomes having a size, before lyophilization, of from about 20 nm to about 1 micron, at least 5% by volume, based on the total volume of liposomes in the preparation, medium liposomes having a size, before lyophilization, of from about 1 micron to about 3 microns, and at least 5% by volume, based on the total volume of liposomes in the preparation, large liposomes having a size, before lyophilization, of from about 3 microns to about 20 microns; and orally administering an effective amount of the liposomal preparation to a mammal, whereby sufficient antigen containing liposomes are absorbed in the Peyer'"'"'s patches of the gut of the mammal and are taken up by macrophages in the Peyer'"'"'s patches to stimulate a systemic immune response. - View Dependent Claims (2, 3, 4, 5, 6, 7, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
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8. A preparation for oral administration to a mammal capable of stimulating a systemic immune response to at least one antigen, said preparation comprising an effective amount of lyophilized antigen-containing liposomes, wherein the liposomes have at least three different sizes, before lyophilization, and consist essentially of:
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at least 5% by volume, based on the total volume of liposomes in the preparation, small liposomes having a size, before lyophilization, of from about 20 nm to about 1 micron, at least 5% by volume, based on the total volume of liposomes in the preparation, medium liposomes having a sizes before lyophilization, of from about 1 micron to about 3 microns, and at least 5% by volume, based on the total volume of liposomes in the preparation, large liposomes having a size, before lyophilization, of from about 3 microns to about 20 microns. - View Dependent Claims (9, 10, 11, 12, 13, 14, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52)
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Specification